FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3212784225> ?p ?o ?g. }

• W3212784225 endingPage "110351" @default.
• W3212784225 startingPage "110351" @default.
• W3212784225 abstract "Allergen-specific immunotherapy (AIT) constitutes the only curative approach for allergy treatment. There is need for improvement of AIT in veterinary medicine, such as in horses suffering from insect bite hypersensitivity, an IgE-mediated dermatitis to Culicoides. Dendritic cell (DC)-targeting represents an efficient method to increase antigen immunogenicity. It is studied primarily for its use in improvement of cancer therapy and vaccines, but may also be useful for improving AIT efficacy. Immunomodulators, like the Toll-like receptor 4 (TLR-4) agonist monophosphoryl lipid-A (MPLA) has been shown to enhance the IL-10 response in horses, while CpG-rich oligonucleotides (CpG-ODN), acting as TLR-9 agonists, have been shown to induce Th1 or regulatory responses in horses with equine asthma. Our aim was to evaluate in vitro effects of antigen-targeting to equine DC with an antigen-fused peptide known to target human and mouse DC and investigate whether addition of MPLA or CpG-ODN would further improve the induced immune response with regard to finding optimal conditions for equine AIT. For this purpose, DC-binding peptides were fused to the model antigen ovalbumin (OVA) and to the recombinant Culicoides allergen Cul o3. Effects of DC-binding peptides on cellular antigen uptake and induction of T cell proliferation were assessed. Polarity of the immune response was analysed by quantifying IFN-γ, IL-4, IL-10, IL-17 and IFN-α in supernatants of antigen-stimulated peripheral blood mononuclear cells (PBMC) in presence or absence of adjuvants. Fusion of DC-binding peptides to OVA significantly enhanced antigen-uptake by equine DC. DC primed with DC-binding peptides coupled to OVA or Cul o3 induced a significantly higher T-cell proliferation compared to the corresponding control antigens. PBMC stimulation with DC-binding peptides coupled to Cul o3 elicited a significant increase in the pro-inflammatory cytokines IFN-γ, IL-4, IL-17, as well as the anti-inflammatory IL-10, but not of IFN-α. Adjuvant addition further enhanced the effect of the DC-binding peptides by significantly increasing the production of IFN-γ, IL-4, IL-10 and IFN-α (CpG-ODN) and IL-10 (MPLA), while simultaneously suppressing IFN-γ, IL-4 and IL-17 production (MPLA). Targeting equine DC with allergens fused to DC-binding peptides enhances antigen-uptake and T-cell activation and may be useful in increasing the equine immune response against recombinant antigens. Combination of DC-binding peptide protein fusions with adjuvants is necessary to appropriately skew the resulting immune response, depending on intended use. Combination with MPLA is a promising option for improvement of AIT efficacy in horses, while combination with CpG-ODN increases the effector immune response to recombinant antigens." @default.
• W3212784225 created "2021-11-22" @default.
• W3212784225 creator A5010547558 @default.
• W3212784225 creator A5020922147 @default.
• W3212784225 creator A5024572016 @default.
• W3212784225 creator A5035242879 @default.
• W3212784225 creator A5045644061 @default.
• W3212784225 creator A5075477708 @default.
• W3212784225 creator A5084699975 @default.
• W3212784225 creator A5088331902 @default.
• W3212784225 date "2022-01-01" @default.
• W3212784225 modified "2023-10-17" @default.
• W3212784225 title "An allergen-fused dendritic cell-binding peptide enhances in vitro proliferation of equine T-cells and cytokine production" @default.
• W3212784225 cites W1557759563 @default.
• W3212784225 cites W1576543020 @default.
• W3212784225 cites W1761774159 @default.
• W3212784225 cites W1963954205 @default.
• W3212784225 cites W1965810984 @default.
• W3212784225 cites W1969251366 @default.
• W3212784225 cites W1982074770 @default.
• W3212784225 cites W1985719575 @default.
• W3212784225 cites W1989936232 @default.
• W3212784225 cites W2003623305 @default.
• W3212784225 cites W2009621891 @default.
• W3212784225 cites W2010374650 @default.
• W3212784225 cites W2011261717 @default.
• W3212784225 cites W2017381385 @default.
• W3212784225 cites W2034963119 @default.
• W3212784225 cites W2058688703 @default.
• W3212784225 cites W2064696967 @default.
• W3212784225 cites W2073040443 @default.
• W3212784225 cites W2079263073 @default.
• W3212784225 cites W2089367686 @default.
• W3212784225 cites W2092005274 @default.
• W3212784225 cites W2106032198 @default.
• W3212784225 cites W2109834092 @default.
• W3212784225 cites W2116763454 @default.
• W3212784225 cites W2117895093 @default.
• W3212784225 cites W2120588519 @default.
• W3212784225 cites W2132138828 @default.
• W3212784225 cites W2136837710 @default.
• W3212784225 cites W2143847836 @default.
• W3212784225 cites W2145921416 @default.
• W3212784225 cites W2146591325 @default.
• W3212784225 cites W2158611413 @default.
• W3212784225 cites W2167043340 @default.
• W3212784225 cites W2169805815 @default.
• W3212784225 cites W2215410155 @default.
• W3212784225 cites W2281931894 @default.
• W3212784225 cites W2289297625 @default.
• W3212784225 cites W2320610943 @default.
• W3212784225 cites W2548351853 @default.
• W3212784225 cites W2553071892 @default.
• W3212784225 cites W2553761409 @default.
• W3212784225 cites W2626840463 @default.
• W3212784225 cites W2756993541 @default.
• W3212784225 cites W2763054302 @default.
• W3212784225 cites W2795753611 @default.
• W3212784225 cites W2799487194 @default.
• W3212784225 cites W2947736208 @default.
• W3212784225 cites W2949562804 @default.
• W3212784225 cites W2990976559 @default.
• W3212784225 cites W3048863920 @default.
• W3212784225 cites W3093766447 @default.
• W3212784225 doi "https://doi.org/10.1016/j.vetimm.2021.110351" @default.
• W3212784225 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34800874" @default.
• W3212784225 hasPublicationYear "2022" @default.
• W3212784225 type Work @default.
• W3212784225 sameAs 3212784225 @default.
• W3212784225 citedByCount "2" @default.
• W3212784225 countsByYear W32127842252022 @default.
• W3212784225 countsByYear W32127842252023 @default.
• W3212784225 crossrefType "journal-article" @default.
• W3212784225 hasAuthorship W3212784225A5010547558 @default.
• W3212784225 hasAuthorship W3212784225A5020922147 @default.
• W3212784225 hasAuthorship W3212784225A5024572016 @default.
• W3212784225 hasAuthorship W3212784225A5035242879 @default.
• W3212784225 hasAuthorship W3212784225A5045644061 @default.
• W3212784225 hasAuthorship W3212784225A5075477708 @default.
• W3212784225 hasAuthorship W3212784225A5084699975 @default.
• W3212784225 hasAuthorship W3212784225A5088331902 @default.
• W3212784225 hasBestOaLocation W32127842251 @default.
• W3212784225 hasConcept C137061746 @default.
• W3212784225 hasConcept C147483822 @default.
• W3212784225 hasConcept C202751555 @default.
• W3212784225 hasConcept C203014093 @default.
• W3212784225 hasConcept C2776090121 @default.
• W3212784225 hasConcept C2777701055 @default.
• W3212784225 hasConcept C2778170410 @default.
• W3212784225 hasConcept C2780868878 @default.
• W3212784225 hasConcept C55493867 @default.
• W3212784225 hasConcept C67662055 @default.
• W3212784225 hasConcept C86803240 @default.
• W3212784225 hasConcept C8891405 @default.
• W3212784225 hasConceptScore W3212784225C137061746 @default.
• W3212784225 hasConceptScore W3212784225C147483822 @default.
• W3212784225 hasConceptScore W3212784225C202751555 @default.
• W3212784225 hasConceptScore W3212784225C203014093 @default.

• next