Matches in SemOpenAlex for { <https://semopenalex.org/work/W3214340986> ?p ?o ?g. }
- W3214340986 endingPage "1560" @default.
- W3214340986 startingPage "1547" @default.
- W3214340986 abstract "Large-pore channels, including those formed by connexin, pannexin, innexin proteins, are part of a broad family of plasma membrane channels found in vertebrates and invertebrates, which share topology features. Despite their relevance in parasitic diseases such as Chagas and malaria, it was unknown whether these large-pore channels are present in unicellular organisms. We identified 14 putative proteins in Trypanosomatidae parasites as presumptive homologs of innexin proteins. All proteins possess the canonical motif of the innexin family, a pentapeptide YYQWV, and 10 of them share a classical membrane topology of large-pore channels. A sequence similarity network analysis confirmed their closeness to innexin proteins. A bioinformatic model showed that a homolog of Trypanosoma cruzi (T. cruzi) could presumptively form a stable octamer channel with a highly positive electrostatic potential in the internal cavities and extracellular entrance due to the notable predominance of residues such as Arg or Lys. In vitro dye uptake assays showed that divalent cations-free solution increases YO-PRO-1 uptake and hyperosmotic stress increases DAPI uptake in epimastigotes of T. cruzi. Those effects were sensitive to probenecid. Furthermore, probenecid reduced the proliferation and transformation of T. cruzi. Moreover, probenecid or carbenoxolone increased the parasite sensitivity to antiparasitic drugs commonly used in therapy against Chagas. Our study suggests the existence of innexin homologs in unicellular organisms, which could be protein subunits of new large-pore channels in unicellular organisms." @default.
- W3214340986 created "2021-11-22" @default.
- W3214340986 creator A5003269898 @default.
- W3214340986 creator A5007890135 @default.
- W3214340986 creator A5009773603 @default.
- W3214340986 creator A5012811209 @default.
- W3214340986 creator A5013850089 @default.
- W3214340986 creator A5017160286 @default.
- W3214340986 creator A5020213763 @default.
- W3214340986 creator A5023236269 @default.
- W3214340986 creator A5034249443 @default.
- W3214340986 creator A5071312916 @default.
- W3214340986 creator A5077231406 @default.
- W3214340986 creator A5083887530 @default.
- W3214340986 date "2021-11-15" @default.
- W3214340986 modified "2023-10-17" @default.
- W3214340986 title "Unnexins: Homologs of innexin proteins in <i>Trypanosomatidae</i> parasites" @default.
- W3214340986 cites W1756553266 @default.
- W3214340986 cites W1975988897 @default.
- W3214340986 cites W1981225934 @default.
- W3214340986 cites W1993134250 @default.
- W3214340986 cites W1997215245 @default.
- W3214340986 cites W1998287336 @default.
- W3214340986 cites W1999395498 @default.
- W3214340986 cites W2013913567 @default.
- W3214340986 cites W2019015469 @default.
- W3214340986 cites W2022907702 @default.
- W3214340986 cites W2025112823 @default.
- W3214340986 cites W2029582401 @default.
- W3214340986 cites W2029667189 @default.
- W3214340986 cites W2032141731 @default.
- W3214340986 cites W2033701969 @default.
- W3214340986 cites W2043799547 @default.
- W3214340986 cites W2046766809 @default.
- W3214340986 cites W2047399603 @default.
- W3214340986 cites W2049772178 @default.
- W3214340986 cites W2050350002 @default.
- W3214340986 cites W2051367002 @default.
- W3214340986 cites W2055043387 @default.
- W3214340986 cites W2057477511 @default.
- W3214340986 cites W2067174909 @default.
- W3214340986 cites W2068202641 @default.
- W3214340986 cites W2071769324 @default.
- W3214340986 cites W2077857158 @default.
- W3214340986 cites W2090896240 @default.
- W3214340986 cites W2101532349 @default.
- W3214340986 cites W2104244040 @default.
- W3214340986 cites W2104285627 @default.
- W3214340986 cites W2104357869 @default.
- W3214340986 cites W2118233996 @default.
- W3214340986 cites W2123139638 @default.
- W3214340986 cites W2125583308 @default.
- W3214340986 cites W2134566372 @default.
- W3214340986 cites W2137531873 @default.
- W3214340986 cites W2147611167 @default.
- W3214340986 cites W2148998009 @default.
- W3214340986 cites W2151877638 @default.
- W3214340986 cites W2156125289 @default.
- W3214340986 cites W2202578400 @default.
- W3214340986 cites W2212701647 @default.
- W3214340986 cites W2323104785 @default.
- W3214340986 cites W2333694147 @default.
- W3214340986 cites W2404280981 @default.
- W3214340986 cites W2557315911 @default.
- W3214340986 cites W2605068739 @default.
- W3214340986 cites W2620198502 @default.
- W3214340986 cites W2769507762 @default.
- W3214340986 cites W2805363626 @default.
- W3214340986 cites W2891858989 @default.
- W3214340986 cites W2896219490 @default.
- W3214340986 cites W2934069517 @default.
- W3214340986 cites W2938574745 @default.
- W3214340986 cites W3003717091 @default.
- W3214340986 cites W3006000566 @default.
- W3214340986 cites W3013925207 @default.
- W3214340986 cites W3032333420 @default.
- W3214340986 cites W3083297967 @default.
- W3214340986 cites W3101484555 @default.
- W3214340986 cites W3109596145 @default.
- W3214340986 cites W3119013734 @default.
- W3214340986 cites W3123019810 @default.
- W3214340986 cites W3153641279 @default.
- W3214340986 cites W3168867659 @default.
- W3214340986 cites W3199239196 @default.
- W3214340986 doi "https://doi.org/10.1002/jcp.30626" @default.
- W3214340986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34779505" @default.
- W3214340986 hasPublicationYear "2021" @default.
- W3214340986 type Work @default.
- W3214340986 sameAs 3214340986 @default.
- W3214340986 citedByCount "3" @default.
- W3214340986 countsByYear W32143409862023 @default.
- W3214340986 crossrefType "journal-article" @default.
- W3214340986 hasAuthorship W3214340986A5003269898 @default.
- W3214340986 hasAuthorship W3214340986A5007890135 @default.
- W3214340986 hasAuthorship W3214340986A5009773603 @default.
- W3214340986 hasAuthorship W3214340986A5012811209 @default.
- W3214340986 hasAuthorship W3214340986A5013850089 @default.
- W3214340986 hasAuthorship W3214340986A5017160286 @default.