Matches in SemOpenAlex for { <https://semopenalex.org/work/W3210917725> ?p ?o ?g. }
- W3210917725 abstract "The HIV-1 Nef and Vpu accessory proteins are known to protect infected cells from antibody-dependent cellular cytotoxicity (ADCC) responses by limiting exposure of CD4-induced (CD4i) envelope (Env) epitopes at the cell surface. Although both proteins target the host receptor CD4 for degradation, the extent of their functional redundancy is unknown. Here, we developed an intracellular staining technique that permits the intracellular detection of both Nef and Vpu in primary CD4+ T cells by flow cytometry. Using this method, we show that the combined expression of Nef and Vpu predicts the susceptibility of HIV-1-infected primary CD4+ T cells to ADCC by HIV+ plasma. We also show that Vpu cannot compensate for the absence of Nef, thus providing an explanation for why some infectious molecular clones that carry a LucR reporter gene upstream of Nef render infected cells more susceptible to ADCC responses. Our method thus represents a new tool to dissect the biological activity of Nef and Vpu in the context of other host and viral proteins within single infected CD4+ T cells." @default.
- W3210917725 created "2021-11-08" @default.
- W3210917725 creator A5000112132 @default.
- W3210917725 creator A5007481373 @default.
- W3210917725 creator A5014607507 @default.
- W3210917725 creator A5028344925 @default.
- W3210917725 creator A5032973683 @default.
- W3210917725 creator A5050888562 @default.
- W3210917725 creator A5059159168 @default.
- W3210917725 creator A5069313429 @default.
- W3210917725 creator A5075006919 @default.
- W3210917725 creator A5080069681 @default.
- W3210917725 date "2021-11-03" @default.
- W3210917725 modified "2023-10-14" @default.
- W3210917725 title "Detection of the HIV-1 accessory proteins Nef and Vpu by flow cytometry represents a new tool to study their functional interplay within a single infected CD4+ T cell" @default.
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