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• W3217144623 endingPage "12746" @default.
• W3217144623 startingPage "12746" @default.
• W3217144623 abstract "The O-antigen is the outermost component of the lipopolysaccharide layer in Gram-negative bacteria, and the variation of O-antigen structure provides the basis for bacterial serological diversity. Here, we determined the O-antigen structure of an Escherichia coli strain, LL004, which is totally different from all of the E. coli serogroups. The tetrasaccharide repeating unit was determined as →4)-β-d-Galp-(1→3)-β-d-GlcpNAc6OAc(~70%)-(1→3)-β-d-GalpA-(1→3)-β-d-GalpNAc-(1→ with monosaccharide analysis and NMR spectra. We also characterized the O-antigen gene cluster of LL004, and sequence analysis showed that it correlated well with the O-antigen structure. Deletion and complementation testing further confirmed its role in O-antigen biosynthesis, and indicated that the O-antigen of LL004 is assembled via the Wzx/Wzy dependent pathway. Our findings, in combination, suggest that LL004 should represent a novel serogroup of E. coli." @default.
• W3217144623 created "2021-12-06" @default.
• W3217144623 creator A5037677450 @default.
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• W3217144623 date "2021-11-25" @default.
• W3217144623 modified "2023-10-10" @default.
• W3217144623 title "Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup" @default.
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• W3217144623 doi "https://doi.org/10.3390/ijms222312746" @default.
• W3217144623 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34884549" @default.
• W3217144623 hasPublicationYear "2021" @default.
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