Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210069894> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W3210069894 abstract "Background: Mannheimia haemolytica is the major bacterial infectious agent of bovine respiratory disease complex and causes severe morbidity and mortality during lung infections. M. haemolytica secretes a protein leukotoxin (Lkt) that binds to the CD18 receptor on leukocytes, initiates lysis, induces inflammation, and causes acute fibrinous bronchopneumonia. Lkt binds the 22-amino acid CD18 signal peptide domain, which remains uncleaved in ruminant species. Our aim was to identify missense variation in the bovine CD18 signal peptide and measure the effects on Lkt binding. Methods: Missense variants in the integrin beta 2 gene (ITGB2) encoding CD18 were identified by whole genome sequencing of 96 cattle from 19 breeds, and targeted Sanger sequencing of 1238 cattle from 46 breeds. The ability of different CD18 signal peptide variants to bind Lkt was evaluated by preincubating the toxin with synthetic peptides and applying the mixture to susceptible bovine cell cultures in cytotoxicity-blocking assays. Results: We identified 14 missense variants encoded on 15 predicted haplotypes, including a rare signal peptide variant with a cysteine at position 5 (C5) instead of arginine (R5). Preincubating Lkt with synthetic signal peptides with C5 blocked cytotoxicity significantly better than those with R5. The most potent synthetic peptide (C5 PQLLLLAGLLA) had 30-fold more binding activity compared to that with R5. Conclusions: The results suggest that missense variants in the CD18 signal peptide affect Lkt binding, and animals carrying the C5 allele may be more susceptible to the effects of Lkt. The results also identify a potent class of non-antibiotic Lkt inhibitors that could potentially protect cattle from cytotoxic effects during acute lung infections." @default.
- W3210069894 created "2021-11-08" @default.
- W3210069894 creator A5051723827 @default.
- W3210069894 creator A5051921729 @default.
- W3210069894 creator A5055193789 @default.
- W3210069894 creator A5063209634 @default.
- W3210069894 creator A5064402534 @default.
- W3210069894 creator A5066777905 @default.
- W3210069894 creator A5068336925 @default.
- W3210069894 date "2018-01-01" @default.
- W3210069894 modified "2023-09-27" @default.
- W3210069894 title "A bovine CD18 signal peptide variant with increased binding activity to Mannheimia hemolytica leukotoxin [version 1; peer review: 3 approved" @default.
- W3210069894 hasPublicationYear "2018" @default.
- W3210069894 type Work @default.
- W3210069894 sameAs 3210069894 @default.
- W3210069894 citedByCount "0" @default.
- W3210069894 crossrefType "journal-article" @default.
- W3210069894 hasAuthorship W3210069894A5051723827 @default.
- W3210069894 hasAuthorship W3210069894A5051921729 @default.
- W3210069894 hasAuthorship W3210069894A5055193789 @default.
- W3210069894 hasAuthorship W3210069894A5063209634 @default.
- W3210069894 hasAuthorship W3210069894A5064402534 @default.
- W3210069894 hasAuthorship W3210069894A5066777905 @default.
- W3210069894 hasAuthorship W3210069894A5068336925 @default.
- W3210069894 hasConcept C104317684 @default.
- W3210069894 hasConcept C10858879 @default.
- W3210069894 hasConcept C167625842 @default.
- W3210069894 hasConcept C2779281246 @default.
- W3210069894 hasConcept C501734568 @default.
- W3210069894 hasConcept C55493867 @default.
- W3210069894 hasConcept C75563809 @default.
- W3210069894 hasConcept C86803240 @default.
- W3210069894 hasConcept C89423630 @default.
- W3210069894 hasConceptScore W3210069894C104317684 @default.
- W3210069894 hasConceptScore W3210069894C10858879 @default.
- W3210069894 hasConceptScore W3210069894C167625842 @default.
- W3210069894 hasConceptScore W3210069894C2779281246 @default.
- W3210069894 hasConceptScore W3210069894C501734568 @default.
- W3210069894 hasConceptScore W3210069894C55493867 @default.
- W3210069894 hasConceptScore W3210069894C75563809 @default.
- W3210069894 hasConceptScore W3210069894C86803240 @default.
- W3210069894 hasConceptScore W3210069894C89423630 @default.
- W3210069894 hasLocation W32100698941 @default.
- W3210069894 hasOpenAccess W3210069894 @default.
- W3210069894 hasPrimaryLocation W32100698941 @default.
- W3210069894 hasRelatedWork W1486112923 @default.
- W3210069894 hasRelatedWork W1894176765 @default.
- W3210069894 hasRelatedWork W1922582068 @default.
- W3210069894 hasRelatedWork W1961219082 @default.
- W3210069894 hasRelatedWork W1983605577 @default.
- W3210069894 hasRelatedWork W1989716037 @default.
- W3210069894 hasRelatedWork W2069923622 @default.
- W3210069894 hasRelatedWork W2087688988 @default.
- W3210069894 hasRelatedWork W2105277776 @default.
- W3210069894 hasRelatedWork W2110157686 @default.
- W3210069894 hasRelatedWork W2123693893 @default.
- W3210069894 hasRelatedWork W2155894257 @default.
- W3210069894 hasRelatedWork W2165513104 @default.
- W3210069894 hasRelatedWork W2171134740 @default.
- W3210069894 hasRelatedWork W2545963238 @default.
- W3210069894 hasRelatedWork W2889151996 @default.
- W3210069894 hasRelatedWork W2907795676 @default.
- W3210069894 hasRelatedWork W3199507053 @default.
- W3210069894 hasRelatedWork W1815951117 @default.
- W3210069894 hasRelatedWork W45097455 @default.
- W3210069894 isParatext "false" @default.
- W3210069894 isRetracted "false" @default.
- W3210069894 magId "3210069894" @default.
- W3210069894 workType "article" @default.