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- W3209953001 abstract "Kapur (Dryobalanops aromatica) is an important dipterocarp species currently classified as vulnerable by the IUCN Red List Threatened Species. Science-based conservation and restoration efforts are needed, which can be supported by new genomic data generated from new technologies, including MinION Oxford Nanopore Technology (ONT). ONT allows affordable long-read DNA sequencing, but this technology is still rarely applied to native Indonesian forest trees. Therefore, this study aimed to generate whole genome datasets through ONT and use part of these data to construct the draft of the chloroplast genome and analyze the universal DNA barcode-based genetic relationships for D. aromatica. The method included DNA isolation, library preparation, sequencing, bioinformatics analysis, and phylogenetic tree construction. Results showed that the DNA sequencing of D. aromatica resulted in 1.55 Gb of long-read DNA sequences from which a partial chloroplast genome (148,856 bp) was successfully constructed. The genetic relationship was analyzed using two selected DNA barcodes (rbcL and matK), and its combination showed that species of the genus Dryobalanops had a close relationship as indicated by adjacent branches between species. The phylogenetic tree of matK and the combination of the matK and rbcL genes showed that D. aromatica was closely related to Dryobalanops rappa, whereas the rbcL gene showed group separation between D. aromatica and D. rappa. Therefore, a combination of the matK and rbcL genes is recommended for future use in the phylogenetic or phylogenomic analysis of D. aromatica." @default.
- W3209953001 created "2021-11-08" @default.
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- W3209953001 date "2021-11-03" @default.
- W3209953001 modified "2023-10-04" @default.
- W3209953001 title "Chloroplast Genome Draft of Dryobalanops aromatica Generated Using Oxford Nanopore Technology and Its Potential Application for Phylogenetic Study" @default.
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- W3209953001 cites W1939917798 @default.
- W3209953001 cites W1966029203 @default.
- W3209953001 cites W1982005102 @default.
- W3209953001 cites W2002420088 @default.
- W3209953001 cites W2023028439 @default.
- W3209953001 cites W2027594296 @default.
- W3209953001 cites W2030467655 @default.
- W3209953001 cites W2043339834 @default.
- W3209953001 cites W2050474297 @default.
- W3209953001 cites W2057719575 @default.
- W3209953001 cites W2071510336 @default.
- W3209953001 cites W2078044071 @default.
- W3209953001 cites W2106151976 @default.
- W3209953001 cites W2110883875 @default.
- W3209953001 cites W2132081049 @default.
- W3209953001 cites W2169557206 @default.
- W3209953001 cites W2189047836 @default.
- W3209953001 cites W2252349061 @default.
- W3209953001 cites W2314991621 @default.
- W3209953001 cites W2324346851 @default.
- W3209953001 cites W2328076556 @default.
- W3209953001 cites W2345802980 @default.
- W3209953001 cites W2480071061 @default.
- W3209953001 cites W2482723931 @default.
- W3209953001 cites W2521004200 @default.
- W3209953001 cites W2561122916 @default.
- W3209953001 cites W2605414348 @default.
- W3209953001 cites W2612261225 @default.
- W3209953001 cites W2744971417 @default.
- W3209953001 cites W2759402232 @default.
- W3209953001 cites W2767716491 @default.
- W3209953001 cites W2772019197 @default.
- W3209953001 cites W2772689352 @default.
- W3209953001 cites W2790836809 @default.
- W3209953001 cites W2795630369 @default.
- W3209953001 cites W2799524357 @default.
- W3209953001 cites W2803760314 @default.
- W3209953001 cites W2803900966 @default.
- W3209953001 cites W2808741946 @default.
- W3209953001 cites W2884116482 @default.
- W3209953001 cites W2884978946 @default.
- W3209953001 cites W2886835318 @default.
- W3209953001 cites W2906417798 @default.
- W3209953001 cites W2917687614 @default.
- W3209953001 cites W2923551970 @default.
- W3209953001 cites W2948183769 @default.
- W3209953001 cites W2952091873 @default.
- W3209953001 cites W2956730582 @default.
- W3209953001 cites W2972944557 @default.
- W3209953001 cites W2981091732 @default.
- W3209953001 cites W2982391841 @default.
- W3209953001 cites W3007172120 @default.
- W3209953001 cites W3016835565 @default.
- W3209953001 cites W3025532513 @default.
- W3209953001 cites W3027327745 @default.
- W3209953001 cites W3048090444 @default.
- W3209953001 cites W3053873271 @default.
- W3209953001 cites W3093660670 @default.
- W3209953001 cites W3094931743 @default.
- W3209953001 cites W3122152129 @default.
- W3209953001 cites W3123306417 @default.
- W3209953001 cites W3129099547 @default.
- W3209953001 cites W3133913176 @default.
- W3209953001 cites W3133990339 @default.
- W3209953001 cites W3136638484 @default.
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- W3209953001 cites W3156458251 @default.
- W3209953001 cites W3157515622 @default.
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- W3209953001 cites W3169393977 @default.
- W3209953001 cites W3177929462 @default.
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- W3209953001 doi "https://doi.org/10.3390/f12111515" @default.
- W3209953001 hasPublicationYear "2021" @default.
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