Matches in SemOpenAlex for { <https://semopenalex.org/work/W3216684887> ?p ?o ?g. }
- W3216684887 endingPage "127936" @default.
- W3216684887 startingPage "127936" @default.
- W3216684887 abstract "Antibiotic resistance is considered one of the biggest threats to public health and has become a major concern for governments and international organizations. Combating this problem starts with improving global surveillance of antibiotic resistance genes (ARGs) and applying standardized protocols, both in a clinical and environmental context, in agreement with the One Health approach. Exceptional efforts should be directed to controlling ARGs conferring resistance to Critically Important Antimicrobials (CIA). In this study, a systematic literature review to synthesize data on the identification of mcr genes using a PCR technique was performed. Additionally, a novel set of PCR primers for mcr-1 - mcr-9 genes detection was proposed. The developed primers were in silico and experimentally validated by comparison with mcr-specific PCR primers reported in the literature. This validation, besides being a proof-of-concept for primers' usefulness, provided insight into the distribution of mcr genes in municipal wastewater, clay and river sediments, glacier moraine, manure, seagulls and auks feces and daphnids from four countries. This analysis proved that commonly used primers may deliver false results, and some mcr genes may be overlooked in tested samples. Newly-developed PCR primers turned out to be relevant for the screening of mcr genes in various environments." @default.
- W3216684887 created "2021-12-06" @default.
- W3216684887 creator A5011634670 @default.
- W3216684887 creator A5022701659 @default.
- W3216684887 creator A5024185559 @default.
- W3216684887 creator A5027173915 @default.
- W3216684887 creator A5050919381 @default.
- W3216684887 creator A5052110063 @default.
- W3216684887 creator A5056032702 @default.
- W3216684887 creator A5060925092 @default.
- W3216684887 creator A5062563197 @default.
- W3216684887 creator A5089056757 @default.
- W3216684887 date "2022-03-01" @default.
- W3216684887 modified "2023-10-01" @default.
- W3216684887 title "Development and validation of novel PCR primers for identification of plasmid-mediated colistin resistance (mcr) genes in various environmental settings" @default.
- W3216684887 cites W2028695285 @default.
- W3216684887 cites W2055043387 @default.
- W3216684887 cites W2095724872 @default.
- W3216684887 cites W2113410040 @default.
- W3216684887 cites W2115996594 @default.
- W3216684887 cites W2118537438 @default.
- W3216684887 cites W2125910575 @default.
- W3216684887 cites W2164032940 @default.
- W3216684887 cites W2179036997 @default.
- W3216684887 cites W2323503745 @default.
- W3216684887 cites W2340388742 @default.
- W3216684887 cites W2345854975 @default.
- W3216684887 cites W2462066152 @default.
- W3216684887 cites W2487494087 @default.
- W3216684887 cites W2499077378 @default.
- W3216684887 cites W2526042311 @default.
- W3216684887 cites W2539523900 @default.
- W3216684887 cites W2598427216 @default.
- W3216684887 cites W2734463966 @default.
- W3216684887 cites W2740568892 @default.
- W3216684887 cites W2745113680 @default.
- W3216684887 cites W2754815840 @default.
- W3216684887 cites W2763570542 @default.
- W3216684887 cites W2785542514 @default.
- W3216684887 cites W2789500779 @default.
- W3216684887 cites W2794623497 @default.
- W3216684887 cites W2796584644 @default.
- W3216684887 cites W2799424312 @default.
- W3216684887 cites W2801149479 @default.
- W3216684887 cites W2810427827 @default.
- W3216684887 cites W2881351240 @default.
- W3216684887 cites W2888219047 @default.
- W3216684887 cites W2894424905 @default.
- W3216684887 cites W2944063377 @default.
- W3216684887 cites W2950125070 @default.
- W3216684887 cites W2973994356 @default.
- W3216684887 cites W2980364089 @default.
- W3216684887 cites W2980433385 @default.
- W3216684887 cites W3007806877 @default.
- W3216684887 cites W4234797890 @default.
- W3216684887 cites W4294215472 @default.
- W3216684887 doi "https://doi.org/10.1016/j.jhazmat.2021.127936" @default.
- W3216684887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34883371" @default.
- W3216684887 hasPublicationYear "2022" @default.
- W3216684887 type Work @default.
- W3216684887 sameAs 3216684887 @default.
- W3216684887 citedByCount "4" @default.
- W3216684887 countsByYear W32166848872023 @default.
- W3216684887 crossrefType "journal-article" @default.
- W3216684887 hasAuthorship W3216684887A5011634670 @default.
- W3216684887 hasAuthorship W3216684887A5022701659 @default.
- W3216684887 hasAuthorship W3216684887A5024185559 @default.
- W3216684887 hasAuthorship W3216684887A5027173915 @default.
- W3216684887 hasAuthorship W3216684887A5050919381 @default.
- W3216684887 hasAuthorship W3216684887A5052110063 @default.
- W3216684887 hasAuthorship W3216684887A5056032702 @default.
- W3216684887 hasAuthorship W3216684887A5060925092 @default.
- W3216684887 hasAuthorship W3216684887A5062563197 @default.
- W3216684887 hasAuthorship W3216684887A5089056757 @default.
- W3216684887 hasBestOaLocation W32166848871 @default.
- W3216684887 hasConcept C104317684 @default.
- W3216684887 hasConcept C116834253 @default.
- W3216684887 hasConcept C130217890 @default.
- W3216684887 hasConcept C150903083 @default.
- W3216684887 hasConcept C151730666 @default.
- W3216684887 hasConcept C18903297 @default.
- W3216684887 hasConcept C22744801 @default.
- W3216684887 hasConcept C2775905019 @default.
- W3216684887 hasConcept C2777620836 @default.
- W3216684887 hasConcept C2778910516 @default.
- W3216684887 hasConcept C2779259085 @default.
- W3216684887 hasConcept C2779343474 @default.
- W3216684887 hasConcept C2779969263 @default.
- W3216684887 hasConcept C501593827 @default.
- W3216684887 hasConcept C54355233 @default.
- W3216684887 hasConcept C547475151 @default.
- W3216684887 hasConcept C70721500 @default.
- W3216684887 hasConcept C86803240 @default.
- W3216684887 hasConcept C89423630 @default.
- W3216684887 hasConcept C94665300 @default.
- W3216684887 hasConceptScore W3216684887C104317684 @default.
- W3216684887 hasConceptScore W3216684887C116834253 @default.
- W3216684887 hasConceptScore W3216684887C130217890 @default.