Matches in SemOpenAlex for { <https://semopenalex.org/work/W3214083809> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W3214083809 abstract "Author(s): Young, Thomas Dylan | Advisor(s): Weiss, Paul S. | Abstract: This dissertation describes investigations of the interfaces between useful micro-organisms and solid surfaces, such as electrodes. Many types of microbes are ecologically important and can be technologically useful. Electrochemically active bacteria are particularly interesting, because of their ability to couple their metabolisms with inorganic devices. For electrochemically active bacteria to engage electrodes, they must localize around the electrodes and establish electrically conductive pathways. To understand the microbial interaction with conductive materials, we investigated both the attachment characteristics of bacteria to surfaces as well as the properties of cellular appendages, which can provide extracellular electronic conductivity. We probed the electrical and mechanical properties of bacterial appendages with atomic force microscopy methods. The adhesiveness of Geobacter sulfurreducens cellular appendages was determined and compared against electrical conductivity and nanoscale morphology. The electron mobility of Geobacter sulfurreducens pili was validated with electrostatic force microscopy as well. Design principles for correlating atomic force microscopy measurements and optical microscopy are discussed.The attachment of Shewanella oneidensis to surfaces was investigated by measuring the number of adherent cells on surfaces through a combination of direct fluorescence measurement, counting colonies formed from cells removed by sonication, and microscopy of the bacteria-attached surfaces. More S. oneidensis cells attach to hydrophobic surfaces and mannosylated surfaces compared to bare gold.Using mannose-decorated glycopolymers to functionalize gold surfaces, we stimulated S. oneidensis bacterial colonization, enriched the wild-type strain of the bacteria against ΔmshA-D knockout strain during co-deposition, and induced where bacteria attach on a molecular pattern. Vibrio cholerae was also directed to a molecular pattern. The three-dimensional multivalency of the glycopolymers enhanced the persistence of attached bacteria on the surface more than surfaces functionalized with a single glycoside per molecule. Removing the attachment enhancement required equilibration between methyl α-D-mannopyranoside competitor and the cell culture. This requirement suggests the retention of cells on glycopolymer surfaces is kinetically controlled, and not a thermodynamic result of the cluster glycoside effect. Our findings, that the surfaces we studied can induce stable initial attachment and influence the ratio of bacterial strains on the surface, may be applied to harness various useful microbial communities." @default.
- W3214083809 created "2021-11-22" @default.
- W3214083809 creator A5011657170 @default.
- W3214083809 date "2020-01-01" @default.
- W3214083809 modified "2023-09-27" @default.
- W3214083809 title "Surface Interactions of Metal-Reducing Bacteria: Attachment and Cellular Appendages" @default.
- W3214083809 hasPublicationYear "2020" @default.
- W3214083809 type Work @default.
- W3214083809 sameAs 3214083809 @default.
- W3214083809 citedByCount "0" @default.
- W3214083809 crossrefType "journal-article" @default.
- W3214083809 hasAuthorship W3214083809A5011657170 @default.
- W3214083809 hasConcept C104317684 @default.
- W3214083809 hasConcept C12554922 @default.
- W3214083809 hasConcept C127413603 @default.
- W3214083809 hasConcept C171250308 @default.
- W3214083809 hasConcept C174832134 @default.
- W3214083809 hasConcept C185592680 @default.
- W3214083809 hasConcept C192562407 @default.
- W3214083809 hasConcept C2776062086 @default.
- W3214083809 hasConcept C2779812452 @default.
- W3214083809 hasConcept C2781170380 @default.
- W3214083809 hasConcept C42360764 @default.
- W3214083809 hasConcept C523546767 @default.
- W3214083809 hasConcept C54355233 @default.
- W3214083809 hasConcept C547475151 @default.
- W3214083809 hasConcept C55493867 @default.
- W3214083809 hasConcept C58123911 @default.
- W3214083809 hasConcept C86803240 @default.
- W3214083809 hasConceptScore W3214083809C104317684 @default.
- W3214083809 hasConceptScore W3214083809C12554922 @default.
- W3214083809 hasConceptScore W3214083809C127413603 @default.
- W3214083809 hasConceptScore W3214083809C171250308 @default.
- W3214083809 hasConceptScore W3214083809C174832134 @default.
- W3214083809 hasConceptScore W3214083809C185592680 @default.
- W3214083809 hasConceptScore W3214083809C192562407 @default.
- W3214083809 hasConceptScore W3214083809C2776062086 @default.
- W3214083809 hasConceptScore W3214083809C2779812452 @default.
- W3214083809 hasConceptScore W3214083809C2781170380 @default.
- W3214083809 hasConceptScore W3214083809C42360764 @default.
- W3214083809 hasConceptScore W3214083809C523546767 @default.
- W3214083809 hasConceptScore W3214083809C54355233 @default.
- W3214083809 hasConceptScore W3214083809C547475151 @default.
- W3214083809 hasConceptScore W3214083809C55493867 @default.
- W3214083809 hasConceptScore W3214083809C58123911 @default.
- W3214083809 hasConceptScore W3214083809C86803240 @default.
- W3214083809 hasLocation W32140838091 @default.
- W3214083809 hasOpenAccess W3214083809 @default.
- W3214083809 hasPrimaryLocation W32140838091 @default.
- W3214083809 hasRelatedWork W1993082401 @default.
- W3214083809 hasRelatedWork W1996732369 @default.
- W3214083809 hasRelatedWork W1997626939 @default.
- W3214083809 hasRelatedWork W2027018343 @default.
- W3214083809 hasRelatedWork W2030799089 @default.
- W3214083809 hasRelatedWork W2034116280 @default.
- W3214083809 hasRelatedWork W2034434000 @default.
- W3214083809 hasRelatedWork W2058128063 @default.
- W3214083809 hasRelatedWork W2064197451 @default.
- W3214083809 hasRelatedWork W2070983493 @default.
- W3214083809 hasRelatedWork W2087593300 @default.
- W3214083809 hasRelatedWork W2094049407 @default.
- W3214083809 hasRelatedWork W2159606063 @default.
- W3214083809 hasRelatedWork W2220068171 @default.
- W3214083809 hasRelatedWork W2734436969 @default.
- W3214083809 hasRelatedWork W2897745154 @default.
- W3214083809 hasRelatedWork W2922853036 @default.
- W3214083809 hasRelatedWork W2978445749 @default.
- W3214083809 hasRelatedWork W563396768 @default.
- W3214083809 hasRelatedWork W2772398221 @default.
- W3214083809 isParatext "false" @default.
- W3214083809 isRetracted "false" @default.
- W3214083809 magId "3214083809" @default.
- W3214083809 workType "article" @default.