FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3213532008> ?p ?o ?g. }

• W3213532008 endingPage "108845" @default.
• W3213532008 startingPage "108845" @default.
• W3213532008 abstract "Diabetic retinopathy (DR) is the leading cause of vision impairment in working age adults. In addition to hyperglycemia, retinal inflammation is an important driving factor for DR development. Although DR is clinically described as diabetes-induced damage to the retinal blood vessels, several studies have reported that metabolic dysregulation occurs in the retina prior to the development of microvascular damage. The two most commonly affected metabolic pathways in diabetic conditions are glycolysis and the glutamate pathway. We investigated the role of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutamine synthetase (GS) in an in-vitro model of DR incorporating high glucose and pro-inflammatory cytokines. We found that GAPDH and GS enzyme activity were not significantly affected in hyperglycemic conditions or after exposure to cytokines alone, but were significantly decreased in the DR model. This confirmed that pro-inflammatory cytokines IL-1β and TNFα enhance the hyperglycemic metabolic deficit. We further investigated metabolite and amino acid levels after specific pharmacological inhibition of GAPDH or GS in the absence/presence of pro-inflammatory cytokines. The results indicate that GAPDH inhibition increased glucose and addition of cytokines increased lactate and ATP levels and reduced glutamate levels. GS inhibition did not alter retinal metabolite levels but the addition of cytokines increased ATP levels and caused glutamate accumulation in Müller cells. We conclude that it is the action of pro-inflammatory cytokines concomitantly with the inhibition of the glycolytic or GS mediated glutamate recycling that contribute to metabolic dysregulation in DR. Therefore, in the absence of good glycemic control, therapeutic interventions aimed at regulating inflammation may prevent the onset of early metabolic imbalance in DR." @default.
• W3213532008 created "2021-11-22" @default.
• W3213532008 creator A5018971066 @default.
• W3213532008 creator A5050135304 @default.
• W3213532008 creator A5078076335 @default.
• W3213532008 date "2021-12-01" @default.
• W3213532008 modified "2023-10-16" @default.
• W3213532008 title "Glyceraldehyde-3-phosphate dehydrogenase and glutamine synthetase inhibition in the presence of pro-inflammatory cytokines contribute to the metabolic imbalance of diabetic retinopathy" @default.
• W3213532008 cites W1492161141 @default.
• W3213532008 cites W1557188560 @default.
• W3213532008 cites W1588159686 @default.
• W3213532008 cites W1661422265 @default.
• W3213532008 cites W1704545681 @default.
• W3213532008 cites W1964445339 @default.
• W3213532008 cites W1965037137 @default.
• W3213532008 cites W1982841054 @default.
• W3213532008 cites W1996478420 @default.
• W3213532008 cites W1996892917 @default.
• W3213532008 cites W1999371496 @default.
• W3213532008 cites W2003029394 @default.
• W3213532008 cites W2012833516 @default.
• W3213532008 cites W2013285816 @default.
• W3213532008 cites W2015072153 @default.
• W3213532008 cites W2022164177 @default.
• W3213532008 cites W2024081819 @default.
• W3213532008 cites W2030071933 @default.
• W3213532008 cites W2031740343 @default.
• W3213532008 cites W2033816859 @default.
• W3213532008 cites W2034548183 @default.
• W3213532008 cites W2035187305 @default.
• W3213532008 cites W2039057389 @default.
• W3213532008 cites W2044437167 @default.
• W3213532008 cites W2060141430 @default.
• W3213532008 cites W2076947492 @default.
• W3213532008 cites W2087468878 @default.
• W3213532008 cites W2093153094 @default.
• W3213532008 cites W2093202085 @default.
• W3213532008 cites W2096871163 @default.
• W3213532008 cites W2120276758 @default.
• W3213532008 cites W2121966728 @default.
• W3213532008 cites W2123307497 @default.
• W3213532008 cites W2127363066 @default.
• W3213532008 cites W2132949972 @default.
• W3213532008 cites W2136126428 @default.
• W3213532008 cites W2154133864 @default.
• W3213532008 cites W2161166912 @default.
• W3213532008 cites W2169061609 @default.
• W3213532008 cites W2471605942 @default.
• W3213532008 cites W2602597203 @default.
• W3213532008 cites W2735409202 @default.
• W3213532008 cites W2736767101 @default.
• W3213532008 cites W2762717139 @default.
• W3213532008 cites W2809489644 @default.
• W3213532008 cites W2888132367 @default.
• W3213532008 cites W2907714119 @default.
• W3213532008 cites W2955166256 @default.
• W3213532008 cites W2984000721 @default.
• W3213532008 cites W2995257393 @default.
• W3213532008 cites W3011632447 @default.
• W3213532008 cites W3080945733 @default.
• W3213532008 cites W3112009919 @default.
• W3213532008 doi "https://doi.org/10.1016/j.exer.2021.108845" @default.
• W3213532008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34800480" @default.
• W3213532008 hasPublicationYear "2021" @default.
• W3213532008 type Work @default.
• W3213532008 sameAs 3213532008 @default.
• W3213532008 citedByCount "4" @default.
• W3213532008 countsByYear W32135320082022 @default.
• W3213532008 countsByYear W32135320082023 @default.
• W3213532008 crossrefType "journal-article" @default.
• W3213532008 hasAuthorship W3213532008A5018971066 @default.
• W3213532008 hasAuthorship W3213532008A5050135304 @default.
• W3213532008 hasAuthorship W3213532008A5078076335 @default.
• W3213532008 hasConcept C126322002 @default.
• W3213532008 hasConcept C134018914 @default.
• W3213532008 hasConcept C134792803 @default.
• W3213532008 hasConcept C164027704 @default.
• W3213532008 hasConcept C170493617 @default.
• W3213532008 hasConcept C181199279 @default.
• W3213532008 hasConcept C20251656 @default.
• W3213532008 hasConcept C2776317432 @default.
• W3213532008 hasConcept C2776914184 @default.
• W3213532008 hasConcept C2777202666 @default.
• W3213532008 hasConcept C2777477808 @default.
• W3213532008 hasConcept C2779289688 @default.
• W3213532008 hasConcept C2779349466 @default.
• W3213532008 hasConcept C2779829184 @default.
• W3213532008 hasConcept C2780827179 @default.
• W3213532008 hasConcept C515207424 @default.
• W3213532008 hasConcept C55493867 @default.
• W3213532008 hasConcept C555293320 @default.
• W3213532008 hasConcept C61174792 @default.
• W3213532008 hasConcept C62231903 @default.
• W3213532008 hasConcept C71924100 @default.
• W3213532008 hasConcept C86803240 @default.
• W3213532008 hasConceptScore W3213532008C126322002 @default.
• W3213532008 hasConceptScore W3213532008C134018914 @default.
• W3213532008 hasConceptScore W3213532008C134792803 @default.

• next