FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3215813426> ?p ?o ?g. }

• W3215813426 endingPage "127845" @default.
• W3215813426 startingPage "127845" @default.
• W3215813426 abstract "Pollution of N-methyl carbamate (NMC) pesticides is threatening the non-target organisms' survival. Thus, broad-specific antibodies and class-selective immunoassays are demanding for multiple NMCs determination. In this study, we employed a molecular docking-based virtual screening strategy to fast profile antibody spectrum, based on a designed chemical pool containing 17 compounds. A monoclonal antibody (mAb)-6G against carbofuran was used as the objective. The recombinant full-length IgG was successfully expressed to validate the antibody sequences for homology modeling. After docking, we manually categorized the antibody-chemical binding strength into three groups. Non-competitive surface plasmon resonance (SPR) demonstrated the mAb-6G affinitive binding toward five NMCs (carbofuran, isoprocarb, propoxur, carbaryl and carbosulfan), which were classified into strong and moderate binding categories. Antibody binding properties were confirmed again by ic-ELISA and lateral flow immunochromatographic strip. Subsequently, an ultrasensitive indirect competitive fluoromicrosphere-based immunoassay (ic-FMIA) was established with the IC50 (half-maximal inhibitory concentration) values of 0.08-3.37 ng/mL. This portable assay presented a 30-230-fold improved sensitivity than traditional ic-ELISA and was applied in European surface water analysis. Overall, our work provides an efficient platform integrating in-silico and experimental methodologies to accelerate the characterization of hapten-specific antibody binding properties and the development of high-sensitive immunoassays for multi-pollutants monitoring." @default.
• W3215813426 created "2021-12-06" @default.
• W3215813426 creator A5004843894 @default.
• W3215813426 creator A5009662825 @default.
• W3215813426 creator A5020819058 @default.
• W3215813426 creator A5039009774 @default.
• W3215813426 creator A5055316339 @default.
• W3215813426 creator A5062330303 @default.
• W3215813426 creator A5063169050 @default.
• W3215813426 creator A5069183335 @default.
• W3215813426 creator A5087188264 @default.
• W3215813426 date "2022-03-01" @default.
• W3215813426 modified "2023-10-03" @default.
• W3215813426 title "Computer-aided profiling of a unique broad-specific antibody and its application to an ultrasensitive fluoroimmunoassay for five N-methyl carbamate pesticides" @default.
• W3215813426 cites W1986240377 @default.
• W3215813426 cites W1986565761 @default.
• W3215813426 cites W1994014218 @default.
• W3215813426 cites W1999574236 @default.
• W3215813426 cites W2007979904 @default.
• W3215813426 cites W2008009746 @default.
• W3215813426 cites W2013575501 @default.
• W3215813426 cites W2038262006 @default.
• W3215813426 cites W2038994439 @default.
• W3215813426 cites W2056338182 @default.
• W3215813426 cites W2058845914 @default.
• W3215813426 cites W2072131420 @default.
• W3215813426 cites W2081029461 @default.
• W3215813426 cites W2085235150 @default.
• W3215813426 cites W2121310268 @default.
• W3215813426 cites W2124146610 @default.
• W3215813426 cites W2143535624 @default.
• W3215813426 cites W2148516422 @default.
• W3215813426 cites W2307778533 @default.
• W3215813426 cites W2318339982 @default.
• W3215813426 cites W2324110453 @default.
• W3215813426 cites W2566982980 @default.
• W3215813426 cites W2769908751 @default.
• W3215813426 cites W2790826649 @default.
• W3215813426 cites W2794109271 @default.
• W3215813426 cites W2809868937 @default.
• W3215813426 cites W2891992520 @default.
• W3215813426 cites W2894080265 @default.
• W3215813426 cites W2918568639 @default.
• W3215813426 cites W2922263768 @default.
• W3215813426 cites W2964701861 @default.
• W3215813426 cites W2973804254 @default.
• W3215813426 cites W2980123002 @default.
• W3215813426 cites W3010016408 @default.
• W3215813426 cites W3019759883 @default.
• W3215813426 cites W3036832912 @default.
• W3215813426 cites W3080576564 @default.
• W3215813426 cites W3087129549 @default.
• W3215813426 cites W3097369531 @default.
• W3215813426 cites W3129157487 @default.
• W3215813426 cites W3131221963 @default.
• W3215813426 cites W3139371790 @default.
• W3215813426 cites W3166247324 @default.
• W3215813426 doi "https://doi.org/10.1016/j.jhazmat.2021.127845" @default.
• W3215813426 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34865894" @default.
• W3215813426 hasPublicationYear "2022" @default.
• W3215813426 type Work @default.
• W3215813426 sameAs 3215813426 @default.
• W3215813426 citedByCount "8" @default.
• W3215813426 countsByYear W32158134262022 @default.
• W3215813426 countsByYear W32158134262023 @default.
• W3215813426 crossrefType "journal-article" @default.
• W3215813426 hasAuthorship W3215813426A5004843894 @default.
• W3215813426 hasAuthorship W3215813426A5009662825 @default.
• W3215813426 hasAuthorship W3215813426A5020819058 @default.
• W3215813426 hasAuthorship W3215813426A5039009774 @default.
• W3215813426 hasAuthorship W3215813426A5055316339 @default.
• W3215813426 hasAuthorship W3215813426A5062330303 @default.
• W3215813426 hasAuthorship W3215813426A5063169050 @default.
• W3215813426 hasAuthorship W3215813426A5069183335 @default.
• W3215813426 hasAuthorship W3215813426A5087188264 @default.
• W3215813426 hasConcept C106847996 @default.
• W3215813426 hasConcept C155672457 @default.
• W3215813426 hasConcept C159110408 @default.
• W3215813426 hasConcept C159654299 @default.
• W3215813426 hasConcept C161176658 @default.
• W3215813426 hasConcept C171250308 @default.
• W3215813426 hasConcept C185592680 @default.
• W3215813426 hasConcept C192562407 @default.
• W3215813426 hasConcept C203014093 @default.
• W3215813426 hasConcept C2776051353 @default.
• W3215813426 hasConcept C2777365859 @default.
• W3215813426 hasConcept C2779303437 @default.
• W3215813426 hasConcept C2780420688 @default.
• W3215813426 hasConcept C2780862241 @default.
• W3215813426 hasConcept C29144244 @default.
• W3215813426 hasConcept C41685203 @default.
• W3215813426 hasConcept C43617362 @default.
• W3215813426 hasConcept C542903549 @default.
• W3215813426 hasConcept C55493867 @default.
• W3215813426 hasConcept C6557445 @default.
• W3215813426 hasConcept C71924100 @default.
• W3215813426 hasConcept C86803240 @default.
• W3215813426 hasConceptScore W3215813426C106847996 @default.

• next