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- W3210448977 abstract "Invariant natural killer T (iNKT) cells develop in thymus before emigrating and settling peripheral tissues and organs. In contrast to regular naïve T cells, most iNKT cells do not continuously recirculate but are rather sessile and can adopt phenotypically as well as functionally to their tissue environment. To explore this in more detail, we focused on the most widely distributed CD4+iNKT1 cells and compared the transcriptome of cells isolated from liver and spleen. Whereas there are only very few genuine differences in the transcriptomes of CD4+iNKT1 cells of these two organs, the mode of cell isolation left clear marks in the transcriptomic signature. In contrast to liver cell isolated in the cold, cells prepared by enzymatic tissue digestion upregulated quickly a series of genes known to respond to stress. Therefore, to avoid erroneous conclusions, a comparison of expression profiles must take into consideration the history of cell preparation." @default.
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- W3210448977 date "2021-12-01" @default.
- W3210448977 modified "2023-10-18" @default.
- W3210448977 title "The impact of stress on the transcriptomic signature of iNKT1 cells" @default.
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- W3210448977 doi "https://doi.org/10.1016/j.bbrep.2021.101163" @default.
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