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• W3213092021 abstract "Abstract Harnessing the unique biochemical capabilities of non-model microorganisms would expand the array of biomanufacturing substrates, process conditions, and products. There are non-model microorganisms that fix nitrogen and carbon dioxide, derive energy from light, catabolize methane and lignin-derived aromatics, are tolerant to physiochemical stresses and harsh environmental conditions, store lipids in large quantities, and produce hydrogen. Model microorganisms often only break down simple sugars and require low stress conditions, but they have been engineered for the sustainable manufacture of numerous products, such as fragrances, pharmaceuticals, cosmetics, surfactants, and specialty chemicals, often by using tools from synthetic biology. Transferring complex pathways with all of the needed cofactors, energy sources, and cellular conditions from a non-model microorganism to a common chassis has proven to be exceedingly difficult. Utilization of unique biochemical capabilities could also be achieved by engineering the host; although, synthetic biology tools developed for model microbes often do not perform as designed in other microorganisms. The metabolically versatile Rhodopseudomonas palustris CGA009, a purple non-sulfur bacterium, catabolizes aromatic compounds derived from lignin in both aerobic and anaerobic conditions and can use light, inorganic, and organic compounds for its source of energy. R. palustris utilizes three nitrogenase isozymes to fulfill its nitrogen requirements while also generating hydrogen. Furthermore, the bacterium produces two forms of RuBisCo in response to carbon dioxide/bicarbonate availability. While this potential chassis harbors many beneficial traits, stable heterologous gene expression has been problematic due to its intrinsic resistance to many antibiotics and the lack of synthetic biology parts investigated in this microbe. To address these problems, we have characterized gene expression and plasmid maintenance for different selection markers, started a synthetic biology toolbox specifically for the photosynthetic R. palustris , including origins of replication, fluorescent reporters, terminators, and 5’ untranslated regions, and employed the microbe’s endogenous plasmid for exogenous protein production. This work provides essential synthetic biology tools for engineering R. palustris ’ many unique biochemical processes and has helped define the principles for expressing heterologous genes in this promising microbe through a methodology that could be applied to other non-model microorganisms." @default.
• W3213092021 created "2021-11-22" @default.
• W3213092021 creator A5027056402 @default.
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• W3213092021 date "2021-11-04" @default.
• W3213092021 modified "2023-09-23" @default.
• W3213092021 title "Synthetic Biology Tool Development Advances Predictable Gene Expression in the Metabolically Versatile Soil Bacterium Rhodopseudomonas palustris" @default.
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• W3213092021 cites W1966549106 @default.
• W3213092021 cites W1967382476 @default.
• W3213092021 cites W1968196536 @default.
• W3213092021 cites W1968779075 @default.
• W3213092021 cites W1972549326 @default.
• W3213092021 cites W1979197349 @default.
• W3213092021 cites W1990923586 @default.
• W3213092021 cites W1991383594 @default.
• W3213092021 cites W2013852543 @default.
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• W3213092021 cites W2021933218 @default.
• W3213092021 cites W2029022533 @default.
• W3213092021 cites W2029512487 @default.
• W3213092021 cites W2030618280 @default.
• W3213092021 cites W2035761995 @default.
• W3213092021 cites W2036874513 @default.
• W3213092021 cites W2036917159 @default.
• W3213092021 cites W2041540935 @default.
• W3213092021 cites W2044910245 @default.
• W3213092021 cites W2047360542 @default.
• W3213092021 cites W2055194955 @default.
• W3213092021 cites W2063028509 @default.
• W3213092021 cites W2067988403 @default.
• W3213092021 cites W2071938573 @default.
• W3213092021 cites W2072324819 @default.
• W3213092021 cites W2073618656 @default.
• W3213092021 cites W2076336907 @default.
• W3213092021 cites W2093560200 @default.
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• W3213092021 cites W2128556638 @default.
• W3213092021 cites W2128834757 @default.
• W3213092021 cites W2129741035 @default.
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• W3213092021 cites W2142444085 @default.
• W3213092021 cites W2143467231 @default.
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• W3213092021 cites W2152165550 @default.
• W3213092021 cites W2166744717 @default.
• W3213092021 cites W2168059482 @default.
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• W3213092021 cites W2267069928 @default.
• W3213092021 cites W2276788064 @default.
• W3213092021 cites W2324591890 @default.
• W3213092021 cites W2337171493 @default.
• W3213092021 cites W2400520117 @default.
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• W3213092021 cites W2524607249 @default.
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• W3213092021 cites W2607408717 @default.
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• W3213092021 cites W2737972386 @default.
• W3213092021 cites W2738666591 @default.
• W3213092021 cites W2766189659 @default.
• W3213092021 cites W2775803673 @default.
• W3213092021 cites W2784680706 @default.
• W3213092021 cites W2806616369 @default.
• W3213092021 cites W2891382517 @default.
• W3213092021 cites W2892624118 @default.
• W3213092021 cites W2901363692 @default.
• W3213092021 cites W2902853677 @default.
• W3213092021 cites W2929467904 @default.
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• W3213092021 cites W2954592801 @default.
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• W3213092021 doi "https://doi.org/10.1101/2021.11.01.466785" @default.
• W3213092021 hasPublicationYear "2021" @default.
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