Matches in SemOpenAlex for { <https://semopenalex.org/work/W3212463640> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W3212463640 abstract "<h3>Introduction</h3> Mismatch Repair (MMR) deficient colorectal cancers are an important cohort to study as these patients have a high neo-epitope load, suggesting they may respond well to immunotherapy. We previously reported deficient MMR due to loss of MLH1 expression occurs in >20% of all inflammatory bowel disease-associated colorectal cancers (IBD-CRC). Manually identifying MHL1 deficient tumours from immunostained samples is time-consuming and labour-intensive. Therefore, we aimed to train QuPath, a digital image analysis program, to accurately identify MLH1 deficient IBD-CRCs from a tissue microarray (TMA) containing normal colon and IBD-CRC. <h3>Methods</h3> A TMA (n=162 cores) containing normal colon and IBD-CRC was immunostained for MLH1, digitalised and imported into QuPath. A representative sample (14 sections of 500μm x 500μm) was used to train a QuPath algorithm to identify tissue histology (normal epithelium, tumour, immune infiltrate or stroma) and MLH1 expression pattern. The algorithm was applied to the whole TMA. Accuracy level for histology and MLH1 classification was set at >75% of cells per category; cores where neither category met this threshold were flagged for review and these cores were histopathologically reviewed qualitatively. Data were exported to IBM®SPSS® (V25.0) for statistical analysis. <h3>Results</h3> Tissue histology and MLH1 expression was correctly identified in 87/113 (77%) cases, misclassified in 1/113 (0.9%) case and flagged for manual review in 25/113 (22.1%) cases. Reasons cores were flagged for review included: small quantity of tissue, atypical morphology, excessive immune infiltrate, normal epithelium and patchy immunostaining. Of cores not flagged for review (n=88), the algorithm was highly sensitive (100%) and specific (98.6%) for identifying MLH1-deficient IBD-CRC, κ=0.967 (95%CI 0.902-1.032) (p<0.001). <h3>Conclusions</h3> MLH1 deficient IBD-CRC can be accurately identified using our QuPath algorithm, with an acceptable proportion of equivocal samples highlighted for manual evaluation. This process could be efficiently automated in conventional NHS IBD-CRC surveillance and treatment programmes to examine all colonic tissue and tumour specimens for MLH1 expression, thus identifying patients who may respond well to immunotherapy. This approach now needs to be validated using endoscopic biopsies." @default.
- W3212463640 created "2021-11-22" @default.
- W3212463640 creator A5007750313 @default.
- W3212463640 creator A5019378167 @default.
- W3212463640 creator A5032672820 @default.
- W3212463640 creator A5054505979 @default.
- W3212463640 creator A5055039884 @default.
- W3212463640 date "2021-11-01" @default.
- W3212463640 modified "2023-10-01" @default.
- W3212463640 title "ATU-10 QuPath machine learning algorithm accurately identifies MLH1 deficient inflammatory bowel disease-associated colorectal cancer" @default.
- W3212463640 doi "https://doi.org/10.1136/gutjnl-2021-bsg.43" @default.
- W3212463640 hasPublicationYear "2021" @default.
- W3212463640 type Work @default.
- W3212463640 sameAs 3212463640 @default.
- W3212463640 citedByCount "0" @default.
- W3212463640 crossrefType "proceedings-article" @default.
- W3212463640 hasAuthorship W3212463640A5007750313 @default.
- W3212463640 hasAuthorship W3212463640A5019378167 @default.
- W3212463640 hasAuthorship W3212463640A5032672820 @default.
- W3212463640 hasAuthorship W3212463640A5054505979 @default.
- W3212463640 hasAuthorship W3212463640A5055039884 @default.
- W3212463640 hasBestOaLocation W32124636401 @default.
- W3212463640 hasConcept C111425858 @default.
- W3212463640 hasConcept C11413529 @default.
- W3212463640 hasConcept C121608353 @default.
- W3212463640 hasConcept C126322002 @default.
- W3212463640 hasConcept C142724271 @default.
- W3212463640 hasConcept C143998085 @default.
- W3212463640 hasConcept C193270364 @default.
- W3212463640 hasConcept C204232928 @default.
- W3212463640 hasConcept C2777547418 @default.
- W3212463640 hasConcept C2778260677 @default.
- W3212463640 hasConcept C2779134260 @default.
- W3212463640 hasConcept C41008148 @default.
- W3212463640 hasConcept C526805850 @default.
- W3212463640 hasConcept C57742111 @default.
- W3212463640 hasConcept C60748783 @default.
- W3212463640 hasConcept C71924100 @default.
- W3212463640 hasConceptScore W3212463640C111425858 @default.
- W3212463640 hasConceptScore W3212463640C11413529 @default.
- W3212463640 hasConceptScore W3212463640C121608353 @default.
- W3212463640 hasConceptScore W3212463640C126322002 @default.
- W3212463640 hasConceptScore W3212463640C142724271 @default.
- W3212463640 hasConceptScore W3212463640C143998085 @default.
- W3212463640 hasConceptScore W3212463640C193270364 @default.
- W3212463640 hasConceptScore W3212463640C204232928 @default.
- W3212463640 hasConceptScore W3212463640C2777547418 @default.
- W3212463640 hasConceptScore W3212463640C2778260677 @default.
- W3212463640 hasConceptScore W3212463640C2779134260 @default.
- W3212463640 hasConceptScore W3212463640C41008148 @default.
- W3212463640 hasConceptScore W3212463640C526805850 @default.
- W3212463640 hasConceptScore W3212463640C57742111 @default.
- W3212463640 hasConceptScore W3212463640C60748783 @default.
- W3212463640 hasConceptScore W3212463640C71924100 @default.
- W3212463640 hasLocation W32124636401 @default.
- W3212463640 hasOpenAccess W3212463640 @default.
- W3212463640 hasPrimaryLocation W32124636401 @default.
- W3212463640 hasRelatedWork W2073792004 @default.
- W3212463640 hasRelatedWork W2075411465 @default.
- W3212463640 hasRelatedWork W2105306738 @default.
- W3212463640 hasRelatedWork W2106980433 @default.
- W3212463640 hasRelatedWork W2155293355 @default.
- W3212463640 hasRelatedWork W2318988265 @default.
- W3212463640 hasRelatedWork W2358565899 @default.
- W3212463640 hasRelatedWork W2408693058 @default.
- W3212463640 hasRelatedWork W2910298919 @default.
- W3212463640 hasRelatedWork W4327724894 @default.
- W3212463640 isParatext "false" @default.
- W3212463640 isRetracted "false" @default.
- W3212463640 magId "3212463640" @default.
- W3212463640 workType "article" @default.