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• W3216503361 abstract "This study explored the regulation of photosystem and central carbon metabolism in cell growth and fucoxanthin accumulation of Isochrysis zhangjiangensis via transcriptome analysis, targeted metabolite measurements, and flux balance analysis. High light promoted biomass accumulation but dramatically decreased fucoxanthin productivity. It suppressed the active photosystem and reduced chlorophyll content, but improved metabolic flux of Calvin-Benson-Bassham and tricarboxylic acid cycle for massive biomass accumulation. The CO2 fixation was largely dependent on mitochondrial energy illustrated by the integrated metabolic tools. At a molecular level, glyceraldehyde-3-phosphate, acetyl-CoA, and pyruvate contents increased at exponential phase under high light, which tended to participate into fatty acid biosynthesis by the up-regulated ACCase. However, high light inhibited most genes involved in fucoxanthin biosynthesis and induced diadinoxanthin cycle to diatoxanthin form. Therefore, constant light at 100 μmol m-2 s-1 balancing biomass concentration and fucoxanthin content provided the highest fucoxanthin productivity at 3.06 mg L-1 d-1." @default.
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• W3216503361 date "2022-03-01" @default.
• W3216503361 modified "2023-10-14" @default.
• W3216503361 title "Integrated metabolic tools reveal carbon alternative in Isochrysis zhangjiangensis for fucoxanthin improvement" @default.
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• W3216503361 doi "https://doi.org/10.1016/j.biortech.2021.126401" @default.
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