FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3214955087> ?p ?o ?g. }

• W3214955087 abstract "Abstract Synthetic directed evolution via localized sequence diversification and the simultaneous application of selection pressure is a promising method for producing new, beneficial alleles that affect traits of interest in diverse species; however, this technique has rarely been applied in plants. Developing systems to induce localized sequence diversification at high efficiency will expand our ability to evolve traits of interest that improve global food security. In this study, we designed, built, and tested a chimeric fusion of T7 RNA Polymerase (RNAP) and deaminase to enable the localized sequence diversification of a target sequence of interest. We tested our T7 RNAP–DNA base editor in Nicotiana benthamiana transient assays to target a transgene expressing GFP under the control of the T7 promoter. More than 7% of C nucleotides were converted to T in long segments of the GFP sequence. We then targeted the T7 promoter-driven ACETOLACTATE SYNTHASE ( ALS ) sequence that had been stably integrated in the rice ( Oryza sativa ) genome and generated C-to-T and G-to-A transitions. We used herbicide treatment as selection pressure for the evolution of the ALS sequence, resulting in the enrichment of herbicide-responsive residues. We then targeted these herbicide-responsive regions in the rice genome using a CRISPR-directed evolution platform and identified herbicide-resistant ALS variants. Thus, our system could be used for the continuous synthetic evolution of gene functions to produce variants with improved herbicide resistance, as well as for other trait engineering applications." @default.
• W3214955087 created "2021-12-06" @default.
• W3214955087 creator A5015661108 @default.
• W3214955087 creator A5041218190 @default.
• W3214955087 creator A5066747292 @default.
• W3214955087 date "2021-12-01" @default.
• W3214955087 modified "2023-10-15" @default.
• W3214955087 title "Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor" @default.
• W3214955087 cites W1568200847 @default.
• W3214955087 cites W1973721352 @default.
• W3214955087 cites W2010339813 @default.
• W3214955087 cites W2010851273 @default.
• W3214955087 cites W2012598052 @default.
• W3214955087 cites W2015580675 @default.
• W3214955087 cites W2042090583 @default.
• W3214955087 cites W2052024371 @default.
• W3214955087 cites W2062958450 @default.
• W3214955087 cites W2070360032 @default.
• W3214955087 cites W2097861808 @default.
• W3214955087 cites W2141157190 @default.
• W3214955087 cites W2144779133 @default.
• W3214955087 cites W2148302985 @default.
• W3214955087 cites W2161322658 @default.
• W3214955087 cites W2542621333 @default.
• W3214955087 cites W2748541229 @default.
• W3214955087 cites W2766599608 @default.
• W3214955087 cites W2793209087 @default.
• W3214955087 cites W2807173586 @default.
• W3214955087 cites W2807979852 @default.
• W3214955087 cites W2836805751 @default.
• W3214955087 cites W2887001543 @default.
• W3214955087 cites W2897295193 @default.
• W3214955087 cites W2944038963 @default.
• W3214955087 cites W2951630487 @default.
• W3214955087 cites W2952856897 @default.
• W3214955087 cites W2964407041 @default.
• W3214955087 cites W2970289976 @default.
• W3214955087 cites W2979346767 @default.
• W3214955087 cites W2981137429 @default.
• W3214955087 cites W2994724409 @default.
• W3214955087 cites W3002620407 @default.
• W3214955087 cites W3003676829 @default.
• W3214955087 cites W3012327875 @default.
• W3214955087 cites W3025159539 @default.
• W3214955087 cites W3025291808 @default.
• W3214955087 cites W3027322274 @default.
• W3214955087 cites W3030053376 @default.
• W3214955087 cites W3038121973 @default.
• W3214955087 cites W3046580469 @default.
• W3214955087 cites W3093469725 @default.
• W3214955087 cites W3102748721 @default.
• W3214955087 cites W3115176682 @default.
• W3214955087 cites W3119958255 @default.
• W3214955087 cites W3135418591 @default.
• W3214955087 cites W3151309882 @default.
• W3214955087 cites W3158559260 @default.
• W3214955087 cites W3172958496 @default.
• W3214955087 cites W3196437707 @default.
• W3214955087 cites W4211014389 @default.
• W3214955087 doi "https://doi.org/10.1101/2021.11.30.470689" @default.
• W3214955087 hasPublicationYear "2021" @default.
• W3214955087 type Work @default.
• W3214955087 sameAs 3214955087 @default.
• W3214955087 citedByCount "2" @default.
• W3214955087 countsByYear W32149550872022 @default.
• W3214955087 crossrefType "posted-content" @default.
• W3214955087 hasAuthorship W3214955087A5015661108 @default.
• W3214955087 hasAuthorship W3214955087A5041218190 @default.
• W3214955087 hasAuthorship W3214955087A5066747292 @default.
• W3214955087 hasBestOaLocation W32149550871 @default.
• W3214955087 hasConcept C104317684 @default.
• W3214955087 hasConcept C132455925 @default.
• W3214955087 hasConcept C141231307 @default.
• W3214955087 hasConcept C143065580 @default.
• W3214955087 hasConcept C145290725 @default.
• W3214955087 hasConcept C2776441376 @default.
• W3214955087 hasConcept C2780048736 @default.
• W3214955087 hasConcept C54355233 @default.
• W3214955087 hasConcept C547475151 @default.
• W3214955087 hasConcept C70721500 @default.
• W3214955087 hasConcept C86803240 @default.
• W3214955087 hasConcept C9418097 @default.
• W3214955087 hasConcept C98108389 @default.
• W3214955087 hasConceptScore W3214955087C104317684 @default.
• W3214955087 hasConceptScore W3214955087C132455925 @default.
• W3214955087 hasConceptScore W3214955087C141231307 @default.
• W3214955087 hasConceptScore W3214955087C143065580 @default.
• W3214955087 hasConceptScore W3214955087C145290725 @default.
• W3214955087 hasConceptScore W3214955087C2776441376 @default.
• W3214955087 hasConceptScore W3214955087C2780048736 @default.
• W3214955087 hasConceptScore W3214955087C54355233 @default.
• W3214955087 hasConceptScore W3214955087C547475151 @default.
• W3214955087 hasConceptScore W3214955087C70721500 @default.
• W3214955087 hasConceptScore W3214955087C86803240 @default.
• W3214955087 hasConceptScore W3214955087C9418097 @default.
• W3214955087 hasConceptScore W3214955087C98108389 @default.
• W3214955087 hasLocation W32149550871 @default.
• W3214955087 hasLocation W32149550872 @default.
• W3214955087 hasLocation W32149550873 @default.
• W3214955087 hasOpenAccess W3214955087 @default.

• next