FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3213301459> ?p ?o ?g. }

• W3213301459 endingPage "12957" @default.
• W3213301459 startingPage "12947" @default.
• W3213301459 abstract "Knowledge about the structural and dynamic properties of proteins that form membrane-less organelles in cells via liquid-liquid phase separation (LLPS) is required for understanding the process at a molecular level. We used spin labeling and electron paramagnetic resonance (EPR) spectroscopy to investigate the dynamic properties (rotational diffusion) of the low complexity N-terminal domain of cytoplasmic polyadenylation element binding-4 protein (CPEB4NTD) across its LLPS transition, which takes place with increasing temperature. We report the coexistence of three spin labeled CPEB4NTD (CPEB4*) populations with distinct dynamic properties representing different conformational spaces, both before and within the LLPS state. Monomeric CPEB4* exhibiting fast motion defines population I and shows low abundance prior to and following LLPS. Populations II and III are part of CPEB4* assemblies where II corresponds to loose conformations with intermediate range motions and population III represents compact conformations with strongly attenuated motions. As the temperature increased the population of component II increased reversibly at the expense of component III, indicating the existence of an III ⇌ II equilibrium. We correlated the macroscopic LLPS properties with the III ⇌ II exchange process upon varying temperature and CPEB4* and salt concentrations. We hypothesized that weak transient intermolecular interactions facilitated by component II lead to LLPS, with the small assemblies integrated within the droplets. The LLPS transition, however, was not associated with a clear discontinuity in the correlation times and populations of the three components. Importantly, CPEB4NTD exhibits LLPS properties where droplet formation occurs from a preformed microscopic assembly rather than the monomeric protein molecules." @default.
• W3213301459 created "2021-11-22" @default.
• W3213301459 creator A5015468665 @default.
• W3213301459 creator A5022917512 @default.
• W3213301459 creator A5024576724 @default.
• W3213301459 creator A5031240068 @default.
• W3213301459 creator A5050428280 @default.
• W3213301459 creator A5063629719 @default.
• W3213301459 creator A5076914026 @default.
• W3213301459 creator A5080781064 @default.
• W3213301459 date "2021-11-17" @default.
• W3213301459 modified "2023-09-27" @default.
• W3213301459 title "Evolution of CPEB4 Dynamics Across its Liquid–Liquid Phase Separation Transition" @default.
• W3213301459 cites W1800445960 @default.
• W3213301459 cites W1912455695 @default.
• W3213301459 cites W1936657395 @default.
• W3213301459 cites W1952608303 @default.
• W3213301459 cites W1972146777 @default.
• W3213301459 cites W1978362603 @default.
• W3213301459 cites W1980084095 @default.
• W3213301459 cites W1986035298 @default.
• W3213301459 cites W1994519311 @default.
• W3213301459 cites W1995379884 @default.
• W3213301459 cites W2023551920 @default.
• W3213301459 cites W2026226960 @default.
• W3213301459 cites W2028718267 @default.
• W3213301459 cites W2038153950 @default.
• W3213301459 cites W2038272359 @default.
• W3213301459 cites W2039914635 @default.
• W3213301459 cites W2041488593 @default.
• W3213301459 cites W2046762491 @default.
• W3213301459 cites W2054352933 @default.
• W3213301459 cites W2070073978 @default.
• W3213301459 cites W2083939019 @default.
• W3213301459 cites W2089922879 @default.
• W3213301459 cites W2091646550 @default.
• W3213301459 cites W2096680110 @default.
• W3213301459 cites W2103813490 @default.
• W3213301459 cites W2128677332 @default.
• W3213301459 cites W2139066576 @default.
• W3213301459 cites W2146051607 @default.
• W3213301459 cites W2147440358 @default.
• W3213301459 cites W2150860983 @default.
• W3213301459 cites W2155413584 @default.
• W3213301459 cites W2155533444 @default.
• W3213301459 cites W2165581033 @default.
• W3213301459 cites W2467565876 @default.
• W3213301459 cites W2473312868 @default.
• W3213301459 cites W2547775432 @default.
• W3213301459 cites W2574905597 @default.
• W3213301459 cites W2597238462 @default.
• W3213301459 cites W2615201923 @default.
• W3213301459 cites W2722642526 @default.
• W3213301459 cites W2742396220 @default.
• W3213301459 cites W2743702295 @default.
• W3213301459 cites W2753162941 @default.
• W3213301459 cites W2755105920 @default.
• W3213301459 cites W2755486970 @default.
• W3213301459 cites W2756821926 @default.
• W3213301459 cites W2786469974 @default.
• W3213301459 cites W2792129356 @default.
• W3213301459 cites W2888375901 @default.
• W3213301459 cites W2905453715 @default.
• W3213301459 cites W2914907359 @default.
• W3213301459 cites W2916646997 @default.
• W3213301459 cites W2953265742 @default.
• W3213301459 cites W2969279439 @default.
• W3213301459 cites W2969743565 @default.
• W3213301459 cites W2973734810 @default.
• W3213301459 cites W2979601486 @default.
• W3213301459 cites W2980586256 @default.
• W3213301459 cites W3000212072 @default.
• W3213301459 cites W3005609806 @default.
• W3213301459 cites W3048742673 @default.
• W3213301459 cites W3096333719 @default.
• W3213301459 cites W3112228621 @default.
• W3213301459 cites W3112376646 @default.
• W3213301459 cites W3119082520 @default.
• W3213301459 cites W3124178049 @default.
• W3213301459 cites W3131479422 @default.
• W3213301459 cites W3133546102 @default.
• W3213301459 cites W3156525837 @default.
• W3213301459 doi "https://doi.org/10.1021/acs.jpcb.1c06696" @default.
• W3213301459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34787433" @default.
• W3213301459 hasPublicationYear "2021" @default.
• W3213301459 type Work @default.
• W3213301459 sameAs 3213301459 @default.
• W3213301459 citedByCount "5" @default.
• W3213301459 countsByYear W32133014592022 @default.
• W3213301459 countsByYear W32133014592023 @default.
• W3213301459 crossrefType "journal-article" @default.
• W3213301459 hasAuthorship W3213301459A5015468665 @default.
• W3213301459 hasAuthorship W3213301459A5022917512 @default.
• W3213301459 hasAuthorship W3213301459A5024576724 @default.
• W3213301459 hasAuthorship W3213301459A5031240068 @default.
• W3213301459 hasAuthorship W3213301459A5050428280 @default.
• W3213301459 hasAuthorship W3213301459A5063629719 @default.
• W3213301459 hasAuthorship W3213301459A5076914026 @default.

• next