Matches in SemOpenAlex for { <https://semopenalex.org/work/W3214573095> ?p ?o ?g. }
- W3214573095 abstract "Extracellular vesicles (EVs) are biological nanoparticles with important roles in intercellular communication, and potential as drug delivery vehicles. Here we demonstrate a role for the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in EV assembly and secretion. We observe high levels of GAPDH binding to the outer surface of EVs via a phosphatidylserine binding motif (G58), which promotes extensive EV clustering. Further studies in a Drosophila EV biogenesis model reveal that GAPDH is required for the normal generation of intraluminal vesicles in endosomal compartments, and promotes vesicle clustering. Fusion of the GAPDH-derived G58 peptide to dsRNA-binding motifs enables highly efficient loading of small interfering RNA (siRNA) onto the EV surface. Such vesicles efficiently deliver siRNA to multiple anatomical regions of the brain in a Huntington's disease mouse model after systemic injection, resulting in silencing of the huntingtin gene in different regions of the brain." @default.
- W3214573095 created "2021-11-22" @default.
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- W3214573095 date "2021-11-18" @default.
- W3214573095 modified "2023-10-17" @default.
- W3214573095 title "GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain" @default.
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- W3214573095 doi "https://doi.org/10.1038/s41467-021-27056-3" @default.
- W3214573095 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8602309" @default.
- W3214573095 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34795295" @default.
- W3214573095 hasPublicationYear "2021" @default.
- W3214573095 type Work @default.