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- W3213064327 abstract "Gelatin methacrylate (GelMA) hydrogels are a promising material for use in a variety of tissue engineering applications. Herein, we focused on identifying the optimal irradiation time necessary to photopolymerize GelMA hydrogels with visible blue light in a manner that did not adversely impact the biophysical properties of these cell-containing gels. We assessed the toxic effects of different irradiation times (3, 5, 10, 20 and 40 seconds) on BMMSCs encapsulated in a GelMA hydrogel using lithium phenyl-2,4,6 trimethylbenzoylphosphinate (LAP) as a photoinitiator. Both CCK-8 assays and Live-Dead staining were used to measure BMMSCs viability. We observed increasing compression strength as a function of increased irradiation time, although this corresponded to a reduction in swelling ratio and pore sizes. We ultimately found that when using LAP as a photoinitiator, the optimal irradiation time was 5–10 seconds, which was suitable for bone tissue engineering application. Ultimately we determined that a 5 second irradiation time was optimal for studies of encapsulated stem cells." @default.
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- W3213064327 date "2022-01-01" @default.
- W3213064327 modified "2023-09-24" @default.
- W3213064327 title "The Effects of Irradiation Time on Gelatin Methacrylate Hydrogels Used for Bone Tissue Engineering" @default.
- W3213064327 doi "https://doi.org/10.1166/jbt.2022.2887" @default.
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