FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3214535932> ?p ?o ?g. }

• W3214535932 endingPage "184" @default.
• W3214535932 startingPage "172" @default.
• W3214535932 abstract "The phenotype of glioma-initiating cells (GIC) is modulated by cell-intrinsic and cell-extrinsic factors. Phenotypic heterogeneity and plasticity of GIC is an important limitation to therapeutic approaches targeting cancer stem cells. Plasticity also presents a challenge to the identification, isolation, and propagation of purified cancer stem cells. Here we use a barcode labelling approach of GIC to generate clonal populations over a number of passages, in combination with phenotyping using the established stem cell markers CD133, CD15, CD44, and A2B5. Using two cell lines derived from isocitrate dehydrogenase (IDH)-wildtype glioblastoma, we identify a remarkable heterogeneity of the phenotypes between the cell lines. During passaging, clonal expansion manifests as the emergence of a limited number of barcoded clones and a decrease in the overall number of clones. Dual-labelled GIC are capable of forming traceable clonal populations which emerge after as few as two passages from mixed cultures and through analyses of similarity of relative proportions of 16 surface markers we were able to pinpoint the fate of such populations. By generating tumour organoids we observed a remarkable persistence of dominant clones but also a significant plasticity of stemness marker expression. Our study presents an experimental approach to simultaneously barcode and phenotype glioma-initiating cells to assess their functional properties, for example to screen newly established GIC for tumour-specific therapeutic vulnerabilities. The authors barcoded glioma-initiating cells (GIC) using combinations of virally encoded fluorophores. GIC show a strong tendency to form clonal populations over as few as two passages. Combined with stem cell marker phenotyping and computational analysis, they could trace the fate of such populations. This model presents an approach for rapid assessment of newly established GIC to assess tumour-specific vulnerabilities." @default.
• W3214535932 created "2021-11-22" @default.
• W3214535932 creator A5000485568 @default.
• W3214535932 creator A5013573764 @default.
• W3214535932 creator A5025165030 @default.
• W3214535932 creator A5028564657 @default.
• W3214535932 creator A5030810824 @default.
• W3214535932 creator A5033226187 @default.
• W3214535932 creator A5050445524 @default.
• W3214535932 creator A5063444030 @default.
• W3214535932 creator A5077148960 @default.
• W3214535932 creator A5087136987 @default.
• W3214535932 date "2022-02-01" @default.
• W3214535932 modified "2023-10-14" @default.
• W3214535932 title "Phenotyping clonal populations of glioma stem cell reveals a high degree of plasticity in response to changes of microenvironment" @default.
• W3214535932 cites W1497918542 @default.
• W3214535932 cites W1526705543 @default.
• W3214535932 cites W1775349404 @default.
• W3214535932 cites W1902577542 @default.
• W3214535932 cites W1977252540 @default.
• W3214535932 cites W1985395763 @default.
• W3214535932 cites W1987885030 @default.
• W3214535932 cites W1989958716 @default.
• W3214535932 cites W1998728654 @default.
• W3214535932 cites W2001544973 @default.
• W3214535932 cites W2016851798 @default.
• W3214535932 cites W2024672608 @default.
• W3214535932 cites W2024705492 @default.
• W3214535932 cites W2027303624 @default.
• W3214535932 cites W2036870885 @default.
• W3214535932 cites W2040458067 @default.
• W3214535932 cites W2054139397 @default.
• W3214535932 cites W2061738719 @default.
• W3214535932 cites W2065737043 @default.
• W3214535932 cites W2073973240 @default.
• W3214535932 cites W2078012053 @default.
• W3214535932 cites W2081377411 @default.
• W3214535932 cites W2089870318 @default.
• W3214535932 cites W2090057452 @default.
• W3214535932 cites W2104195581 @default.
• W3214535932 cites W2128662106 @default.
• W3214535932 cites W2153375936 @default.
• W3214535932 cites W2163024465 @default.
• W3214535932 cites W2168631000 @default.
• W3214535932 cites W2169370347 @default.
• W3214535932 cites W2286284214 @default.
• W3214535932 cites W2363443185 @default.
• W3214535932 cites W2578497428 @default.
• W3214535932 cites W2592950943 @default.
• W3214535932 cites W2752214746 @default.
• W3214535932 cites W2774567558 @default.
• W3214535932 cites W2787816121 @default.
• W3214535932 cites W2807995030 @default.
• W3214535932 cites W2893317708 @default.
• W3214535932 cites W2894102714 @default.
• W3214535932 cites W2905431641 @default.
• W3214535932 cites W2921718105 @default.
• W3214535932 cites W2922989408 @default.
• W3214535932 cites W2936375023 @default.
• W3214535932 cites W2976292707 @default.
• W3214535932 cites W3011002672 @default.
• W3214535932 cites W3039954766 @default.
• W3214535932 cites W3165056209 @default.
• W3214535932 cites W3206837269 @default.
• W3214535932 doi "https://doi.org/10.1038/s41374-021-00695-2" @default.
• W3214535932 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36775406" @default.
• W3214535932 hasPublicationYear "2022" @default.
• W3214535932 type Work @default.
• W3214535932 sameAs 3214535932 @default.
• W3214535932 citedByCount "5" @default.
• W3214535932 countsByYear W32145359322022 @default.
• W3214535932 countsByYear W32145359322023 @default.
• W3214535932 crossrefType "journal-article" @default.
• W3214535932 hasAuthorship W3214535932A5000485568 @default.
• W3214535932 hasAuthorship W3214535932A5013573764 @default.
• W3214535932 hasAuthorship W3214535932A5025165030 @default.
• W3214535932 hasAuthorship W3214535932A5028564657 @default.
• W3214535932 hasAuthorship W3214535932A5030810824 @default.
• W3214535932 hasAuthorship W3214535932A5033226187 @default.
• W3214535932 hasAuthorship W3214535932A5050445524 @default.
• W3214535932 hasAuthorship W3214535932A5063444030 @default.
• W3214535932 hasAuthorship W3214535932A5077148960 @default.
• W3214535932 hasAuthorship W3214535932A5087136987 @default.
• W3214535932 hasBestOaLocation W32145359321 @default.
• W3214535932 hasConcept C104317684 @default.
• W3214535932 hasConcept C113968399 @default.
• W3214535932 hasConcept C121608353 @default.
• W3214535932 hasConcept C127716648 @default.
• W3214535932 hasConcept C136302470 @default.
• W3214535932 hasConcept C1491633281 @default.
• W3214535932 hasConcept C181199279 @default.
• W3214535932 hasConcept C2777150147 @default.
• W3214535932 hasConcept C2778227246 @default.
• W3214535932 hasConcept C2780932548 @default.
• W3214535932 hasConcept C28328180 @default.
• W3214535932 hasConcept C502942594 @default.
• W3214535932 hasConcept C54355233 @default.
• W3214535932 hasConcept C55427017 @default.

• next