Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210135275> ?p ?o ?g. }
- W3210135275 endingPage "2232" @default.
- W3210135275 startingPage "2232" @default.
- W3210135275 abstract "Bordetella avium (BA) is one of many pathogens that cause respiratory diseases in turkeys. However, other bacterial species can easily overgrow it during isolation attempts. This makes confirming the diagnosis of BA as the causative agent of turkey coryza more difficult. Currently, there are two PCR assays for the molecular detection of BA. One is conventional gel-based PCR and the other is TaqMan real-time PCR (qPCR) assay. However, multiple pitfalls were detected in both assays regarding their specificity, sensitivity, and efficiency, which limits their utility as diagnostic tools. In this study, we developed and validated two TaqMan qPCR assays and compared their performance to the currently available TaqMan qPCR. The two assays were able to correctly identify all BA isolates and showed negative results against a wide range of different microorganisms. The two assays were found to have high efficiency with a detection limit of approximately 1 × 103 plasmid DNA Copies/mL with high repeatability and reproducibility. In comparison to the currently available TaqMan qPCR assay, the newly developed assays showed significantly higher PCR efficiencies due to superior primers and probes design. The new assays can serve as a reliable tool for the sensitive, specific, and efficient diagnosis of BA." @default.
- W3210135275 created "2021-11-08" @default.
- W3210135275 creator A5008385579 @default.
- W3210135275 creator A5024317849 @default.
- W3210135275 creator A5045115179 @default.
- W3210135275 creator A5050151675 @default.
- W3210135275 creator A5057261637 @default.
- W3210135275 creator A5076149025 @default.
- W3210135275 date "2021-10-27" @default.
- W3210135275 modified "2023-10-18" @default.
- W3210135275 title "Development and Validation of Two Diagnostic Real-Time PCR (TaqMan) Assays for the Detection of Bordetella avium from Clinical Samples and Comparison to the Currently Available Real-Time TaqMan PCR Assay" @default.
- W3210135275 cites W1866797630 @default.
- W3210135275 cites W1973650701 @default.
- W3210135275 cites W1980741999 @default.
- W3210135275 cites W2010425810 @default.
- W3210135275 cites W2012129295 @default.
- W3210135275 cites W2018255407 @default.
- W3210135275 cites W2023670773 @default.
- W3210135275 cites W2032019813 @default.
- W3210135275 cites W2034184054 @default.
- W3210135275 cites W2045523100 @default.
- W3210135275 cites W2051575239 @default.
- W3210135275 cites W2052544905 @default.
- W3210135275 cites W2055043387 @default.
- W3210135275 cites W2058576088 @default.
- W3210135275 cites W2059862007 @default.
- W3210135275 cites W2068722873 @default.
- W3210135275 cites W2070019379 @default.
- W3210135275 cites W2080322733 @default.
- W3210135275 cites W2085128682 @default.
- W3210135275 cites W2089743828 @default.
- W3210135275 cites W2097393009 @default.
- W3210135275 cites W2100187217 @default.
- W3210135275 cites W2103740237 @default.
- W3210135275 cites W2106882534 @default.
- W3210135275 cites W2111738712 @default.
- W3210135275 cites W2119318184 @default.
- W3210135275 cites W2131986125 @default.
- W3210135275 cites W2132911898 @default.
- W3210135275 cites W2140085663 @default.
- W3210135275 cites W2144510009 @default.
- W3210135275 cites W2145526497 @default.
- W3210135275 cites W2149903385 @default.
- W3210135275 cites W2152144892 @default.
- W3210135275 cites W2163274427 @default.
- W3210135275 cites W2168420558 @default.
- W3210135275 cites W2170654719 @default.
- W3210135275 cites W2188440515 @default.
- W3210135275 cites W2251344054 @default.
- W3210135275 cites W2413198769 @default.
- W3210135275 cites W2580359148 @default.
- W3210135275 cites W2584810127 @default.
- W3210135275 cites W2601663145 @default.
- W3210135275 cites W2615126908 @default.
- W3210135275 cites W2761187146 @default.
- W3210135275 cites W2768784295 @default.
- W3210135275 cites W2799524357 @default.
- W3210135275 cites W2909571268 @default.
- W3210135275 cites W2922164532 @default.
- W3210135275 cites W2961668574 @default.
- W3210135275 cites W2968636115 @default.
- W3210135275 cites W2971161287 @default.
- W3210135275 cites W2980214501 @default.
- W3210135275 cites W3021165906 @default.
- W3210135275 cites W3033394200 @default.
- W3210135275 cites W4237984257 @default.
- W3210135275 cites W1978567559 @default.
- W3210135275 doi "https://doi.org/10.3390/microorganisms9112232" @default.
- W3210135275 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8619015" @default.
- W3210135275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34835358" @default.
- W3210135275 hasPublicationYear "2021" @default.
- W3210135275 type Work @default.
- W3210135275 sameAs 3210135275 @default.
- W3210135275 citedByCount "6" @default.
- W3210135275 countsByYear W32101352752022 @default.
- W3210135275 countsByYear W32101352752023 @default.
- W3210135275 crossrefType "journal-article" @default.
- W3210135275 hasAuthorship W3210135275A5008385579 @default.
- W3210135275 hasAuthorship W3210135275A5024317849 @default.
- W3210135275 hasAuthorship W3210135275A5045115179 @default.
- W3210135275 hasAuthorship W3210135275A5050151675 @default.
- W3210135275 hasAuthorship W3210135275A5057261637 @default.
- W3210135275 hasAuthorship W3210135275A5076149025 @default.
- W3210135275 hasBestOaLocation W32101352751 @default.
- W3210135275 hasConcept C104317684 @default.
- W3210135275 hasConcept C119128265 @default.
- W3210135275 hasConcept C142724271 @default.
- W3210135275 hasConcept C143660080 @default.
- W3210135275 hasConcept C153911025 @default.
- W3210135275 hasConcept C159047783 @default.
- W3210135275 hasConcept C185592680 @default.
- W3210135275 hasConcept C204787440 @default.
- W3210135275 hasConcept C2777107318 @default.
- W3210135275 hasConcept C2780367336 @default.
- W3210135275 hasConcept C43617362 @default.
- W3210135275 hasConcept C48023723 @default.
- W3210135275 hasConcept C49105822 @default.
- W3210135275 hasConcept C523546767 @default.