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- W3215702218 abstract "Proximity ligation assay (PLA), also referred to as Duolink® PLA technology, permits detection of protein-protein interactions in situ (<40 nm distance) at endogenous protein levels. It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and takes advantage of specific DNA primers covalently linked to the antibodies. A hybridization step followed by a PCR amplification with fluorescent probes then permits visualization of spots of proximity by fluorescence microscopy." @default.
- W3215702218 created "2021-12-06" @default.
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- W3215702218 date "2021-12-03" @default.
- W3215702218 modified "2023-10-18" @default.
- W3215702218 title "Proximity Ligation Assay (PLA)" @default.
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- W3215702218 doi "https://doi.org/10.1007/978-1-0716-1948-3_13" @default.
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