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- W3216408088 abstract "Glucosinolates (GSLs), secondary metabolites synthesized by cruciferous plants, can be hydrolyzed by myrosinase into compounds, such as isothiocyanates (ITCs), with various bioactivities. Thus, myrosinase plays an important role in the utilization of GSLs. We isolated a bacterial strain, which was identified as Leclercia adecarboxylata, from the rhizosphere soil of rape seedlings and identified two myrosinase genes and an ITC hydrolase gene. Both myrosinases are intracellular and have 658 amino acid residues. Via molecular docking and chemical modification assays investigating the active sites of the myrosinases, arginine was found to be essential for their catalytic activity. Transcriptomic analysis of the response to sinigrin revealed significant up-regulation of some genes involved in allyl-ITC detoxification, with metallo-β-lactamase 3836 having the highest fold change. Thus, we discovered two myrosinases from L. adecarboxylata and demonstrated that the mechanism of tolerance of the bacterium to allyl-ITC likely involved metallo-β-lactamase activity." @default.
- W3216408088 created "2021-12-06" @default.
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- W3216408088 date "2021-11-22" @default.
- W3216408088 modified "2023-09-26" @default.
- W3216408088 title "Identification of Two Myrosinases from a <i>Leclercia adecarboxylata</i> Strain and Investigation of Its Tolerance Mechanism to Glucosinolate Hydrolysate" @default.
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- W3216408088 doi "https://doi.org/10.1021/acs.jafc.1c05285" @default.
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