FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3211365402> ?p ?o ?g. }

• W3211365402 abstract "Radiotherapy is routinely used to combat glioblastoma (GBM). However, the treatment efficacy is often limited by the radioresistance of GBM cells.Two GBM lines MO59K and MO59J, differing in intrinsic radiosensitivity and mutational status of DNA-PK and ATM, were analyzed regarding their response to DNA-PK/PI3K/mTOR inhibition by PI-103 in combination with radiation. To this end we assessed colony-forming ability, induction and repair of DNA damage by γH2AX and 53BP1, expression of marker proteins, including those belonging to NHEJ and HR repair pathways, degree of apoptosis, autophagy, and cell cycle alterations.We found that PI-103 radiosensitized MO59K cells but, surprisingly, it induced radiation resistance in MO59J cells. Treatment of MO59K cells with PI-103 lead to protraction of the DNA damage repair as compared to drug-free irradiated cells. In PI-103-treated and irradiated MO59J cells the foci numbers of both proteins was higher than in the drug-free samples, but a large portion of DNA damage was quickly repaired. Another cell line-specific difference includes diminished expression of p53 in MO59J cells, which was further reduced by PI-103. Additionally, PI-103-treated MO59K cells exhibited an increased expression of the apoptosis marker cleaved PARP and increased subG1 fraction. Moreover, irradiation induced a strong G2 arrest in MO59J cells (~ 80% vs. ~ 50% in MO59K), which was, however, partially reduced in the presence of PI-103. In contrast, treatment with PI-103 increased the G2 fraction in irradiated MO59K cells.The triple-target inhibitor PI-103 exerted radiosensitization on MO59K cells, but, unexpectedly, caused radioresistance in the MO59J line, lacking DNA-PK. The difference is most likely due to low expression of the DNA-PK substrate p53 in MO59J cells, which was further reduced by PI-103. This led to less apoptosis as compared to drug-free MO59J cells and enhanced survival via partially abolished cell-cycle arrest. The findings suggest that the lack of DNA-PK-dependent NHEJ in MO59J line might be compensated by DNA-PK independent DSB repair via a yet unknown mechanism." @default.
• W3211365402 created "2021-11-22" @default.
• W3211365402 creator A5009855159 @default.
• W3211365402 creator A5017407947 @default.
• W3211365402 creator A5019572077 @default.
• W3211365402 creator A5046756579 @default.
• W3211365402 creator A5054433502 @default.
• W3211365402 creator A5060229095 @default.
• W3211365402 creator A5079910911 @default.
• W3211365402 creator A5091806326 @default.
• W3211365402 date "2021-11-11" @default.
• W3211365402 modified "2023-09-30" @default.
• W3211365402 title "Opposite effects of the triple target (DNA-PK/PI3K/mTOR) inhibitor PI-103 on the radiation sensitivity of glioblastoma cell lines proficient and deficient in DNA-PKcs" @default.
• W3211365402 cites W1529935140 @default.
• W3211365402 cites W1554069754 @default.
• W3211365402 cites W1593937083 @default.
• W3211365402 cites W1620981049 @default.
• W3211365402 cites W1644206514 @default.
• W3211365402 cites W1908696053 @default.
• W3211365402 cites W1977267072 @default.
• W3211365402 cites W1977798765 @default.
• W3211365402 cites W1985775217 @default.
• W3211365402 cites W1999998017 @default.
• W3211365402 cites W2003167607 @default.
• W3211365402 cites W2015176250 @default.
• W3211365402 cites W2015181794 @default.
• W3211365402 cites W2033148121 @default.
• W3211365402 cites W2038451572 @default.
• W3211365402 cites W2038961829 @default.
• W3211365402 cites W2047758510 @default.
• W3211365402 cites W2050573392 @default.
• W3211365402 cites W2052801384 @default.
• W3211365402 cites W2061019285 @default.
• W3211365402 cites W2061414735 @default.
• W3211365402 cites W2067228541 @default.
• W3211365402 cites W2071277764 @default.
• W3211365402 cites W2072375912 @default.
• W3211365402 cites W2075092060 @default.
• W3211365402 cites W2076544924 @default.
• W3211365402 cites W2080484066 @default.
• W3211365402 cites W2081127556 @default.
• W3211365402 cites W2087667264 @default.
• W3211365402 cites W2091545748 @default.
• W3211365402 cites W2096287682 @default.
• W3211365402 cites W2096439168 @default.
• W3211365402 cites W2101209845 @default.
• W3211365402 cites W2101579411 @default.
• W3211365402 cites W2110443482 @default.
• W3211365402 cites W2114069643 @default.
• W3211365402 cites W2130308369 @default.
• W3211365402 cites W2131674185 @default.
• W3211365402 cites W2133481253 @default.
• W3211365402 cites W2133540186 @default.
• W3211365402 cites W2141125222 @default.
• W3211365402 cites W2143629217 @default.
• W3211365402 cites W2144169031 @default.
• W3211365402 cites W2149624733 @default.
• W3211365402 cites W2152472586 @default.
• W3211365402 cites W2152525494 @default.
• W3211365402 cites W2157005154 @default.
• W3211365402 cites W2288903023 @default.
• W3211365402 cites W2395480421 @default.
• W3211365402 cites W2410482852 @default.
• W3211365402 cites W2411753014 @default.
• W3211365402 cites W2473989551 @default.
• W3211365402 cites W2808162513 @default.
• W3211365402 cites W2905395632 @default.
• W3211365402 cites W3003690943 @default.
• W3211365402 cites W3043417644 @default.
• W3211365402 cites W65598924 @default.
• W3211365402 doi "https://doi.org/10.1186/s12885-021-08930-1" @default.
• W3211365402 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8582108" @default.
• W3211365402 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34763650" @default.
• W3211365402 hasPublicationYear "2021" @default.
• W3211365402 type Work @default.
• W3211365402 sameAs 3211365402 @default.
• W3211365402 citedByCount "6" @default.
• W3211365402 countsByYear W32113654022022 @default.
• W3211365402 countsByYear W32113654022023 @default.
• W3211365402 crossrefType "journal-article" @default.
• W3211365402 hasAuthorship W3211365402A5009855159 @default.
• W3211365402 hasAuthorship W3211365402A5017407947 @default.
• W3211365402 hasAuthorship W3211365402A5019572077 @default.
• W3211365402 hasAuthorship W3211365402A5046756579 @default.
• W3211365402 hasAuthorship W3211365402A5054433502 @default.
• W3211365402 hasAuthorship W3211365402A5060229095 @default.
• W3211365402 hasAuthorship W3211365402A5079910911 @default.
• W3211365402 hasAuthorship W3211365402A5091806326 @default.
• W3211365402 hasBestOaLocation W32113654023 @default.
• W3211365402 hasConcept C111337013 @default.
• W3211365402 hasConcept C121332964 @default.
• W3211365402 hasConcept C126322002 @default.
• W3211365402 hasConcept C134935766 @default.
• W3211365402 hasConcept C143425029 @default.
• W3211365402 hasConcept C153911025 @default.
• W3211365402 hasConcept C185544564 @default.
• W3211365402 hasConcept C190283241 @default.
• W3211365402 hasConcept C206684579 @default.
• W3211365402 hasConcept C26114605 @default.
• W3211365402 hasConcept C29537977 @default.

• next