Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210737565> ?p ?o ?g. }
- W3210737565 abstract "Abstract To preserve genome integrity, all living organisms have developed strategies to respond to chromosomal damage. One such response is the repair of DNA double-strand breaks (DSBs), one of the most toxic forms of DNA lesions. In E. coli , DSBs are repaired via the homologous recombination pathway, initiated by the RecBCD enzyme. RecBCD is essential for accurate chromosome maintenance but its over-expression can lead to reduced DNA repair ability. This apparent paradox suggests that RecBCD copy number may need to be tightly controlled within an optimal range. Using single-molecule fluo-rescence microscopy, we have established that RecB is present in very low abundance at mRNA and protein levels. RecB transcription shows high levels of fluctuations yet cell-to-cell protein variability remains remarkably low. We show that the post-transcriptional regulator Hfq binds to recBCD mRNAs and down-regulates RecB protein expression in vivo . Furthermore, when Hfq-mediated regulation is perturbed, we observe less effective noise reduction and reduced DNA repair capacity. Taken together, our results suggest a post-transcriptional regulatory mechanism where Hfq fine-tunes RecB expression by inhibiting RecB translation. This fine-tuning of RecB expression contributes to reducing noise in RecB protein expression and protects cells against the toxic consequences of too high RecBCD numbers." @default.
- W3210737565 created "2021-11-08" @default.
- W3210737565 creator A5009107922 @default.
- W3210737565 creator A5050840445 @default.
- W3210737565 creator A5061569924 @default.
- W3210737565 creator A5077613316 @default.
- W3210737565 date "2021-10-24" @default.
- W3210737565 modified "2023-09-27" @default.
- W3210737565 title "Fine-tuning of RecBCD expression by post-transcriptional regulation is required for optimal DNA repair in <i>Escherichia coli</i>" @default.
- W3210737565 cites W1503666504 @default.
- W3210737565 cites W1544663300 @default.
- W3210737565 cites W1600300375 @default.
- W3210737565 cites W1845702142 @default.
- W3210737565 cites W1922512116 @default.
- W3210737565 cites W1969512257 @default.
- W3210737565 cites W1969737118 @default.
- W3210737565 cites W1970663550 @default.
- W3210737565 cites W1971682695 @default.
- W3210737565 cites W1976009923 @default.
- W3210737565 cites W1979174978 @default.
- W3210737565 cites W1984916226 @default.
- W3210737565 cites W1989781175 @default.
- W3210737565 cites W1992189474 @default.
- W3210737565 cites W1996666978 @default.
- W3210737565 cites W1997990660 @default.
- W3210737565 cites W2018435536 @default.
- W3210737565 cites W2019050932 @default.
- W3210737565 cites W2019472515 @default.
- W3210737565 cites W2020596227 @default.
- W3210737565 cites W2025306938 @default.
- W3210737565 cites W2026738605 @default.
- W3210737565 cites W2037685552 @default.
- W3210737565 cites W2043198662 @default.
- W3210737565 cites W2053460598 @default.
- W3210737565 cites W2059053384 @default.
- W3210737565 cites W2062493030 @default.
- W3210737565 cites W2069957134 @default.
- W3210737565 cites W2071424950 @default.
- W3210737565 cites W2072888577 @default.
- W3210737565 cites W2075251971 @default.
- W3210737565 cites W2076353383 @default.
- W3210737565 cites W2079478900 @default.
- W3210737565 cites W2081225424 @default.
- W3210737565 cites W2089239776 @default.
- W3210737565 cites W2095999275 @default.
- W3210737565 cites W2096705138 @default.
- W3210737565 cites W2097851712 @default.
- W3210737565 cites W2101058845 @default.
- W3210737565 cites W2101703936 @default.
- W3210737565 cites W2102263295 @default.
- W3210737565 cites W2102789908 @default.
- W3210737565 cites W2107713942 @default.
- W3210737565 cites W2107810643 @default.
- W3210737565 cites W2108141605 @default.
- W3210737565 cites W2112456256 @default.
- W3210737565 cites W2113131574 @default.
- W3210737565 cites W2114870653 @default.
- W3210737565 cites W2115368299 @default.
- W3210737565 cites W2116137883 @default.
- W3210737565 cites W2119819241 @default.
- W3210737565 cites W2122121413 @default.
- W3210737565 cites W2125957234 @default.
- W3210737565 cites W2130487552 @default.
- W3210737565 cites W2137727595 @default.
- W3210737565 cites W2140886363 @default.
- W3210737565 cites W2141688036 @default.
- W3210737565 cites W2143325644 @default.
- W3210737565 cites W2144171940 @default.
- W3210737565 cites W2144447003 @default.
- W3210737565 cites W2145532900 @default.
- W3210737565 cites W2145767117 @default.
- W3210737565 cites W2147030152 @default.
- W3210737565 cites W2147256329 @default.
- W3210737565 cites W2148323109 @default.
- W3210737565 cites W2152392747 @default.
- W3210737565 cites W2155418451 @default.
- W3210737565 cites W2155516053 @default.
- W3210737565 cites W2157984621 @default.
- W3210737565 cites W2158473123 @default.
- W3210737565 cites W2159579373 @default.
- W3210737565 cites W2160084442 @default.
- W3210737565 cites W2161406501 @default.
- W3210737565 cites W2167244112 @default.
- W3210737565 cites W2169568690 @default.
- W3210737565 cites W2171280873 @default.
- W3210737565 cites W2215246329 @default.
- W3210737565 cites W2275928151 @default.
- W3210737565 cites W2315231855 @default.
- W3210737565 cites W2327011266 @default.
- W3210737565 cites W2329134896 @default.
- W3210737565 cites W2329524318 @default.
- W3210737565 cites W2330875406 @default.
- W3210737565 cites W2399953365 @default.
- W3210737565 cites W2498876603 @default.
- W3210737565 cites W2516278741 @default.
- W3210737565 cites W2528332035 @default.
- W3210737565 cites W2562944419 @default.
- W3210737565 cites W2744135688 @default.
- W3210737565 cites W2767434584 @default.
- W3210737565 cites W2777650842 @default.