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- W3213014671 abstract "Early and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses at the point-of-care is crucial for reducing disease transmission during the current pandemic and future flu seasons. To prepare for potential cocirculation of these two viruses, we report a valve-enabled, paper-based sample preparation device integrated with isothermal amplification for their simultaneous detection. The device incorporates (1) virus lysis and RNA enrichment, enabled by ball-based valves for sequential delivery of reagents with no pipet requirement, (2) reverse transcription loop-mediated isothermal amplification, carried out in a coffee mug, and (3) colorimetric detection. We have used the device for simultaneously detecting inactivated SARS-CoV-2 and influenza A H1N1 viruses in 50 min, with limits of detection at 2 and 6 genome equivalents, respectively. The device was further demonstrated to detect both viruses in environmental samples." @default.
- W3213014671 created "2021-11-22" @default.
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- W3213014671 date "2021-11-12" @default.
- W3213014671 modified "2023-10-16" @default.
- W3213014671 title "A Valve-Enabled Sample Preparation Device with Isothermal Amplification for Multiplexed Virus Detection at the Point-of-Care" @default.
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- W3213014671 doi "https://doi.org/10.1021/acssensors.1c01718" @default.
- W3213014671 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8609915" @default.
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