Matches in SemOpenAlex for { <https://semopenalex.org/work/W3212784890> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W3212784890 endingPage "56" @default.
- W3212784890 startingPage "50" @default.
- W3212784890 abstract "SUMMARY Although the green seaweed Ulva is one of the most common seaweeds in the coastal regions with well‐studied ecological characteristics, few reverse genetic technologies have been developed for it. The clustered regularly interspaced short palindromic repeats (CRISPR)‐Cas9 system is a simple genome‐editing technology based on a ribonucleoprotein (RNP) complex composed of an endonuclease and programmable RNA to target particular DNA sequences. Genome editing makes it possible to generate mutations on a target gene in non‐model organisms without established transgenic technologies. In this study, we applied the CRISPR‐Cas9 RNP genome‐editing system to the green seaweed Ulva prolifera , using polyethylene glycol (PEG)‐mediated transfection. Our experimental system disrupts a single gene ( UpAPT ) encoding adenine phosphoribosyl transferase (APT) and generates a resistant phenotype for gametophytes cultured in a medium with toxic compound 2‐fluoroadenine. The PEG‐mediated transfection used for gametes resulted in 2‐fluoroadenine‐resistant strains containing short indels or substitutions on UpAPT . Our results showed that the CRISPR‐Cas9 system with PEG‐mediated transfection was efficient for genome editing in Ulva ." @default.
- W3212784890 created "2021-11-22" @default.
- W3212784890 creator A5045313013 @default.
- W3212784890 creator A5077350209 @default.
- W3212784890 creator A5078351196 @default.
- W3212784890 date "2021-11-03" @default.
- W3212784890 modified "2023-10-15" @default.
- W3212784890 title "Genome editing using a <scp>DNA</scp> ‐free clustered regularly interspaced short palindromic repeats <scp>‐Cas9</scp> system in green seaweed <scp> <i>Ulva prolifera</i> </scp>" @default.
- W3212784890 cites W1787401975 @default.
- W3212784890 cites W1822640581 @default.
- W3212784890 cites W1983278548 @default.
- W3212784890 cites W1993484667 @default.
- W3212784890 cites W1994371695 @default.
- W3212784890 cites W1997821934 @default.
- W3212784890 cites W2011095723 @default.
- W3212784890 cites W2045435533 @default.
- W3212784890 cites W2078452946 @default.
- W3212784890 cites W2094285812 @default.
- W3212784890 cites W2096261947 @default.
- W3212784890 cites W2098133034 @default.
- W3212784890 cites W2120503144 @default.
- W3212784890 cites W2145782793 @default.
- W3212784890 cites W2164695230 @default.
- W3212784890 cites W2169259933 @default.
- W3212784890 cites W2311203695 @default.
- W3212784890 cites W2340429497 @default.
- W3212784890 cites W2395418519 @default.
- W3212784890 cites W2443008206 @default.
- W3212784890 cites W2470852603 @default.
- W3212784890 cites W2479120789 @default.
- W3212784890 cites W2502802553 @default.
- W3212784890 cites W2551575280 @default.
- W3212784890 cites W2559191318 @default.
- W3212784890 cites W2572900050 @default.
- W3212784890 cites W2756111581 @default.
- W3212784890 cites W2771068972 @default.
- W3212784890 cites W2781851416 @default.
- W3212784890 cites W2890283697 @default.
- W3212784890 cites W2892105462 @default.
- W3212784890 cites W2922354312 @default.
- W3212784890 cites W2923103077 @default.
- W3212784890 cites W2946957748 @default.
- W3212784890 cites W3003294779 @default.
- W3212784890 cites W3004286261 @default.
- W3212784890 cites W3158569434 @default.
- W3212784890 cites W3165356543 @default.
- W3212784890 cites W927857605 @default.
- W3212784890 doi "https://doi.org/10.1111/pre.12472" @default.
- W3212784890 hasPublicationYear "2021" @default.
- W3212784890 type Work @default.
- W3212784890 sameAs 3212784890 @default.
- W3212784890 citedByCount "6" @default.
- W3212784890 countsByYear W32127848902023 @default.
- W3212784890 crossrefType "journal-article" @default.
- W3212784890 hasAuthorship W3212784890A5045313013 @default.
- W3212784890 hasAuthorship W3212784890A5077350209 @default.
- W3212784890 hasAuthorship W3212784890A5078351196 @default.
- W3212784890 hasConcept C104317684 @default.
- W3212784890 hasConcept C132455925 @default.
- W3212784890 hasConcept C141231307 @default.
- W3212784890 hasConcept C144501496 @default.
- W3212784890 hasConcept C54355233 @default.
- W3212784890 hasConcept C70721500 @default.
- W3212784890 hasConcept C86803240 @default.
- W3212784890 hasConcept C98108389 @default.
- W3212784890 hasConceptScore W3212784890C104317684 @default.
- W3212784890 hasConceptScore W3212784890C132455925 @default.
- W3212784890 hasConceptScore W3212784890C141231307 @default.
- W3212784890 hasConceptScore W3212784890C144501496 @default.
- W3212784890 hasConceptScore W3212784890C54355233 @default.
- W3212784890 hasConceptScore W3212784890C70721500 @default.
- W3212784890 hasConceptScore W3212784890C86803240 @default.
- W3212784890 hasConceptScore W3212784890C98108389 @default.
- W3212784890 hasFunder F4320334764 @default.
- W3212784890 hasIssue "1" @default.
- W3212784890 hasLocation W32127848901 @default.
- W3212784890 hasOpenAccess W3212784890 @default.
- W3212784890 hasPrimaryLocation W32127848901 @default.
- W3212784890 hasRelatedWork W2134268326 @default.
- W3212784890 hasRelatedWork W2337751047 @default.
- W3212784890 hasRelatedWork W2437118042 @default.
- W3212784890 hasRelatedWork W2603161682 @default.
- W3212784890 hasRelatedWork W2667943050 @default.
- W3212784890 hasRelatedWork W2807464733 @default.
- W3212784890 hasRelatedWork W2903635918 @default.
- W3212784890 hasRelatedWork W2960163646 @default.
- W3212784890 hasRelatedWork W3000623258 @default.
- W3212784890 hasRelatedWork W3120857162 @default.
- W3212784890 hasVolume "70" @default.
- W3212784890 isParatext "false" @default.
- W3212784890 isRetracted "false" @default.
- W3212784890 magId "3212784890" @default.
- W3212784890 workType "article" @default.