FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3212456861> ?p ?o ?g. }

• W3212456861 endingPage "359" @default.
• W3212456861 startingPage "359" @default.
• W3212456861 abstract "Spermiogenesis is a complex and tightly regulated process, consisting of acrosomal biogenesis, condensation of chromatin, flagellar assembly, and disposal of extra cytoplasm. Previous studies have reported that sperm flagellar 2 (SPEF2) deficiency causes severe asthenoteratozoospermia owing to spermiogenesis failure, but the underlying molecular mechanism in humans remains unclear. Here, we performed proteomic analysis on spermatozoa from three SPEF2 mutant patients to study the functional role of SPEF2 during sperm tail development. A total of 1262 differentially expressed proteins were detected, including 486 upregulated and 776 downregulated. The constructed heat map of the differentially expressed proteins showed similar trends. Among these, the expression of proteins related to flagellar assembly, including SPEF2, sperm associated antigen 6 (SPAG6), dynein light chain tctex-type 1 (DYNLT1), radial spoke head component 1 (RSPH1), translocase of outer mitochondrial membrane 20 (TOM20), EF-hand domain containing 1 (EFHC1), meiosis-specific nuclear structural 1 (MNS1) and intraflagellar transport 20 (IFT20), was verified by western blot. Functional clustering analysis indicated that these differentially expressed proteins were specifically enriched for terms such as spermatid development and flagellar assembly. Furthermore, we showed that SPEF2 interacts with radial spoke head component 9 (RSPH9) and IFT20 in vitro, which are well-studied components of radial spokes or intra-flagellar transport and are essential for flagellar assembly. These results provide a rich resource for further investigation into the molecular mechanism underlying the role that SPEF2 plays in sperm tail development and could provide a theoretical basis for gene therapy in SPEF2 mutant patients in the future." @default.
• W3212456861 created "2021-11-22" @default.
• W3212456861 creator A5000026105 @default.
• W3212456861 creator A5000873086 @default.
• W3212456861 creator A5004909924 @default.
• W3212456861 creator A5032300551 @default.
• W3212456861 creator A5047903714 @default.
• W3212456861 creator A5050900684 @default.
• W3212456861 creator A5053957782 @default.
• W3212456861 creator A5074544812 @default.
• W3212456861 creator A5082886409 @default.
• W3212456861 date "2022-01-01" @default.
• W3212456861 modified "2023-10-01" @default.
• W3212456861 title "Sperm flagellar 2 (SPEF2) is essential for sperm flagellar assembly in humans" @default.
• W3212456861 cites W1809267070 @default.
• W3212456861 cites W1973632178 @default.
• W3212456861 cites W2003065118 @default.
• W3212456861 cites W2010366657 @default.
• W3212456861 cites W2015120218 @default.
• W3212456861 cites W2016188546 @default.
• W3212456861 cites W2018698545 @default.
• W3212456861 cites W2039186803 @default.
• W3212456861 cites W2092077315 @default.
• W3212456861 cites W2108946743 @default.
• W3212456861 cites W2115042236 @default.
• W3212456861 cites W2115536632 @default.
• W3212456861 cites W2121667153 @default.
• W3212456861 cites W2126320768 @default.
• W3212456861 cites W2126879318 @default.
• W3212456861 cites W2131521136 @default.
• W3212456861 cites W2145506897 @default.
• W3212456861 cites W2150787212 @default.
• W3212456861 cites W2156400902 @default.
• W3212456861 cites W2277698104 @default.
• W3212456861 cites W2326112268 @default.
• W3212456861 cites W2592813833 @default.
• W3212456861 cites W2606744545 @default.
• W3212456861 cites W2617398306 @default.
• W3212456861 cites W2625149483 @default.
• W3212456861 cites W2752455371 @default.
• W3212456861 cites W2806261901 @default.
• W3212456861 cites W2885166646 @default.
• W3212456861 cites W2913732601 @default.
• W3212456861 cites W2914909947 @default.
• W3212456861 cites W2943756277 @default.
• W3212456861 cites W2948019784 @default.
• W3212456861 cites W2955895400 @default.
• W3212456861 cites W2973155278 @default.
• W3212456861 cites W2976838777 @default.
• W3212456861 cites W2998887378 @default.
• W3212456861 cites W3038657332 @default.
• W3212456861 cites W3048729698 @default.
• W3212456861 cites W3090620689 @default.
• W3212456861 cites W3123411243 @default.
• W3212456861 doi "https://doi.org/10.4103/aja202154" @default.
• W3212456861 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34755699" @default.
• W3212456861 hasPublicationYear "2022" @default.
• W3212456861 type Work @default.
• W3212456861 sameAs 3212456861 @default.
• W3212456861 citedByCount "9" @default.
• W3212456861 countsByYear W32124568612022 @default.
• W3212456861 countsByYear W32124568612023 @default.
• W3212456861 crossrefType "journal-article" @default.
• W3212456861 hasAuthorship W3212456861A5000026105 @default.
• W3212456861 hasAuthorship W3212456861A5000873086 @default.
• W3212456861 hasAuthorship W3212456861A5004909924 @default.
• W3212456861 hasAuthorship W3212456861A5032300551 @default.
• W3212456861 hasAuthorship W3212456861A5047903714 @default.
• W3212456861 hasAuthorship W3212456861A5050900684 @default.
• W3212456861 hasAuthorship W3212456861A5053957782 @default.
• W3212456861 hasAuthorship W3212456861A5074544812 @default.
• W3212456861 hasAuthorship W3212456861A5082886409 @default.
• W3212456861 hasBestOaLocation W32124568611 @default.
• W3212456861 hasConcept C104317684 @default.
• W3212456861 hasConcept C106987784 @default.
• W3212456861 hasConcept C115811362 @default.
• W3212456861 hasConcept C20418707 @default.
• W3212456861 hasConcept C2777394357 @default.
• W3212456861 hasConcept C2778619154 @default.
• W3212456861 hasConcept C2781087480 @default.
• W3212456861 hasConcept C37590093 @default.
• W3212456861 hasConcept C54355233 @default.
• W3212456861 hasConcept C86803240 @default.
• W3212456861 hasConcept C95444343 @default.
• W3212456861 hasConceptScore W3212456861C104317684 @default.
• W3212456861 hasConceptScore W3212456861C106987784 @default.
• W3212456861 hasConceptScore W3212456861C115811362 @default.
• W3212456861 hasConceptScore W3212456861C20418707 @default.
• W3212456861 hasConceptScore W3212456861C2777394357 @default.
• W3212456861 hasConceptScore W3212456861C2778619154 @default.
• W3212456861 hasConceptScore W3212456861C2781087480 @default.
• W3212456861 hasConceptScore W3212456861C37590093 @default.
• W3212456861 hasConceptScore W3212456861C54355233 @default.
• W3212456861 hasConceptScore W3212456861C86803240 @default.
• W3212456861 hasConceptScore W3212456861C95444343 @default.
• W3212456861 hasIssue "4" @default.
• W3212456861 hasLocation W32124568611 @default.
• W3212456861 hasLocation W32124568612 @default.

• next