Matches in SemOpenAlex for { <https://semopenalex.org/work/W3211404966> ?p ?o ?g. }
- W3211404966 abstract "The early insect embryo develops as a multinucleated cell distributing the genome uniformly to the cell cortex. Mechanistic insight for nuclear positioning beyond cytoskeletal requirements is missing. Contemporary hypotheses propose actomyosin-driven cytoplasmic movement transporting nuclei or repulsion of neighbor nuclei driven by microtubule motors. Here, we show that microtubule cross-linking by Feo and Klp3A is essential for nuclear distribution and internuclear distance maintenance in Drosophila. Germline knockdown causes irregular, less-dense nuclear delivery to the cell cortex and smaller distribution in ex vivo embryo explants. A minimal internuclear distance is maintained in explants from control embryos but not from Feo-inhibited embryos, following micromanipulation-assisted repositioning. A dimerization-deficient Feo abolishes nuclear separation in embryo explants, while the full-length protein rescues the genetic knockdown. We conclude that Feo and Klp3A cross-linking of antiparallel microtubule overlap generates a length-regulated mechanical link between neighboring microtubule asters. Enabled by a novel experimental approach, our study illuminates an essential process of embryonic multicellularity." @default.
- W3211404966 created "2021-11-22" @default.
- W3211404966 creator A5026429369 @default.
- W3211404966 creator A5032444078 @default.
- W3211404966 creator A5073948115 @default.
- W3211404966 creator A5074001643 @default.
- W3211404966 date "2021-11-12" @default.
- W3211404966 modified "2023-10-18" @default.
- W3211404966 title "Astral microtubule cross-linking safeguards uniform nuclear distribution in the <i>Drosophila</i> syncytium" @default.
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- W3211404966 doi "https://doi.org/10.1083/jcb.202007209" @default.
- W3211404966 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8594625" @default.
- W3211404966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34766978" @default.