Matches in SemOpenAlex for { <https://semopenalex.org/work/W3215335144> ?p ?o ?g. }
- W3215335144 endingPage "78" @default.
- W3215335144 startingPage "69" @default.
- W3215335144 abstract "A lactic acid bacteria (LAB) producing γ-aminobutyric acid (GABA) was isolated from Gat-Kimchi, a Korean vegetable food. The isolate, K285, was identified as Latilactobacillus (formly Lactobacillus) curvatus. The gadB encoding glutamate decarboxylase (GAD) was cloned and an ORF encoding a protein of 451 amino acids was located. K285 GAD was smaller than other LAB GADs, and its amino acid sequence showed less than 80% homology with other LAB GADs, indicating the uniqueness of K285 GAD. The gadC encoding glutamate/GABA antiporter was located 75 bp upstream of gadB, indicating gadCB operon structure. The gadB was overexpressed in Escherichia coli and recombinant GAD was purified. Optimum pH and temperature of recombinant K285 GAD were pH 5.0 and 50 °C, respectively, and the activity was dependent on pyridoxal 5′-phosphate. The Km and Vmax of GAD were 5.35 ± 0.27 mM and 0.041 ± 0.0008 mM/min, respectively. Lb. curvatus K285 might be useful for the production of foods enriched with GABA." @default.
- W3215335144 created "2021-12-06" @default.
- W3215335144 creator A5002829719 @default.
- W3215335144 creator A5013851036 @default.
- W3215335144 creator A5055385854 @default.
- W3215335144 creator A5068344018 @default.
- W3215335144 creator A5071146990 @default.
- W3215335144 creator A5071325470 @default.
- W3215335144 creator A5076636433 @default.
- W3215335144 date "2021-11-21" @default.
- W3215335144 modified "2023-10-18" @default.
- W3215335144 title "Characterization of a novel glutamate decarboxylase (GAD) from Latilactobacillus curvatus K285 isolated from Gat -Kimchi" @default.
- W3215335144 cites W1967153009 @default.
- W3215335144 cites W1967989888 @default.
- W3215335144 cites W1990591017 @default.
- W3215335144 cites W1995374098 @default.
- W3215335144 cites W1996317511 @default.
- W3215335144 cites W2013437045 @default.
- W3215335144 cites W2014289720 @default.
- W3215335144 cites W2033189179 @default.
- W3215335144 cites W2070874563 @default.
- W3215335144 cites W2110321741 @default.
- W3215335144 cites W2113230999 @default.
- W3215335144 cites W2114697338 @default.
- W3215335144 cites W2121363414 @default.
- W3215335144 cites W2155249103 @default.
- W3215335144 cites W2188819145 @default.
- W3215335144 cites W2323448284 @default.
- W3215335144 cites W2326482091 @default.
- W3215335144 cites W2353290092 @default.
- W3215335144 cites W2411627808 @default.
- W3215335144 cites W2577213478 @default.
- W3215335144 cites W2737887791 @default.
- W3215335144 cites W2741449099 @default.
- W3215335144 cites W2748641449 @default.
- W3215335144 cites W2759786703 @default.
- W3215335144 cites W2790625162 @default.
- W3215335144 cites W2996295400 @default.
- W3215335144 cites W3004119871 @default.
- W3215335144 cites W3083839979 @default.
- W3215335144 cites W3107137402 @default.
- W3215335144 doi "https://doi.org/10.1007/s10068-021-01005-8" @default.
- W3215335144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35059231" @default.
- W3215335144 hasPublicationYear "2021" @default.
- W3215335144 type Work @default.
- W3215335144 sameAs 3215335144 @default.
- W3215335144 citedByCount "3" @default.
- W3215335144 countsByYear W32153351442022 @default.
- W3215335144 countsByYear W32153351442023 @default.
- W3215335144 crossrefType "journal-article" @default.
- W3215335144 hasAuthorship W3215335144A5002829719 @default.
- W3215335144 hasAuthorship W3215335144A5013851036 @default.
- W3215335144 hasAuthorship W3215335144A5055385854 @default.
- W3215335144 hasAuthorship W3215335144A5068344018 @default.
- W3215335144 hasAuthorship W3215335144A5071146990 @default.
- W3215335144 hasAuthorship W3215335144A5071325470 @default.
- W3215335144 hasAuthorship W3215335144A5076636433 @default.
- W3215335144 hasBestOaLocation W32153351442 @default.
- W3215335144 hasConcept C104317684 @default.
- W3215335144 hasConcept C181199279 @default.
- W3215335144 hasConcept C185592680 @default.
- W3215335144 hasConcept C203075996 @default.
- W3215335144 hasConcept C2775920511 @default.
- W3215335144 hasConcept C40767141 @default.
- W3215335144 hasConcept C515207424 @default.
- W3215335144 hasConcept C523546767 @default.
- W3215335144 hasConcept C54355233 @default.
- W3215335144 hasConcept C547475151 @default.
- W3215335144 hasConcept C55493867 @default.
- W3215335144 hasConcept C86803240 @default.
- W3215335144 hasConcept C9497952 @default.
- W3215335144 hasConceptScore W3215335144C104317684 @default.
- W3215335144 hasConceptScore W3215335144C181199279 @default.
- W3215335144 hasConceptScore W3215335144C185592680 @default.
- W3215335144 hasConceptScore W3215335144C203075996 @default.
- W3215335144 hasConceptScore W3215335144C2775920511 @default.
- W3215335144 hasConceptScore W3215335144C40767141 @default.
- W3215335144 hasConceptScore W3215335144C515207424 @default.
- W3215335144 hasConceptScore W3215335144C523546767 @default.
- W3215335144 hasConceptScore W3215335144C54355233 @default.
- W3215335144 hasConceptScore W3215335144C547475151 @default.
- W3215335144 hasConceptScore W3215335144C55493867 @default.
- W3215335144 hasConceptScore W3215335144C86803240 @default.
- W3215335144 hasConceptScore W3215335144C9497952 @default.
- W3215335144 hasFunder F4320321318 @default.
- W3215335144 hasFunder F4320321408 @default.
- W3215335144 hasFunder F4320322030 @default.
- W3215335144 hasIssue "1" @default.
- W3215335144 hasLocation W32153351441 @default.
- W3215335144 hasLocation W32153351442 @default.
- W3215335144 hasLocation W32153351443 @default.
- W3215335144 hasOpenAccess W3215335144 @default.
- W3215335144 hasPrimaryLocation W32153351441 @default.
- W3215335144 hasRelatedWork W1566821346 @default.
- W3215335144 hasRelatedWork W1920918052 @default.
- W3215335144 hasRelatedWork W1997359581 @default.
- W3215335144 hasRelatedWork W229129424 @default.
- W3215335144 hasRelatedWork W2378190089 @default.