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• W3209695265 startingPage "130985" @default.
• W3209695265 abstract "Histamine (HIS) and tryptamine (TRY) are heterocyclic biogenic amines (BAs) often found in food products. The consumption of products containing high levels of BAs results in a wide range of life-threatening physiological reactions. The work described a Simultaneous coupled with Separate selection strategy for the screening of different aptamers against histamine and tryptamine. Aptamer HIS-3 and TRY-2 exhibited a good binding affinity; specificity and pH stability to histamine and tryptamine were obtained with a dissociation constant of 11.62 ± 3.241 nM and 6.303 ± 1.758 nM, respectively. The preliminary binding mechanism was analyzed using a circular dichromatic (CD) and molecular docking. Finally, the identified aptamers were used for the development of a colorimetric method for the detection of histamine and tryptamine, achieving limits of detection of 1 ng/mL and 10 ng/mL, respectively. This aptamer-based method was further successfully employed to detect the BAs in fish and pork, providing a potential application for sensitive and efficient detecting multiple food risk factors. • Simultaneous coupled with Separate SELEX was developed for aptamer screening. • Histamine and tryptamine possess similar core structure were employed as targets. • The affinity, specificity and pH stability of aptamer candidates were analyzed. • Colorimetric analysis for biogenic amine was established based on selected aptamer." @default.
• W3209695265 created "2021-11-08" @default.
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• W3209695265 date "2022-02-01" @default.
• W3209695265 modified "2023-10-16" @default.
• W3209695265 title "Simultaneous coupled with Separate SELEX for heterocyclic biogenic amine-specific aptamers screening and their application in establishment of an effective aptasensor" @default.
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• W3209695265 doi "https://doi.org/10.1016/j.snb.2021.130985" @default.
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