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• W3213864090 abstract "Abstract Nab2 encodes the Drosophila melanogaster member of a conserved family of zinc finger polyadenosine RNA-binding proteins (RBPs) linked to multiple steps in post-transcriptional regulation. Mutation of the Nab2 human ortholog ZC3H14 gives rise to an autosomal recessive intellectual disability but understanding of Nab2/ZC3H14 function in metazoan nervous systems is limited, in part because no comprehensive identification of metazoan Nab2/ZC3H14-associated RNA transcripts has yet been conducted. Moreover, many Nab2/ZC3H14 functional protein partnerships remain unidentified. Here, we present evidence that Nab2 genetically interacts with Ataxin-2 (Atx2), which encodes a neuronal translational regulator, and that these factors coordinately regulate neuronal morphology, circadian behavior, and adult viability. We then present the first high-throughput identifications of Nab2- and Atx2-associated RNAs in Drosophila brain neurons using RNA immunoprecipitation-sequencing (RIP-Seq). Critically, the RNA interactomes of each RBP overlap, and Nab2 exhibits high specificity in its RNA associations in neurons in vivo, associating with a small fraction of all polyadenylated RNAs. The identities of shared associated transcripts (e.g., drk, me31B, stai) and of transcripts specific to Nab2 or Atx2 (e.g., Arpc2 and tea) promise insight into neuronal functions of, and genetic interactions between, each RBP. Consistent with prior biochemical studies, Nab2-associated neuronal RNAs are overrepresented for internal A-rich motifs, suggesting these sequences may partially mediate Nab2 target selection. These data support a model where Nab2 functionally opposes Atx2 in neurons, demonstrate Nab2 shares associated neuronal RNAs with Atx2, and reveal Drosophila Nab2 associates with a more specific subset of polyadenylated mRNAs than its polyadenosine affinity alone may suggest." @default.
• W3213864090 created "2021-11-22" @default.
• W3213864090 creator A5005185027 @default.
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• W3213864090 creator A5081798922 @default.
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• W3213864090 creator A5089802054 @default.
• W3213864090 date "2022-01-01" @default.
• W3213864090 modified "2023-10-15" @default.
• W3213864090 title "The disease-associated proteins <i>Drosophila</i> Nab2 and Ataxin-2 interact with shared RNAs and coregulate neuronal morphology" @default.
• W3213864090 cites W1539835583 @default.
• W3213864090 cites W1571325922 @default.
• W3213864090 cites W1609312951 @default.
• W3213864090 cites W1697175964 @default.
• W3213864090 cites W1828379386 @default.
• W3213864090 cites W1885358257 @default.
• W3213864090 cites W1886240449 @default.
• W3213864090 cites W1963541985 @default.
• W3213864090 cites W1968483102 @default.
• W3213864090 cites W1970396586 @default.
• W3213864090 cites W1971047357 @default.
• W3213864090 cites W1971442608 @default.
• W3213864090 cites W1972399183 @default.
• W3213864090 cites W1973984431 @default.
• W3213864090 cites W1976518882 @default.
• W3213864090 cites W1977300363 @default.
• W3213864090 cites W1979527364 @default.
• W3213864090 cites W1980333298 @default.
• W3213864090 cites W1981395373 @default.
• W3213864090 cites W1984486655 @default.
• W3213864090 cites W1986383547 @default.
• W3213864090 cites W1989619236 @default.
• W3213864090 cites W1997160430 @default.
• W3213864090 cites W2001932825 @default.
• W3213864090 cites W2003497513 @default.
• W3213864090 cites W2007410711 @default.
• W3213864090 cites W2010081999 @default.
• W3213864090 cites W2010231891 @default.
• W3213864090 cites W2010970917 @default.
• W3213864090 cites W2012757283 @default.
• W3213864090 cites W2016332933 @default.
• W3213864090 cites W2021341670 @default.
• W3213864090 cites W2023090650 @default.
• W3213864090 cites W2025305489 @default.
• W3213864090 cites W2028279692 @default.
• W3213864090 cites W2030916925 @default.
• W3213864090 cites W2030924245 @default.
• W3213864090 cites W2032185656 @default.
• W3213864090 cites W2032418607 @default.
• W3213864090 cites W2032933116 @default.
• W3213864090 cites W2036338927 @default.
• W3213864090 cites W2037175112 @default.
• W3213864090 cites W2038424676 @default.
• W3213864090 cites W2046659090 @default.
• W3213864090 cites W2048054368 @default.
• W3213864090 cites W2049862491 @default.
• W3213864090 cites W2049980602 @default.
• W3213864090 cites W2051626758 @default.
• W3213864090 cites W2053758959 @default.
• W3213864090 cites W2058565567 @default.
• W3213864090 cites W2058579886 @default.
• W3213864090 cites W2060238201 @default.
• W3213864090 cites W2064215369 @default.
• W3213864090 cites W2071344712 @default.
• W3213864090 cites W2072267291 @default.
• W3213864090 cites W2073036589 @default.
• W3213864090 cites W2086039300 @default.
• W3213864090 cites W2088525343 @default.
• W3213864090 cites W2095881318 @default.
• W3213864090 cites W2096821445 @default.
• W3213864090 cites W2100270425 @default.
• W3213864090 cites W2100837269 @default.
• W3213864090 cites W2102309307 @default.
• W3213864090 cites W2103017472 @default.
• W3213864090 cites W2104866072 @default.
• W3213864090 cites W2106588029 @default.
• W3213864090 cites W2107444110 @default.
• W3213864090 cites W2110227151 @default.
• W3213864090 cites W2112942849 @default.
• W3213864090 cites W2113451695 @default.
• W3213864090 cites W2113671240 @default.
• W3213864090 cites W2115514152 @default.
• W3213864090 cites W2116635614 @default.
• W3213864090 cites W2117083472 @default.
• W3213864090 cites W2121339588 @default.
• W3213864090 cites W2122657443 @default.
• W3213864090 cites W2125624608 @default.
• W3213864090 cites W2126114662 @default.
• W3213864090 cites W2126990943 @default.
• W3213864090 cites W2127453064 @default.
• W3213864090 cites W2128672347 @default.
• W3213864090 cites W2137499975 @default.
• W3213864090 cites W2138196920 @default.
• W3213864090 cites W2138207763 @default.
• W3213864090 cites W2139587743 @default.
• W3213864090 cites W2140112946 @default.
• W3213864090 cites W2140952049 @default.
• W3213864090 cites W2141353816 @default.

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