Matches in SemOpenAlex for { <https://semopenalex.org/work/W3212084611> ?p ?o ?g. }
- W3212084611 endingPage "102978" @default.
- W3212084611 startingPage "102978" @default.
- W3212084611 abstract "Most of the methods used today for investigation of nanoparticle binding to living cells imply a quantitative or qualitative analysis and do not provide information on the binding dynamics. To meet the challenge, we show here for the first time that the interaction of metal nanoparticles with eukaryotic cells in the flow system in dynamics can be studied by the method of multiparametric surface plasmon resonance. To this aim, we obtained a spectrum of colloidally stable targeted magnetite nanoparticles modified by phytolectins (SBA, WGA, ConA) of different specificity to monosaccharides (GalNAc, GlcNAc, and Man, respectively), and studied the interaction of these conjugates with human epidermoid carcinoma A431 cells. We showed that multiparametric surface plasmon resonance can be effectively used as a label-free method to study the process of dynamic mass transfer in the nanoparticle*cell system in the fluid cell. We show that not only the commonly used parameter in such systems, the angle of the minimum peak, θSPR, at the full angular spectrum, but also the intensity, I, of this peak, can be used to study the binding of targeted nanoparticles with living cells in dynamics. This is due to the contribution of metal nanoparticles to resonant absorption of incident electromagnetic radiation by free electrons at the interface between media with different refractive indices. To get the most relevant quantitative data on nanoparticle binding to the cell surface, we combined this assay with our original MPQ-cytometry method (Magnetic Particle Quantification based cytometry) precisely quantifying the nanoparticles in the static cell sample. By the combination of label-free plasmonic and magnetometry-based MPQ-cytometry methods, we showed that nanoparticles modified with agglutinin from soybeans, SBA, most efficiently (up to 4.2 ± 0.1 pg/cell) bind to epidermoid carcinoma cells with achieving a saturation at 12 min" @default.
- W3212084611 created "2021-11-22" @default.
- W3212084611 creator A5055501415 @default.
- W3212084611 creator A5058335791 @default.
- W3212084611 creator A5066621253 @default.
- W3212084611 creator A5090597738 @default.
- W3212084611 date "2021-12-01" @default.
- W3212084611 modified "2023-09-27" @default.
- W3212084611 title "Label-free methods of multiparametric surface plasmon resonance and MPQ-cytometry for quantitative real-time measurements of targeted magnetic nanoparticles complexation with living cancer cells" @default.
- W3212084611 cites W1508895967 @default.
- W3212084611 cites W1963595841 @default.
- W3212084611 cites W1985589802 @default.
- W3212084611 cites W2005196200 @default.
- W3212084611 cites W2005246945 @default.
- W3212084611 cites W2018159558 @default.
- W3212084611 cites W2020520614 @default.
- W3212084611 cites W2023268526 @default.
- W3212084611 cites W2044838811 @default.
- W3212084611 cites W2056150471 @default.
- W3212084611 cites W2059201273 @default.
- W3212084611 cites W2063013932 @default.
- W3212084611 cites W2072430760 @default.
- W3212084611 cites W2076853819 @default.
- W3212084611 cites W2085977990 @default.
- W3212084611 cites W2090345981 @default.
- W3212084611 cites W2108092944 @default.
- W3212084611 cites W2133238031 @default.
- W3212084611 cites W2140782011 @default.
- W3212084611 cites W2171633565 @default.
- W3212084611 cites W2290383875 @default.
- W3212084611 cites W2397694777 @default.
- W3212084611 cites W2484794086 @default.
- W3212084611 cites W2518751911 @default.
- W3212084611 cites W2525123034 @default.
- W3212084611 cites W2615629757 @default.
- W3212084611 cites W2626045638 @default.
- W3212084611 cites W2771265151 @default.
- W3212084611 cites W2776931993 @default.
- W3212084611 cites W2784289535 @default.
- W3212084611 cites W2788018204 @default.
- W3212084611 cites W2802976465 @default.
- W3212084611 cites W2889033683 @default.
- W3212084611 cites W2891391322 @default.
- W3212084611 cites W2891892762 @default.
- W3212084611 cites W2905653672 @default.
- W3212084611 cites W2935892452 @default.
- W3212084611 cites W2936612019 @default.
- W3212084611 cites W2946630278 @default.
- W3212084611 cites W2956427584 @default.
- W3212084611 cites W2973014626 @default.
- W3212084611 cites W3037506784 @default.
- W3212084611 cites W3040142808 @default.
- W3212084611 cites W3043880866 @default.
- W3212084611 cites W3083803591 @default.
- W3212084611 cites W3089086388 @default.
- W3212084611 cites W3168483671 @default.
- W3212084611 cites W3176581805 @default.
- W3212084611 doi "https://doi.org/10.1016/j.mtcomm.2021.102978" @default.
- W3212084611 hasPublicationYear "2021" @default.
- W3212084611 type Work @default.
- W3212084611 sameAs 3212084611 @default.
- W3212084611 citedByCount "7" @default.
- W3212084611 countsByYear W32120846112021 @default.
- W3212084611 countsByYear W32120846112022 @default.
- W3212084611 crossrefType "journal-article" @default.
- W3212084611 hasAuthorship W3212084611A5055501415 @default.
- W3212084611 hasAuthorship W3212084611A5058335791 @default.
- W3212084611 hasAuthorship W3212084611A5066621253 @default.
- W3212084611 hasAuthorship W3212084611A5090597738 @default.
- W3212084611 hasConcept C106847996 @default.
- W3212084611 hasConcept C113196181 @default.
- W3212084611 hasConcept C124657808 @default.
- W3212084611 hasConcept C12554922 @default.
- W3212084611 hasConcept C153911025 @default.
- W3212084611 hasConcept C155672457 @default.
- W3212084611 hasConcept C171250308 @default.
- W3212084611 hasConcept C185592680 @default.
- W3212084611 hasConcept C192562407 @default.
- W3212084611 hasConcept C43617362 @default.
- W3212084611 hasConcept C553184892 @default.
- W3212084611 hasConcept C86803240 @default.
- W3212084611 hasConceptScore W3212084611C106847996 @default.
- W3212084611 hasConceptScore W3212084611C113196181 @default.
- W3212084611 hasConceptScore W3212084611C124657808 @default.
- W3212084611 hasConceptScore W3212084611C12554922 @default.
- W3212084611 hasConceptScore W3212084611C153911025 @default.
- W3212084611 hasConceptScore W3212084611C155672457 @default.
- W3212084611 hasConceptScore W3212084611C171250308 @default.
- W3212084611 hasConceptScore W3212084611C185592680 @default.
- W3212084611 hasConceptScore W3212084611C192562407 @default.
- W3212084611 hasConceptScore W3212084611C43617362 @default.
- W3212084611 hasConceptScore W3212084611C553184892 @default.
- W3212084611 hasConceptScore W3212084611C86803240 @default.
- W3212084611 hasFunder F4320321912 @default.
- W3212084611 hasLocation W32120846111 @default.
- W3212084611 hasOpenAccess W3212084611 @default.
- W3212084611 hasPrimaryLocation W32120846111 @default.
- W3212084611 hasRelatedWork W1989746838 @default.