Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210341010> ?p ?o ?g. }
- W3210341010 abstract "In the last two decades, methods to incorporate non-canonical amino acids (ncAAs) into specific positions of a protein have advanced significantly; these methods have become general tools for engineering proteins. However, almost all these methods depend on the translation elongation process, and strategies leveraging the initiation process have rarely been reported. The incorporation of a ncAA specifically at the translation initiation site enables the installation of reactive groups for modification at the N-termini of proteins, which are attractive positions for introducing abiological groups with minimal structural perturbations. In this study, we attempted to engineer an orthogonal protein translation initiation system. Introduction of the identity elements of Escherichia coli initiator tRNA converted an engineered Methanococcus jannaschii tRNATyr into an initiator tRNA. The engineered tRNA enabled the site-specific incorporation of O-propargyl-l-tyrosine (OpgY) into the amber (TAG) codon at the translation initiation position but was inactive toward the elongational TAG codon. Misincorporation of Gln was detected, and the engineered system was demonstrated only with OpgY. We expect further engineering of the initiator tRNA for improved activity and specificity to generate an orthogonal translation initiation system." @default.
- W3210341010 created "2021-11-08" @default.
- W3210341010 creator A5018906344 @default.
- W3210341010 creator A5032145500 @default.
- W3210341010 creator A5034150631 @default.
- W3210341010 creator A5050946440 @default.
- W3210341010 creator A5056072660 @default.
- W3210341010 date "2021-10-26" @default.
- W3210341010 modified "2023-09-29" @default.
- W3210341010 title "Towards Engineering an Orthogonal Protein Translation Initiation System" @default.
- W3210341010 cites W1561858598 @default.
- W3210341010 cites W1566760837 @default.
- W3210341010 cites W162928644 @default.
- W3210341010 cites W1666992502 @default.
- W3210341010 cites W1970743827 @default.
- W3210341010 cites W1971628354 @default.
- W3210341010 cites W1975171282 @default.
- W3210341010 cites W1985370670 @default.
- W3210341010 cites W1986826865 @default.
- W3210341010 cites W1992481862 @default.
- W3210341010 cites W1993870692 @default.
- W3210341010 cites W2018525943 @default.
- W3210341010 cites W2024736803 @default.
- W3210341010 cites W2024790906 @default.
- W3210341010 cites W2031448311 @default.
- W3210341010 cites W2034988961 @default.
- W3210341010 cites W2036284784 @default.
- W3210341010 cites W2044805176 @default.
- W3210341010 cites W2058426472 @default.
- W3210341010 cites W2062017343 @default.
- W3210341010 cites W2070282429 @default.
- W3210341010 cites W2072675235 @default.
- W3210341010 cites W2090897179 @default.
- W3210341010 cites W2102755926 @default.
- W3210341010 cites W2103563524 @default.
- W3210341010 cites W2104722248 @default.
- W3210341010 cites W2106687445 @default.
- W3210341010 cites W2107523606 @default.
- W3210341010 cites W2109155760 @default.
- W3210341010 cites W2117508167 @default.
- W3210341010 cites W2120498503 @default.
- W3210341010 cites W2140665471 @default.
- W3210341010 cites W2146913917 @default.
- W3210341010 cites W2148313902 @default.
- W3210341010 cites W2148950337 @default.
- W3210341010 cites W2163076661 @default.
- W3210341010 cites W2165286214 @default.
- W3210341010 cites W2396732095 @default.
- W3210341010 cites W2542313139 @default.
- W3210341010 cites W2573666190 @default.
- W3210341010 cites W2588611836 @default.
- W3210341010 cites W2670390373 @default.
- W3210341010 cites W2763278454 @default.
- W3210341010 cites W2896099289 @default.
- W3210341010 cites W2953759951 @default.
- W3210341010 cites W2982700634 @default.
- W3210341010 cites W2995463657 @default.
- W3210341010 cites W3004786459 @default.
- W3210341010 cites W3153152948 @default.
- W3210341010 doi "https://doi.org/10.3389/fchem.2021.772648" @default.
- W3210341010 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8576571" @default.
- W3210341010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34765589" @default.
- W3210341010 hasPublicationYear "2021" @default.
- W3210341010 type Work @default.
- W3210341010 sameAs 3210341010 @default.
- W3210341010 citedByCount "2" @default.
- W3210341010 countsByYear W32103410102022 @default.
- W3210341010 crossrefType "journal-article" @default.
- W3210341010 hasAuthorship W3210341010A5018906344 @default.
- W3210341010 hasAuthorship W3210341010A5032145500 @default.
- W3210341010 hasAuthorship W3210341010A5034150631 @default.
- W3210341010 hasAuthorship W3210341010A5050946440 @default.
- W3210341010 hasAuthorship W3210341010A5056072660 @default.
- W3210341010 hasBestOaLocation W32103410101 @default.
- W3210341010 hasConcept C104317684 @default.
- W3210341010 hasConcept C105580179 @default.
- W3210341010 hasConcept C12554922 @default.
- W3210341010 hasConcept C147816474 @default.
- W3210341010 hasConcept C149364088 @default.
- W3210341010 hasConcept C153957851 @default.
- W3210341010 hasConcept C181199279 @default.
- W3210341010 hasConcept C185592680 @default.
- W3210341010 hasConcept C2776878370 @default.
- W3210341010 hasConcept C3675279 @default.
- W3210341010 hasConcept C4718897 @default.
- W3210341010 hasConcept C515207424 @default.
- W3210341010 hasConcept C547475151 @default.
- W3210341010 hasConcept C55493867 @default.
- W3210341010 hasConcept C67705224 @default.
- W3210341010 hasConcept C70721500 @default.
- W3210341010 hasConcept C86803240 @default.
- W3210341010 hasConceptScore W3210341010C104317684 @default.
- W3210341010 hasConceptScore W3210341010C105580179 @default.
- W3210341010 hasConceptScore W3210341010C12554922 @default.
- W3210341010 hasConceptScore W3210341010C147816474 @default.
- W3210341010 hasConceptScore W3210341010C149364088 @default.
- W3210341010 hasConceptScore W3210341010C153957851 @default.
- W3210341010 hasConceptScore W3210341010C181199279 @default.
- W3210341010 hasConceptScore W3210341010C185592680 @default.
- W3210341010 hasConceptScore W3210341010C2776878370 @default.