Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210029215> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W3210029215 endingPage "109938" @default.
- W3210029215 startingPage "109938" @default.
- W3210029215 abstract "Hydrolytic enzymes are highly demanded in the industry. Thermostability is an important property of enzymes that affects the economic costs of the industrial processes. The rational design of GH10 xylanase E (XylE) Penicillium canescens for the thermostability improvement was directed by ΔΔG calculations and structure analysis. Amino acid substitutions with stabilizing values of ΔΔG and providing an increase in side-chain volume of buried residues were performed experimentally. From the six designed substitutions, four substitutions appeared to be stabilizing, one - destabilizing, and one - neutral. For the improved XylE variants, values of Tm were increased by 1.1-3.1 °C, and times of half-life at 70 °C were increased in 1.3-1.7-times. Three of the four stabilizing substitutions were located in the N- or the C-terminus region. This highlights the importance of N- and C-terminus for the thermostability of GH10 xylanases and also enzymes with (β/α)8 TIM barrel type of structure. The criteria of stabilizing values of ΔΔG and increased side-chain volume of buried residues for selection of substitutions may be applied in the rational design for thermostability improvement." @default.
- W3210029215 created "2021-11-08" @default.
- W3210029215 creator A5001797092 @default.
- W3210029215 creator A5018714983 @default.
- W3210029215 creator A5021992596 @default.
- W3210029215 creator A5040978146 @default.
- W3210029215 creator A5062641864 @default.
- W3210029215 creator A5067728398 @default.
- W3210029215 date "2021-12-01" @default.
- W3210029215 modified "2023-10-16" @default.
- W3210029215 title "Enhancement of thermostability of GH10 xylanase E Penicillium canescens directed by ΔΔG calculations and structure analysis" @default.
- W3210029215 cites W1548662682 @default.
- W3210029215 cites W2010670537 @default.
- W3210029215 cites W2026877256 @default.
- W3210029215 cites W2029546435 @default.
- W3210029215 cites W2029667189 @default.
- W3210029215 cites W2056964739 @default.
- W3210029215 cites W2057945340 @default.
- W3210029215 cites W2088534336 @default.
- W3210029215 cites W2099187197 @default.
- W3210029215 cites W2101904220 @default.
- W3210029215 cites W2103459989 @default.
- W3210029215 cites W2111004301 @default.
- W3210029215 cites W2149982635 @default.
- W3210029215 cites W2152756549 @default.
- W3210029215 cites W2286311455 @default.
- W3210029215 cites W2295369589 @default.
- W3210029215 cites W2376573086 @default.
- W3210029215 cites W2409584551 @default.
- W3210029215 cites W2460469344 @default.
- W3210029215 cites W2500213943 @default.
- W3210029215 cites W2508856013 @default.
- W3210029215 cites W2588135422 @default.
- W3210029215 cites W2605045174 @default.
- W3210029215 cites W2715003498 @default.
- W3210029215 cites W2776963100 @default.
- W3210029215 cites W2782149754 @default.
- W3210029215 cites W2789564237 @default.
- W3210029215 cites W2977696832 @default.
- W3210029215 cites W2985105787 @default.
- W3210029215 cites W2987691864 @default.
- W3210029215 cites W3047967535 @default.
- W3210029215 cites W3048495640 @default.
- W3210029215 cites W3117622225 @default.
- W3210029215 cites W3191919773 @default.
- W3210029215 cites W778581746 @default.
- W3210029215 doi "https://doi.org/10.1016/j.enzmictec.2021.109938" @default.
- W3210029215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34753033" @default.
- W3210029215 hasPublicationYear "2021" @default.
- W3210029215 type Work @default.
- W3210029215 sameAs 3210029215 @default.
- W3210029215 citedByCount "2" @default.
- W3210029215 countsByYear W32100292152022 @default.
- W3210029215 crossrefType "journal-article" @default.
- W3210029215 hasAuthorship W3210029215A5001797092 @default.
- W3210029215 hasAuthorship W3210029215A5018714983 @default.
- W3210029215 hasAuthorship W3210029215A5021992596 @default.
- W3210029215 hasAuthorship W3210029215A5040978146 @default.
- W3210029215 hasAuthorship W3210029215A5062641864 @default.
- W3210029215 hasAuthorship W3210029215A5067728398 @default.
- W3210029215 hasConcept C181199279 @default.
- W3210029215 hasConcept C185592680 @default.
- W3210029215 hasConcept C2775859485 @default.
- W3210029215 hasConcept C2781216036 @default.
- W3210029215 hasConcept C55493867 @default.
- W3210029215 hasConcept C71240020 @default.
- W3210029215 hasConcept C86803240 @default.
- W3210029215 hasConceptScore W3210029215C181199279 @default.
- W3210029215 hasConceptScore W3210029215C185592680 @default.
- W3210029215 hasConceptScore W3210029215C2775859485 @default.
- W3210029215 hasConceptScore W3210029215C2781216036 @default.
- W3210029215 hasConceptScore W3210029215C55493867 @default.
- W3210029215 hasConceptScore W3210029215C71240020 @default.
- W3210029215 hasConceptScore W3210029215C86803240 @default.
- W3210029215 hasLocation W32100292151 @default.
- W3210029215 hasLocation W32100292152 @default.
- W3210029215 hasOpenAccess W3210029215 @default.
- W3210029215 hasPrimaryLocation W32100292151 @default.
- W3210029215 hasRelatedWork W2074987689 @default.
- W3210029215 hasRelatedWork W2094336165 @default.
- W3210029215 hasRelatedWork W2167611972 @default.
- W3210029215 hasRelatedWork W2362777130 @default.
- W3210029215 hasRelatedWork W2373154855 @default.
- W3210029215 hasRelatedWork W2508856013 @default.
- W3210029215 hasRelatedWork W2898594562 @default.
- W3210029215 hasRelatedWork W4240696276 @default.
- W3210029215 hasRelatedWork W4295102914 @default.
- W3210029215 hasRelatedWork W2282989737 @default.
- W3210029215 hasVolume "152" @default.
- W3210029215 isParatext "false" @default.
- W3210029215 isRetracted "false" @default.
- W3210029215 magId "3210029215" @default.
- W3210029215 workType "article" @default.