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• W3209776652 abstract "Lysosomal acidification is essential for its degradative function, and the flux of H+ correlated with that of K+ in lysosomes. However, there is little research on their correlation due to the lack of probes that can simultaneously image these two ions. To deeply understand the role of K+ in lysosomal acidification, here, we designed and fabricated a nanodevice using a K+-aptamer and two pH-triggered nanoswitches incorporated into a DNA triangular prism (DTP) as a dual signal response platform to simultaneously visualize K+ and pH in lysosomes by a fluorescence method. This strategy could conveniently integrate two signal recognition modules into one probe, so as to achieve the goal of sensitive detection of two kinds of signals in the same time and space, which is suitable for the detection of various signals with the correlation of concentration. By co-imaging both K+ and H+ in lysosomes, we found that the efflux of K+ was accompanied by a decrease of pH, which is of great value in understanding lysosomal acidification. Moreover, this strategy also has broad prospects as a versatile optical sensing platform for multiplexed analysis of other biomolecules in living cells." @default.
• W3209776652 created "2021-11-08" @default.
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• W3209776652 date "2021-10-28" @default.
• W3209776652 modified "2023-10-16" @default.
• W3209776652 title "Dual Recognition DNA Triangular Prism Nanoprobe: Toward the Relationship between K⁺ and pH in Lysosomes" @default.
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• W3209776652 doi "https://doi.org/10.1021/acs.analchem.1c04056" @default.
• W3209776652 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34709789" @default.
• W3209776652 hasPublicationYear "2021" @default.
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