FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210659493> ?p ?o ?g. }

• W3210659493 endingPage "64" @default.
• W3210659493 startingPage "52" @default.
• W3210659493 abstract "Septic acute kidney injury (AKI) is a severe illness in clinics. Enriching researches investigated the regulatory network of AKI during the past decades, evidences showed that circular RNAs (circRNAs) were involved in the molecular mechanism of human AKI. However, the special responses remain largely elusive. Thus, the study aims to investigate the function of circ_0114427 in the progression of AKI.The levels of circ_0114427, miR-495-3p and Tumour Necrosis Factor Receptor-Associated Factor 6 (TRAF6) were both assessed by quantitative real-time polymerase chain reaction (qRT-PCR). In addition, lipopolysaccharide (LPS) was applied to establish AKI cell model, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was carried out to determine the viability of LPS-induced HK-2 cells. The expression of TRAF6, B-cell lymphoma-2 (Bcl-2), Bcl2-associated X (Bax), cleave-caspase 3, caspase 3, total IκBα (t-IκBα), phospho-IκBα (p-IκBα), total p65 (t-p65) and phospho-p65 (p-p65) were all detected via western blot. The levels of IL-1β and TNF-α were identified by western blot and ELISA. What's more, cell apoptosis was measured by flow cytometry. Lastly, dual-luciferase reporter, RNA Immunoprecipitation (RIP) and RNA pull-down assays were employed to verify the relationships between miR-495-3p and circ_0114427 or TRAF6 in vitro.The level of miR-495-3p was remarkably restrained while circ_0114427 and TRAF6 levels were specially reinforced in AKI patient serum samples and LPS-induced HK-2 cells. Moreover, IL-1β and TNF-α were highly expressed in LPS-induced AKI cells. Functionally, circ_0114427 was a sponge of miR-495-3p, and circ_0114427 silence-mediated effects in LPS-induced HK-2 cells were partly ameliorated by the addition of miR-495-3p inhibitor. Moreover, TRAF6 was a target gene of miR-495-3p, and the inhibiting effect of miR-495-3p on cell apoptosis and inflammatory response was mitigated by TRAF6 overexpression. Mechanistically, the circ_0114427/miR-495-3p/TRAF6 axis modulated cell apoptosis and inflammatory response via NF-κB/p65 signalling pathway in AKI.Circ_0114427 regulated cell apoptosis and inflammatory response through miR-495-3p/TRAF6 axis via NF-κB/p65 signalling pathway, providing a novel mechanism in clinical treatment of AKI patients.HighlightsCirc_0114427 is upregulated in serum specimens from septic AKI patients and LPS-induced HK-2 cells.LPS treatment suppresses cell viability and promotes apoptosis and inflammation in HK-2 cells.Circ_0114427 knockdown ameliorates the effects of LPS on cell viability, apoptosis and inflammation in HK-2 cells.Circ_0114427 regulates LPS-induced HK-2 cell injury by regulating miR-495-3p/TRAF6/NF-κB/p65 axis." @default.
• W3210659493 created "2021-11-08" @default.
• W3210659493 creator A5030309010 @default.
• W3210659493 creator A5068864315 @default.
• W3210659493 creator A5077234337 @default.
• W3210659493 creator A5080582773 @default.
• W3210659493 creator A5086038672 @default.
• W3210659493 date "2021-11-03" @default.
• W3210659493 modified "2023-10-16" @default.
• W3210659493 title "Circ_0114427 promotes LPS-induced septic acute kidney injury by modulating miR-495-3p/TRAF6 through the NF-κB pathway" @default.
• W3210659493 cites W1518879205 @default.
• W3210659493 cites W1601356457 @default.
• W3210659493 cites W1866220599 @default.
• W3210659493 cites W1963906441 @default.
• W3210659493 cites W1982062130 @default.
• W3210659493 cites W1992157860 @default.
• W3210659493 cites W1997685431 @default.
• W3210659493 cites W2000356500 @default.
• W3210659493 cites W2003982318 @default.
• W3210659493 cites W2007251599 @default.
• W3210659493 cites W2007810526 @default.
• W3210659493 cites W2009968017 @default.
• W3210659493 cites W2032368204 @default.
• W3210659493 cites W2039676823 @default.
• W3210659493 cites W2047102263 @default.
• W3210659493 cites W2047967134 @default.
• W3210659493 cites W2064711537 @default.
• W3210659493 cites W2069136947 @default.
• W3210659493 cites W2075863664 @default.
• W3210659493 cites W2100292802 @default.
• W3210659493 cites W2100334598 @default.
• W3210659493 cites W2103490409 @default.
• W3210659493 cites W2104517740 @default.
• W3210659493 cites W2109435542 @default.
• W3210659493 cites W2132892038 @default.
• W3210659493 cites W2149385948 @default.
• W3210659493 cites W2149687213 @default.
• W3210659493 cites W2155763709 @default.
• W3210659493 cites W2155886511 @default.
• W3210659493 cites W2158686528 @default.
• W3210659493 cites W2163900716 @default.
• W3210659493 cites W2164990391 @default.
• W3210659493 cites W2165158988 @default.
• W3210659493 cites W2165966376 @default.
• W3210659493 cites W2470277042 @default.
• W3210659493 cites W2749922856 @default.
• W3210659493 cites W2885229294 @default.
• W3210659493 cites W2896724865 @default.
• W3210659493 cites W2967043226 @default.
• W3210659493 cites W2969913778 @default.
• W3210659493 cites W3000554746 @default.
• W3210659493 cites W3081160259 @default.
• W3210659493 cites W3123419559 @default.
• W3210659493 cites W3147406464 @default.
• W3210659493 doi "https://doi.org/10.1080/08916934.2021.1995861" @default.
• W3210659493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34730059" @default.
• W3210659493 hasPublicationYear "2021" @default.
• W3210659493 type Work @default.
• W3210659493 sameAs 3210659493 @default.
• W3210659493 citedByCount "8" @default.
• W3210659493 countsByYear W32106594932022 @default.
• W3210659493 countsByYear W32106594932023 @default.
• W3210659493 crossrefType "journal-article" @default.
• W3210659493 hasAuthorship W3210659493A5030309010 @default.
• W3210659493 hasAuthorship W3210659493A5068864315 @default.
• W3210659493 hasAuthorship W3210659493A5077234337 @default.
• W3210659493 hasAuthorship W3210659493A5080582773 @default.
• W3210659493 hasAuthorship W3210659493A5086038672 @default.
• W3210659493 hasConcept C104317684 @default.
• W3210659493 hasConcept C126322002 @default.
• W3210659493 hasConcept C153911025 @default.
• W3210659493 hasConcept C17991360 @default.
• W3210659493 hasConcept C185592680 @default.
• W3210659493 hasConcept C190283241 @default.
• W3210659493 hasConcept C203014093 @default.
• W3210659493 hasConcept C2776415932 @default.
• W3210659493 hasConcept C2777730290 @default.
• W3210659493 hasConcept C2778754761 @default.
• W3210659493 hasConcept C2780472472 @default.
• W3210659493 hasConcept C502942594 @default.
• W3210659493 hasConcept C53227056 @default.
• W3210659493 hasConcept C553184892 @default.
• W3210659493 hasConcept C55493867 @default.
• W3210659493 hasConcept C71924100 @default.
• W3210659493 hasConcept C86803240 @default.
• W3210659493 hasConceptScore W3210659493C104317684 @default.
• W3210659493 hasConceptScore W3210659493C126322002 @default.
• W3210659493 hasConceptScore W3210659493C153911025 @default.
• W3210659493 hasConceptScore W3210659493C17991360 @default.
• W3210659493 hasConceptScore W3210659493C185592680 @default.
• W3210659493 hasConceptScore W3210659493C190283241 @default.
• W3210659493 hasConceptScore W3210659493C203014093 @default.
• W3210659493 hasConceptScore W3210659493C2776415932 @default.
• W3210659493 hasConceptScore W3210659493C2777730290 @default.
• W3210659493 hasConceptScore W3210659493C2778754761 @default.
• W3210659493 hasConceptScore W3210659493C2780472472 @default.
• W3210659493 hasConceptScore W3210659493C502942594 @default.
• W3210659493 hasConceptScore W3210659493C53227056 @default.

• next