Matches in SemOpenAlex for { <https://semopenalex.org/work/W3212662463> ?p ?o ?g. }
- W3212662463 abstract "ABSTRACT Human breast milk is a dynamic fluid that contains millions of cells, but their identities and phenotypic properties are poorly understood. We used single-cell RNA-seq (scRNA-seq) to characterize the transcriptomes of cells from human breast milk (hBM) across lactational time from 3 to 632 days postpartum in 15 donors. We find that the majority of cells in human breast milk are lactocytes, a specialized epithelial subset, and cell type frequencies shift over the course of lactation yielding greater epithelial diversity at later points. Analysis of lactocytes reveals a continuum of cell states characterized by transcriptional changes in hormone, growth factor, and milk production related pathways. Generalized additive models suggest that one sub-cluster, LALBA low epithelial cells, increase as a function of time postpartum, daycare attendance, and the use of hormonal birth control. We identify several sub-clusters of macrophages in hBM that are enriched for tolerogenic functions, possibly playing a role in protecting the mammary gland during lactation. Our description of the cellular components of breast milk, their association with maternal-infant dyad metadata and quantification of alterations at the gene and pathways levels provides the first detailed longitudinal picture of human breast milk cells across lactational time. This work paves the way for future investigations of how a potential division of cellular labor and differential hormone regulation might be leveraged therapeutically to support healthy lactation and potentially aid in milk production." @default.
- W3212662463 created "2021-11-22" @default.
- W3212662463 creator A5002591108 @default.
- W3212662463 creator A5008374226 @default.
- W3212662463 creator A5010265776 @default.
- W3212662463 creator A5040134285 @default.
- W3212662463 creator A5042316021 @default.
- W3212662463 creator A5043637166 @default.
- W3212662463 creator A5044078921 @default.
- W3212662463 creator A5048353499 @default.
- W3212662463 creator A5071369153 @default.
- W3212662463 creator A5074269926 @default.
- W3212662463 creator A5082982116 @default.
- W3212662463 creator A5083743078 @default.
- W3212662463 creator A5086687499 @default.
- W3212662463 date "2021-11-14" @default.
- W3212662463 modified "2023-10-17" @default.
- W3212662463 title "Cellular and transcriptional diversity over the course of human lactation" @default.
- W3212662463 cites W1176278669 @default.
- W3212662463 cites W132416300 @default.
- W3212662463 cites W1486018587 @default.
- W3212662463 cites W1512335343 @default.
- W3212662463 cites W1542090274 @default.
- W3212662463 cites W1745675679 @default.
- W3212662463 cites W1828150019 @default.
- W3212662463 cites W1888924204 @default.
- W3212662463 cites W1923917571 @default.
- W3212662463 cites W1927705775 @default.
- W3212662463 cites W1974860236 @default.
- W3212662463 cites W1978832968 @default.
- W3212662463 cites W2000793566 @default.
- W3212662463 cites W2001392617 @default.
- W3212662463 cites W2001708910 @default.
- W3212662463 cites W2002302708 @default.
- W3212662463 cites W2011033752 @default.
- W3212662463 cites W2015582204 @default.
- W3212662463 cites W2018297306 @default.
- W3212662463 cites W2023356051 @default.
- W3212662463 cites W2028207904 @default.
- W3212662463 cites W2035588494 @default.
- W3212662463 cites W2036954935 @default.
- W3212662463 cites W2041672503 @default.
- W3212662463 cites W2071833891 @default.
- W3212662463 cites W2080597582 @default.
- W3212662463 cites W2085263701 @default.
- W3212662463 cites W2085769077 @default.
- W3212662463 cites W2085832350 @default.
- W3212662463 cites W2090203203 @default.
- W3212662463 cites W2103017472 @default.
- W3212662463 cites W2117468155 @default.
- W3212662463 cites W2119160928 @default.
- W3212662463 cites W2128534845 @default.
- W3212662463 cites W2128902257 @default.
- W3212662463 cites W2140482385 @default.
- W3212662463 cites W2147028876 @default.
- W3212662463 cites W2149300436 @default.
- W3212662463 cites W2151771969 @default.
- W3212662463 cites W2169691459 @default.
- W3212662463 cites W2179438025 @default.
- W3212662463 cites W2267159071 @default.
- W3212662463 cites W2276518561 @default.
- W3212662463 cites W2283848729 @default.
- W3212662463 cites W2313144233 @default.
- W3212662463 cites W2346603537 @default.
- W3212662463 cites W2410729773 @default.
- W3212662463 cites W2605907314 @default.
- W3212662463 cites W2735900593 @default.
- W3212662463 cites W2765283470 @default.
- W3212662463 cites W2771538763 @default.
- W3212662463 cites W2788593837 @default.
- W3212662463 cites W2800392236 @default.
- W3212662463 cites W2803144214 @default.
- W3212662463 cites W2811299341 @default.
- W3212662463 cites W2817838603 @default.
- W3212662463 cites W2886230523 @default.
- W3212662463 cites W2888169023 @default.
- W3212662463 cites W2898764631 @default.
- W3212662463 cites W2921479990 @default.
- W3212662463 cites W2924885918 @default.
- W3212662463 cites W2949177718 @default.
- W3212662463 cites W2951638683 @default.
- W3212662463 cites W2954731681 @default.
- W3212662463 cites W2962785200 @default.
- W3212662463 cites W2967736887 @default.
- W3212662463 cites W2975143648 @default.
- W3212662463 cites W2980870655 @default.
- W3212662463 cites W2989807288 @default.
- W3212662463 cites W3004565112 @default.
- W3212662463 cites W3012822000 @default.
- W3212662463 cites W3020633324 @default.
- W3212662463 cites W3032687904 @default.
- W3212662463 cites W3041793655 @default.
- W3212662463 cites W3047028171 @default.
- W3212662463 cites W3082920786 @default.
- W3212662463 cites W3093368337 @default.
- W3212662463 cites W3093821873 @default.
- W3212662463 cites W3095524886 @default.
- W3212662463 cites W3103381170 @default.
- W3212662463 cites W3104716179 @default.
- W3212662463 cites W3106212850 @default.