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• W3216719515 abstract "This study aimed to provide efficient recognition of bacterial strains on personal computers from MinION (Nanopore) long read data. Thanks to the fall in sequencing costs, the identification of bacteria can now proceed by whole genome sequencing. MinION is a fast, but highly error-prone sequencing device and it is a challenge to successfully identify the strain content of unknown simple or complex microbial samples. It is heavily constrained by memory management and fast access to the read and genome fragments. Our strategy involves three steps: indexing of known genomic sequences for a given or several bacterial species; a request process to assign a read to a strain by matching it to the closest reference genomes; and a final step looking for a minimum set of strains that best explains the observed reads. We have applied our method, called ORI , on 77 strains of Streptococcus thermophilus . We worked on several genomic distances and obtained a detailed classification of the strains, together with a criterion that allows merging of what we termed ‘sibling’ strains, only separated by a few mutations. Overall, isolated strains can be safely recognized from MinION data. For mixtures of several non-sibling strains, results depend on strain abundance." @default.
• W3216719515 created "2021-12-06" @default.
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• W3216719515 date "2021-11-23" @default.
• W3216719515 modified "2023-10-06" @default.
• W3216719515 title "Identification of isolated or mixed strains from long reads: a challenge met on Streptococcus thermophilus using a MinION sequencer" @default.
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• W3216719515 doi "https://doi.org/10.1099/mgen.0.000654" @default.
• W3216719515 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34812718" @default.
• W3216719515 hasPublicationYear "2021" @default.
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