Matches in SemOpenAlex for { <https://semopenalex.org/work/W3217033189> ?p ?o ?g. }
- W3217033189 abstract "Prokaryotes have evolutionarily acquired an immune system to fend off invading mobile genetic elements, including viral phages and plasmids. Through recognizing specific sequences of the invading nucleic acid, prokaryotes mediate a subsequent degradation process collectively referred to as the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) (CRISPR-Cas) system. The CRISPR-Cas systems are divided into two main classes depending on the structure of the effector Cas proteins. Class I systems have effector modules consisting of multiple proteins, while class II systems have a single multidomain effector. Additionally, the CRISPR-Cas systems can also be categorized into types depending on the spacer acquisition components and their evolutionary features, namely, types I-VI. Among CRISPR/Cas systems, Cas9 is one of the most common multidomain nucleases that identify, degrade, and modulate DNA. Importantly, variants of Cas proteins have recently been found to target RNA, especially the single-effector Cas13 nucleases. The Cas13 has revolutionized our ability to study and perturb RNAs in endogenous microenvironments. The Cas13 effectors offer an excellent candidate for developing novel research tools in virological and biotechnological fields. Herein, in this review, we aim to provide a comprehensive summary of the recent advances of Cas13s for targeting viral RNA for either RNA-mediated degradation or CRISPR-Cas13-based diagnostics. Additionally, we aim to provide an overview of the proposed applications that could revolutionize our understanding of viral-host interactions using Cas13-mediated approaches." @default.
- W3217033189 created "2021-12-06" @default.
- W3217033189 creator A5071872323 @default.
- W3217033189 creator A5087823971 @default.
- W3217033189 date "2021-11-26" @default.
- W3217033189 modified "2023-09-29" @default.
- W3217033189 title "Potential Use of CRISPR/Cas13 Machinery in Understanding Virus–Host Interaction" @default.
- W3217033189 cites W1215958058 @default.
- W3217033189 cites W1510487586 @default.
- W3217033189 cites W1923260397 @default.
- W3217033189 cites W1967046461 @default.
- W3217033189 cites W1969544212 @default.
- W3217033189 cites W1977641557 @default.
- W3217033189 cites W1983059952 @default.
- W3217033189 cites W1996065891 @default.
- W3217033189 cites W2005475183 @default.
- W3217033189 cites W2021837455 @default.
- W3217033189 cites W2044167113 @default.
- W3217033189 cites W2045435533 @default.
- W3217033189 cites W2052192736 @default.
- W3217033189 cites W2077190448 @default.
- W3217033189 cites W2105656400 @default.
- W3217033189 cites W2115569454 @default.
- W3217033189 cites W2116534141 @default.
- W3217033189 cites W2120229820 @default.
- W3217033189 cites W2120503144 @default.
- W3217033189 cites W2123965484 @default.
- W3217033189 cites W2125065061 @default.
- W3217033189 cites W2127096557 @default.
- W3217033189 cites W2129382275 @default.
- W3217033189 cites W2129870871 @default.
- W3217033189 cites W2137450096 @default.
- W3217033189 cites W2143412165 @default.
- W3217033189 cites W2153552126 @default.
- W3217033189 cites W2160686164 @default.
- W3217033189 cites W2161606262 @default.
- W3217033189 cites W2165506056 @default.
- W3217033189 cites W2169244146 @default.
- W3217033189 cites W2176976665 @default.
- W3217033189 cites W2194181707 @default.
- W3217033189 cites W2253584908 @default.
- W3217033189 cites W2260024370 @default.
- W3217033189 cites W2260201183 @default.
- W3217033189 cites W2269848625 @default.
- W3217033189 cites W2274882167 @default.
- W3217033189 cites W2299638516 @default.
- W3217033189 cites W2398597893 @default.
- W3217033189 cites W2409671300 @default.
- W3217033189 cites W2410066853 @default.
- W3217033189 cites W2411891913 @default.
- W3217033189 cites W2522085729 @default.
- W3217033189 cites W2532646138 @default.
- W3217033189 cites W2545689480 @default.
- W3217033189 cites W2580171608 @default.
- W3217033189 cites W2596889486 @default.
- W3217033189 cites W2606915822 @default.
- W3217033189 cites W2615346513 @default.
- W3217033189 cites W2750995391 @default.
- W3217033189 cites W2763042654 @default.
- W3217033189 cites W2765284830 @default.
- W3217033189 cites W2765351619 @default.
- W3217033189 cites W2766697866 @default.
- W3217033189 cites W2768923949 @default.
- W3217033189 cites W2770207242 @default.
- W3217033189 cites W2785904295 @default.
- W3217033189 cites W2791206743 @default.
- W3217033189 cites W2793359586 @default.
- W3217033189 cites W2800905921 @default.
- W3217033189 cites W2883728704 @default.
- W3217033189 cites W2887749453 @default.
- W3217033189 cites W2888449128 @default.
- W3217033189 cites W2896386549 @default.
- W3217033189 cites W2904133612 @default.
- W3217033189 cites W2907167357 @default.
- W3217033189 cites W2911886513 @default.
- W3217033189 cites W2915260514 @default.
- W3217033189 cites W2917276353 @default.
- W3217033189 cites W2920964100 @default.
- W3217033189 cites W2923130410 @default.
- W3217033189 cites W2949168659 @default.
- W3217033189 cites W2950202527 @default.
- W3217033189 cites W2960584468 @default.
- W3217033189 cites W2973637866 @default.
- W3217033189 cites W2978341767 @default.
- W3217033189 cites W2979487934 @default.
- W3217033189 cites W2987009968 @default.
- W3217033189 cites W2989928744 @default.
- W3217033189 cites W2990436921 @default.
- W3217033189 cites W2991671000 @default.
- W3217033189 cites W2993850601 @default.
- W3217033189 cites W2995081665 @default.
- W3217033189 cites W2999351204 @default.
- W3217033189 cites W2999377081 @default.
- W3217033189 cites W3000157412 @default.
- W3217033189 cites W3003217347 @default.
- W3217033189 cites W3004280078 @default.
- W3217033189 cites W3004935084 @default.
- W3217033189 cites W3016048690 @default.
- W3217033189 cites W3020077371 @default.
- W3217033189 cites W3020907828 @default.