FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3213302600> ?p ?o ?g. }

• W3213302600 abstract "The Mediator complex is an evolutionarily conserved multi-subunit protein complex that plays major roles in transcriptional activation and is essential for cell growth, proliferation, and differentiation. Recent studies revealed that some Mediator subunits formed nuclear condensates that may facilitate enhancer-promoter interactions and gene activation. The assembly, regulation, and functions of these nuclear condensates remain to be further understood.We found that Med15, a subunit in the tail module of the Mediator complex, formed nuclear condensates through a novel mechanism. Nuclear foci of Med15 were detected by both immunostaining of endogenous proteins and live cell imaging. Like Med1 foci and many other biomolecular condensates, Med15 foci were sensitive to 1, 6-Hexanediol and showed rapid recovery during fluorescence recovery after photobleaching. Interestingly, overexpressing DYRK3, a dual-specificity kinase that controls the phase transition of membraneless organelles, appeared to disrupt Med1 foci and Med15 foci. We identified two regions that are required to form Med15 nuclear condensates: the glutamine-rich intrinsically disordered region (IDR) and a short downstream hydrophobic motif. The optodroplet assay revealed that both the IDR and the C-terminal region of Med15 contributed to intracellular phase separation.We identified that the Mediator complex subunit Med15 formed nuclear condensates and characterized their features in living cells. Our work suggests that Med15 plays a role in the assembly of transcription coactivator condensates in the nucleus and identifies Med15 regions that contribute to phase separation." @default.
• W3213302600 created "2021-11-22" @default.
• W3213302600 creator A5008056710 @default.
• W3213302600 creator A5014736137 @default.
• W3213302600 creator A5037384654 @default.
• W3213302600 creator A5042006304 @default.
• W3213302600 creator A5067988089 @default.
• W3213302600 creator A5068551922 @default.
• W3213302600 creator A5080964131 @default.
• W3213302600 date "2021-11-17" @default.
• W3213302600 modified "2023-10-18" @default.
• W3213302600 title "Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells" @default.
• W3213302600 cites W1549536215 @default.
• W3213302600 cites W1910902099 @default.
• W3213302600 cites W1983825024 @default.
• W3213302600 cites W2024368545 @default.
• W3213302600 cites W2076110140 @default.
• W3213302600 cites W2085978470 @default.
• W3213302600 cites W2104746705 @default.
• W3213302600 cites W2108080167 @default.
• W3213302600 cites W2120156669 @default.
• W3213302600 cites W2139066576 @default.
• W3213302600 cites W2257762260 @default.
• W3213302600 cites W2349918789 @default.
• W3213302600 cites W2566521622 @default.
• W3213302600 cites W2590568317 @default.
• W3213302600 cites W2603000633 @default.
• W3213302600 cites W2754310332 @default.
• W3213302600 cites W2755486970 @default.
• W3213302600 cites W2756821926 @default.
• W3213302600 cites W2772832167 @default.
• W3213302600 cites W2791474337 @default.
• W3213302600 cites W2797600975 @default.
• W3213302600 cites W2804270751 @default.
• W3213302600 cites W2808847099 @default.
• W3213302600 cites W2809057270 @default.
• W3213302600 cites W2809265915 @default.
• W3213302600 cites W2810246680 @default.
• W3213302600 cites W2883067277 @default.
• W3213302600 cites W2884082692 @default.
• W3213302600 cites W2899914600 @default.
• W3213302600 cites W2901209536 @default.
• W3213302600 cites W2914907359 @default.
• W3213302600 cites W2941579711 @default.
• W3213302600 cites W2942748350 @default.
• W3213302600 cites W2966567728 @default.
• W3213302600 cites W3119441281 @default.
• W3213302600 cites W3153771064 @default.
• W3213302600 doi "https://doi.org/10.1186/s12915-021-01178-y" @default.
• W3213302600 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8597291" @default.
• W3213302600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34789250" @default.
• W3213302600 hasPublicationYear "2021" @default.
• W3213302600 type Work @default.
• W3213302600 sameAs 3213302600 @default.
• W3213302600 citedByCount "9" @default.
• W3213302600 countsByYear W32133026002022 @default.
• W3213302600 countsByYear W32133026002023 @default.
• W3213302600 crossrefType "journal-article" @default.
• W3213302600 hasAuthorship W3213302600A5008056710 @default.
• W3213302600 hasAuthorship W3213302600A5014736137 @default.
• W3213302600 hasAuthorship W3213302600A5037384654 @default.
• W3213302600 hasAuthorship W3213302600A5042006304 @default.
• W3213302600 hasAuthorship W3213302600A5067988089 @default.
• W3213302600 hasAuthorship W3213302600A5068551922 @default.
• W3213302600 hasAuthorship W3213302600A5080964131 @default.
• W3213302600 hasBestOaLocation W32133026001 @default.
• W3213302600 hasConcept C104292427 @default.
• W3213302600 hasConcept C104317684 @default.
• W3213302600 hasConcept C111936080 @default.
• W3213302600 hasConcept C190062978 @default.
• W3213302600 hasConcept C2107291 @default.
• W3213302600 hasConcept C2780114586 @default.
• W3213302600 hasConcept C29512474 @default.
• W3213302600 hasConcept C54355233 @default.
• W3213302600 hasConcept C59157529 @default.
• W3213302600 hasConcept C86339819 @default.
• W3213302600 hasConcept C86803240 @default.
• W3213302600 hasConcept C95444343 @default.
• W3213302600 hasConceptScore W3213302600C104292427 @default.
• W3213302600 hasConceptScore W3213302600C104317684 @default.
• W3213302600 hasConceptScore W3213302600C111936080 @default.
• W3213302600 hasConceptScore W3213302600C190062978 @default.
• W3213302600 hasConceptScore W3213302600C2107291 @default.
• W3213302600 hasConceptScore W3213302600C2780114586 @default.
• W3213302600 hasConceptScore W3213302600C29512474 @default.
• W3213302600 hasConceptScore W3213302600C54355233 @default.
• W3213302600 hasConceptScore W3213302600C59157529 @default.
• W3213302600 hasConceptScore W3213302600C86339819 @default.
• W3213302600 hasConceptScore W3213302600C86803240 @default.
• W3213302600 hasConceptScore W3213302600C95444343 @default.
• W3213302600 hasFunder F4320321001 @default.
• W3213302600 hasFunder F4320321160 @default.
• W3213302600 hasFunder F4320326705 @default.
• W3213302600 hasIssue "1" @default.
• W3213302600 hasLocation W32133026001 @default.
• W3213302600 hasLocation W32133026002 @default.
• W3213302600 hasLocation W32133026003 @default.
• W3213302600 hasLocation W32133026004 @default.
• W3213302600 hasOpenAccess W3213302600 @default.
• W3213302600 hasPrimaryLocation W32133026001 @default.

• next