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• W3213843314 abstract "This protocol describes a step-by-step chemical synthesis approach to prepare N3 -methylcytidine (m3 C) and its phosphoramidite. The method for synthesizing m3 C starts from commercially available cytidine, and proceeds via N3 -methylation in the presence of MeI, which generates the N3 -methylcytidine (m3 C) nucleoside, followed by the installation of several protecting groups at sites that include the 5'-hydroxyl group (4,4'-dimethoxytrityl protection), the 4-amino group (benzoyl protection), and the 2'-hydroxyl group (tert-butyldimethylsilyl, TBDMS, protection). Standard phosphoramidite chemistry is applied to prepare the final m3 C phosphoramidite for solid-phase synthesis of a series of RNA oligonucleotides. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Synthesis of N3 -methylcytidine (m3 C) and its phosphoramidite Basic Protocol 2: Automated synthesis of m3 C modified RNA oligonucleotides." @default.
• W3213843314 created "2021-11-22" @default.
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• W3213843314 date "2021-11-01" @default.
• W3213843314 modified "2023-10-16" @default.
• W3213843314 title "Synthesis and Purification of N ³ ‐Methylcytidine (m ³ C) Modified RNA Oligonucleotides" @default.
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• W3213843314 doi "https://doi.org/10.1002/cpz1.307" @default.
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• W3213843314 hasPublicationYear "2021" @default.
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