FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3217142502> ?p ?o ?g. }

• W3217142502 abstract "Whole-genome sequencing (WGS) of Mycobacterium tuberculosis (MTB) isolates can be used to get an accurate diagnosis, to guide clinical decision making, to control tuberculosis (TB) and for outbreak investigations. We evaluated the performance of long-read (LR) and/or short-read (SR) sequencing for anti-TB drug-resistance prediction using the TBProfiler and Mykrobe tools, the fraction of genome recovery, assembly accuracies and the robustness of two typing approaches based on core-genome SNP (cgSNP) typing and core-genome multi-locus sequence typing (cgMLST). Most of the discrepancies between phenotypic drug-susceptibility testing (DST) and drug-resistance prediction were observed for the first-line drugs rifampicin, isoniazid, pyrazinamide and ethambutol, mainly with LR sequence data. Resistance prediction to second-line drugs made by both TBProfiler and Mykrobe tools with SR- and LR-sequence data were in complete agreement with phenotypic DST except for one isolate. The SR assemblies were more accurate than the LR assemblies, having significantly ( P <0.05) fewer indels and mismatches per 100 kbp. However, the hybrid and LR assemblies had slightly higher genome fractions. For LR assemblies, Canu followed by Racon, and Medaka polishing was the most accurate approach. The cgSNP approach, based on either reads or assemblies, was more robust than the cgMLST approach, especially for LR sequence data. In conclusion, anti-TB drug-resistance prediction, particularly with only LR sequence data, remains challenging, especially for first-line drugs. In addition, SR assemblies appear more accurate than LR ones, and reproducible phylogeny can be achieved using cgSNP approaches." @default.
• W3217142502 created "2021-12-06" @default.
• W3217142502 creator A5005549586 @default.
• W3217142502 creator A5023107356 @default.
• W3217142502 creator A5023431616 @default.
• W3217142502 creator A5023545883 @default.
• W3217142502 creator A5029356969 @default.
• W3217142502 creator A5047011446 @default.
• W3217142502 creator A5056883692 @default.
• W3217142502 creator A5063179666 @default.
• W3217142502 creator A5063438724 @default.
• W3217142502 creator A5075644721 @default.
• W3217142502 creator A5075796846 @default.
• W3217142502 creator A5076094013 @default.
• W3217142502 creator A5080389668 @default.
• W3217142502 creator A5083910434 @default.
• W3217142502 date "2021-11-26" @default.
• W3217142502 modified "2023-09-23" @default.
• W3217142502 title "Evaluation of whole-genome sequence data analysis approaches for short- and long-read sequencing of Mycobacterium tuberculosis" @default.
• W3217142502 cites W1519765314 @default.
• W3217142502 cites W1966822396 @default.
• W3217142502 cites W1975500098 @default.
• W3217142502 cites W2018083981 @default.
• W3217142502 cites W2046429789 @default.
• W3217142502 cites W2077892389 @default.
• W3217142502 cites W2101478678 @default.
• W3217142502 cites W2107772251 @default.
• W3217142502 cites W2131122045 @default.
• W3217142502 cites W2131271579 @default.
• W3217142502 cites W2137111266 @default.
• W3217142502 cites W2150131459 @default.
• W3217142502 cites W2151409320 @default.
• W3217142502 cites W2158023121 @default.
• W3217142502 cites W2160969485 @default.
• W3217142502 cites W2176621021 @default.
• W3217142502 cites W2179507357 @default.
• W3217142502 cites W2224150270 @default.
• W3217142502 cites W2236299009 @default.
• W3217142502 cites W2562683361 @default.
• W3217142502 cites W2564239943 @default.
• W3217142502 cites W2593459163 @default.
• W3217142502 cites W2606138983 @default.
• W3217142502 cites W2747175821 @default.
• W3217142502 cites W2765220300 @default.
• W3217142502 cites W2766429296 @default.
• W3217142502 cites W2788228074 @default.
• W3217142502 cites W2789843538 @default.
• W3217142502 cites W2795065364 @default.
• W3217142502 cites W2797801294 @default.
• W3217142502 cites W2808611629 @default.
• W3217142502 cites W2885548763 @default.
• W3217142502 cites W2901029329 @default.
• W3217142502 cites W2902271181 @default.
• W3217142502 cites W2919609620 @default.
• W3217142502 cites W2920744809 @default.
• W3217142502 cites W2943288764 @default.
• W3217142502 cites W2950354111 @default.
• W3217142502 cites W2951278111 @default.
• W3217142502 cites W2951825561 @default.
• W3217142502 cites W2952167528 @default.
• W3217142502 cites W2952463453 @default.
• W3217142502 cites W2953078967 @default.
• W3217142502 cites W2963643844 @default.
• W3217142502 cites W2990312921 @default.
• W3217142502 cites W2995834440 @default.
• W3217142502 cites W3007172120 @default.
• W3217142502 cites W3024521832 @default.
• W3217142502 cites W3085526196 @default.
• W3217142502 cites W3092843994 @default.
• W3217142502 cites W4253029581 @default.
• W3217142502 doi "https://doi.org/10.1099/mgen.0.000695" @default.
• W3217142502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34825880" @default.
• W3217142502 hasPublicationYear "2021" @default.
• W3217142502 type Work @default.
• W3217142502 sameAs 3217142502 @default.
• W3217142502 citedByCount "11" @default.
• W3217142502 countsByYear W32171425022022 @default.
• W3217142502 countsByYear W32171425022023 @default.
• W3217142502 crossrefType "journal-article" @default.
• W3217142502 hasAuthorship W3217142502A5005549586 @default.
• W3217142502 hasAuthorship W3217142502A5023107356 @default.
• W3217142502 hasAuthorship W3217142502A5023431616 @default.
• W3217142502 hasAuthorship W3217142502A5023545883 @default.
• W3217142502 hasAuthorship W3217142502A5029356969 @default.
• W3217142502 hasAuthorship W3217142502A5047011446 @default.
• W3217142502 hasAuthorship W3217142502A5056883692 @default.
• W3217142502 hasAuthorship W3217142502A5063179666 @default.
• W3217142502 hasAuthorship W3217142502A5063438724 @default.
• W3217142502 hasAuthorship W3217142502A5075644721 @default.
• W3217142502 hasAuthorship W3217142502A5075796846 @default.
• W3217142502 hasAuthorship W3217142502A5076094013 @default.
• W3217142502 hasAuthorship W3217142502A5080389668 @default.
• W3217142502 hasAuthorship W3217142502A5083910434 @default.
• W3217142502 hasBestOaLocation W32171425021 @default.
• W3217142502 hasConcept C104317684 @default.
• W3217142502 hasConcept C135763542 @default.
• W3217142502 hasConcept C141231307 @default.
• W3217142502 hasConcept C142724271 @default.
• W3217142502 hasConcept C24432333 @default.
• W3217142502 hasConcept C2776228421 @default.

• next