FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3215668868> ?p ?o ?g. }

• W3215668868 endingPage "300" @default.
• W3215668868 startingPage "286" @default.
• W3215668868 abstract "<b><i>Background:</i></b> Tympanic membrane perforations (TMPs) are a common complication of trauma and infection. Persisting perforations result from the unique location of the tympanic membrane. The wound is surrounded by air of the middle ear and the external auditory canal. The inadequate wound bed, growth factor, and blood supply lead to circular epithelialization of the perforation’s edge and premature interruption of defect closure. Orthotopic animal models use mechanical or chemical tympanic membrane laceration to identify bioactive wound dressings and overcome premature epithelialization. However, all orthotopic models essentially lack repetitive visualization of the biomaterial-wound interface. Therefore, recent progress in 3D printing of customized wound dressings has not yet been transferred to the unique wound setup of the TMP. Here, we present a novel application for the mice dorsal skinfold chamber (DSC) with an epithelialized full-thickness defect as TMP model. <b><i>Methods:</i></b> A circular 2-mm defect was cut into the extended dorsal skinfold using a biopsy punch. The skinfold was either perforated through both skin layers without prior preparation or perforated on 1 side, following resection of the opposing skin layer. In both groups, the wound was sealed with a coverslip or left unclosed (<i>n</i> = 4). All animals were examined for epithelialization of the edge (histology), size of the perforation (planimetry), neovascularization (repetitive intravital fluorescence microscopy), and inflammation (immunohistology). <b><i>Results:</i></b> The edge of the perforation was overgrown by the cornified squamous epithelium in all pre­parations. Reduction in the perforation’s size was enhanced by application of a coverslip. Microsurgical preparation before biopsy punch perforation and sealing with a coverslip enabled repetitive high-quality intravital fluorescence microscopy. However, spontaneous reduction of the perforation occurred frequently. Therefore, the direct biopsy punch perforation without microsurgical preparation was favorable: spontaneous reduction did not occur throughout 21 days. Moreover, the visualization of the neovascularization was sufficient in intravital microscopy. <b><i>Conclusions:</i></b> The DSC full-thickness defect is a valuable supplement to orthotopic TMP models. Repetitive intravital microscopy of the epithelialized edge enables investigation of the underlying pathophysiology during the transition from the inflammation to the proliferation phase of wound healing. Using established analysis procedures, the present model provides an effective platform for the screening of bioactive materials and transferring progress in tissue engineering to the special conditions of tympanic membrane wound healing." @default.
• W3215668868 created "2021-12-06" @default.
• W3215668868 creator A5007054663 @default.
• W3215668868 creator A5037315050 @default.
• W3215668868 creator A5040080340 @default.
• W3215668868 creator A5041569076 @default.
• W3215668868 creator A5081747456 @default.
• W3215668868 creator A5085746861 @default.
• W3215668868 creator A5088286580 @default.
• W3215668868 date "2021-12-02" @default.
• W3215668868 modified "2023-09-26" @default.
• W3215668868 title "The Dorsal Skinfold Chamber as a New Tympanic Membrane Wound Healing Model: Intravital Insights into the Pathophysiology of Epithelialized Wounds" @default.
• W3215668868 cites W182566489 @default.
• W3215668868 cites W1895058398 @default.
• W3215668868 cites W1905065343 @default.
• W3215668868 cites W1966698595 @default.
• W3215668868 cites W1968696045 @default.
• W3215668868 cites W1975876744 @default.
• W3215668868 cites W1977877161 @default.
• W3215668868 cites W1978158011 @default.
• W3215668868 cites W1982736932 @default.
• W3215668868 cites W1987460218 @default.
• W3215668868 cites W1994644438 @default.
• W3215668868 cites W2004319763 @default.
• W3215668868 cites W2006904739 @default.
• W3215668868 cites W2008863925 @default.
• W3215668868 cites W2015568107 @default.
• W3215668868 cites W2015908052 @default.
• W3215668868 cites W2018318382 @default.
• W3215668868 cites W2023213268 @default.
• W3215668868 cites W2025213801 @default.
• W3215668868 cites W2025434419 @default.
• W3215668868 cites W2028559098 @default.
• W3215668868 cites W2029497643 @default.
• W3215668868 cites W2030364670 @default.
• W3215668868 cites W2043051974 @default.
• W3215668868 cites W2047380841 @default.
• W3215668868 cites W2051680555 @default.
• W3215668868 cites W2051746941 @default.
• W3215668868 cites W2055731941 @default.
• W3215668868 cites W2057305611 @default.
• W3215668868 cites W2062501390 @default.
• W3215668868 cites W2068161514 @default.
• W3215668868 cites W2083644239 @default.
• W3215668868 cites W2087705984 @default.
• W3215668868 cites W2104124295 @default.
• W3215668868 cites W2109725029 @default.
• W3215668868 cites W2136387446 @default.
• W3215668868 cites W2147033622 @default.
• W3215668868 cites W2162140499 @default.
• W3215668868 cites W2169456050 @default.
• W3215668868 cites W2179369986 @default.
• W3215668868 cites W2300267837 @default.
• W3215668868 cites W2316570507 @default.
• W3215668868 cites W2353982544 @default.
• W3215668868 cites W2468045479 @default.
• W3215668868 cites W2494365712 @default.
• W3215668868 cites W2525617781 @default.
• W3215668868 cites W2549698523 @default.
• W3215668868 cites W2555818593 @default.
• W3215668868 cites W2566254651 @default.
• W3215668868 cites W2566399670 @default.
• W3215668868 cites W2614072937 @default.
• W3215668868 cites W2735031962 @default.
• W3215668868 cites W2735611319 @default.
• W3215668868 cites W2737952475 @default.
• W3215668868 cites W2739301921 @default.
• W3215668868 cites W2742997191 @default.
• W3215668868 cites W2744531704 @default.
• W3215668868 cites W2766218430 @default.
• W3215668868 cites W2772570234 @default.
• W3215668868 cites W2781430268 @default.
• W3215668868 cites W2793024840 @default.
• W3215668868 cites W2892400597 @default.
• W3215668868 cites W2899723461 @default.
• W3215668868 cites W2904977573 @default.
• W3215668868 cites W2944267227 @default.
• W3215668868 cites W2953778799 @default.
• W3215668868 cites W2975896651 @default.
• W3215668868 cites W2998848738 @default.
• W3215668868 cites W3006747092 @default.
• W3215668868 cites W3007428796 @default.
• W3215668868 cites W3103366903 @default.
• W3215668868 cites W3205239647 @default.
• W3215668868 doi "https://doi.org/10.1159/000519774" @default.
• W3215668868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34856545" @default.
• W3215668868 hasPublicationYear "2021" @default.
• W3215668868 type Work @default.
• W3215668868 sameAs 3215668868 @default.
• W3215668868 citedByCount "0" @default.
• W3215668868 crossrefType "journal-article" @default.
• W3215668868 hasAuthorship W3215668868A5007054663 @default.
• W3215668868 hasAuthorship W3215668868A5037315050 @default.
• W3215668868 hasAuthorship W3215668868A5040080340 @default.
• W3215668868 hasAuthorship W3215668868A5041569076 @default.
• W3215668868 hasAuthorship W3215668868A5081747456 @default.
• W3215668868 hasAuthorship W3215668868A5085746861 @default.
• W3215668868 hasAuthorship W3215668868A5088286580 @default.

• next