FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3213893763> ?p ?o ?g. }

• W3213893763 endingPage "A994" @default.
• W3213893763 startingPage "A993" @default.
• W3213893763 abstract "Background Breast cancer(BC) is the second most common cause after lung cancer of malignant pleural effusions(MPEs),in approximately one third of all MPEs.Although,MPEs are relativity easy to be collated are still not well characterized in their cellular compositions. This opens new avenues to characterize the cellular milieu comprising the MPE, as it has the potential to be highly informative about mutational markers and immune response –ultimately guiding targeted therapy and predicting therapeutic outcomes with their study. The proposed study will characterize immune landscape of the cellular composition of MPE from patients with metastatic breast carcinoma and characterize their relationship with clinicopathologic features in these patients. Abstract 945 Figure 1 Comparison between the cell block in H-E and mIF expression CK, CD68 and CD3 Abstract 945 Figure 2 Composite image in mIF expressing 8 markers. In higher magnification is possible to observe the co expression of CK+Ki67+, CK PDL1, CD3+Foxp3+ and CD3+CD8+ Abstract 945 Table 1 Results: cell phenotypes in percentage in the six cases analyzed Abstract 945 Table 2 Clinical data of the six patients. L: left . R: right , BR : Breast cáncer, CRC: Colorrectal cáncer, NE: No evaluable , IDC : Invasive ductal carcinoma , CT: chemotherapy and BT : biotherapy * Last appointment of the patient. Methods Five microns thickness paraffin cell pellet blocks from six cases randomly selected of breast carcinoma MPE were stained using a quantitative multiplex immunofluorescence(mIF) panel containing 8 markers against pancytokeratin(CK), PD-L1, PD-1, CD3, CD8, Foxp3, CD68, Ki67, and DAPI (figure 1). Representative regions of interest were scanned using a multispectral scanner (Vectra Polaris) in high magnification (20x) to capture different cell populations. Markers co-expression were processed and analyzed using a quantitative image analysis software (InForm). The final results were obtained as absolute number of cells from each phenotype and were characterized with clinicopathologic features. Results We analyzed and stained six breast cancer MPE cases with previously optimized and validated mIF panel for formalin fixed and paraffin embedded (FFPE) tumor tissues against CK, CD3, CD68, CD8, Foxp3, Ki67, PD1 and PD-L1 (figure 2). The median cellular density was 5870.53 cells. Median for each marker: CK+ was presented in 75.9% (between malignant cells and reactive mesothelial cells) in these cells the expression of Ki67 was 8% and PD-L1+ was present in 0.2%.CD3+ was 0.72% and being the cytotoxic T-cells CD3+CD8+ was 12.13% of these cells and it expression for CD3+PD1+ was in 1.14% without concomitant expression for PD-L1. The median of the macrophages CD68+ was 8.1% of the total cells (table 2). Conclusions mIF is a promising tool to study diverse corporal effusion from different origin. Although more studies are needed, this new perspective can help us to resolve some clues and possible prognosis in advanced stages of BC. Reference Nicholas D T, Matthew A. S. Diagnosis and Management of Pleural Metastases and Malignant Effusion in Breast Cancer.En: Kirby I B, Edward M C, V. Suzanne K, William J. G. The Breast (Fifth Edition): Elsevier; 2018. P 934." @default.
• W3213893763 created "2021-11-22" @default.
• W3213893763 creator A5017635314 @default.
• W3213893763 creator A5031167674 @default.
• W3213893763 creator A5035200640 @default.
• W3213893763 creator A5041388280 @default.
• W3213893763 creator A5043477787 @default.
• W3213893763 creator A5088436268 @default.
• W3213893763 date "2021-11-01" @default.
• W3213893763 modified "2023-09-25" @default.
• W3213893763 title "945 Characterization of the immune landscape of malignant pleural effusion composition from patients with metastatic breast carcinoma: a pilot study" @default.
• W3213893763 doi "https://doi.org/10.1136/jitc-2021-sitc2021.945" @default.
• W3213893763 hasPublicationYear "2021" @default.
• W3213893763 type Work @default.
• W3213893763 sameAs 3213893763 @default.
• W3213893763 citedByCount "0" @default.
• W3213893763 crossrefType "journal-article" @default.
• W3213893763 hasAuthorship W3213893763A5017635314 @default.
• W3213893763 hasAuthorship W3213893763A5031167674 @default.
• W3213893763 hasAuthorship W3213893763A5035200640 @default.
• W3213893763 hasAuthorship W3213893763A5041388280 @default.
• W3213893763 hasAuthorship W3213893763A5043477787 @default.
• W3213893763 hasAuthorship W3213893763A5088436268 @default.
• W3213893763 hasBestOaLocation W32138937631 @default.
• W3213893763 hasConcept C121608353 @default.
• W3213893763 hasConcept C126322002 @default.
• W3213893763 hasConcept C142724271 @default.
• W3213893763 hasConcept C143998085 @default.
• W3213893763 hasConcept C167672396 @default.
• W3213893763 hasConcept C203014093 @default.
• W3213893763 hasConcept C2777546739 @default.
• W3213893763 hasConcept C3018521938 @default.
• W3213893763 hasConcept C502942594 @default.
• W3213893763 hasConcept C530470458 @default.
• W3213893763 hasConcept C71924100 @default.
• W3213893763 hasConcept C8891405 @default.
• W3213893763 hasConceptScore W3213893763C121608353 @default.
• W3213893763 hasConceptScore W3213893763C126322002 @default.
• W3213893763 hasConceptScore W3213893763C142724271 @default.
• W3213893763 hasConceptScore W3213893763C143998085 @default.
• W3213893763 hasConceptScore W3213893763C167672396 @default.
• W3213893763 hasConceptScore W3213893763C203014093 @default.
• W3213893763 hasConceptScore W3213893763C2777546739 @default.
• W3213893763 hasConceptScore W3213893763C3018521938 @default.
• W3213893763 hasConceptScore W3213893763C502942594 @default.
• W3213893763 hasConceptScore W3213893763C530470458 @default.
• W3213893763 hasConceptScore W3213893763C71924100 @default.
• W3213893763 hasConceptScore W3213893763C8891405 @default.
• W3213893763 hasIssue "Suppl 3" @default.
• W3213893763 hasLocation W32138937631 @default.
• W3213893763 hasLocation W32138937632 @default.
• W3213893763 hasOpenAccess W3213893763 @default.
• W3213893763 hasPrimaryLocation W32138937631 @default.
• W3213893763 hasRelatedWork W1971093760 @default.
• W3213893763 hasRelatedWork W1987252334 @default.
• W3213893763 hasRelatedWork W2048880462 @default.
• W3213893763 hasRelatedWork W2085896209 @default.
• W3213893763 hasRelatedWork W2140498192 @default.
• W3213893763 hasRelatedWork W2224319365 @default.
• W3213893763 hasRelatedWork W2409600456 @default.
• W3213893763 hasRelatedWork W2466200003 @default.
• W3213893763 hasRelatedWork W377899687 @default.
• W3213893763 hasRelatedWork W4206561586 @default.
• W3213893763 hasVolume "9" @default.
• W3213893763 isParatext "false" @default.
• W3213893763 isRetracted "false" @default.
• W3213893763 magId "3213893763" @default.
• W3213893763 workType "article" @default.