FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3217233381> ?p ?o ?g. }

• W3217233381 abstract "The increasing ineffectiveness of traditional antibiotics and the rise of multidrug resistant (MDR) bacteria have necessitated the revival of bacteriophage (phage) therapy. However, bacteria might also evolve resistance against phages. Phages and their bacterial hosts coexist in nature, resulting in a continuous coevolutionary competition for survival. We have isolated several clinical strains of Pseudomonas aeruginosa and phages that infect them. Among these, the PIAS (Phage Induced Antibiotic Sensitivity) phage belonging to the Myoviridae family can induce multistep genomic deletion in drug-resistant clinical strains of P. aeruginosa, producing a compromised drug efflux system in the bacterial host. We identified two types of mutant lines in the process: green mutants with SNPs (single nucleotide polymorphisms) and smaller deletions and brown mutants with large (∼250 kbp) genomic deletion. We demonstrated that PIAS used the MexXY-OprM system to initiate the infection. P. aeruginosa clogged PIAS phage infection by either modifying or deleting these receptors. The green mutant gaining phage resistance by SNPs could be overcome by evolved PIASs (E-PIASs) with a mutation in its tail-fiber protein. Characterization of the mutant phages will provide a deeper understanding of phage-host interaction. The coevolutionary process continued with large deletions in the same regions of the bacterial genomes to block the (E-)PIAS infection. These mutants gained phage resistance via either complete loss or substantial modifications of the phage receptor, MexXY-OprM, negating its essential role in antibiotic resistance. In vitro and in vivo studies indicated that combined use of PIAS and antibiotics could effectively inhibit P. aeruginosa growth. The phage can either eradicate bacteria or induce antibiotic sensitivity in MDR-resistant clinical strains. We have explored the potential use of combination therapy as an alternative approach against MDR P. aeruginosa infection." @default.
• W3217233381 created "2021-12-06" @default.
• W3217233381 creator A5013292366 @default.
• W3217233381 creator A5016811174 @default.
• W3217233381 creator A5019003915 @default.
• W3217233381 creator A5039988227 @default.
• W3217233381 creator A5040738134 @default.
• W3217233381 creator A5046597357 @default.
• W3217233381 creator A5048155061 @default.
• W3217233381 creator A5063387718 @default.
• W3217233381 creator A5063970052 @default.
• W3217233381 creator A5069168398 @default.
• W3217233381 creator A5069724554 @default.
• W3217233381 creator A5071861999 @default.
• W3217233381 date "2021-12-02" @default.
• W3217233381 modified "2023-10-17" @default.
• W3217233381 title "Survival Comes at a Cost: A Coevolution of Phage and Its Host Leads to Phage Resistance and Antibiotic Sensitivity of Pseudomonas aeruginosa Multidrug Resistant Strains" @default.
• W3217233381 cites W114564532 @default.
• W3217233381 cites W1837900416 @default.
• W3217233381 cites W1958689324 @default.
• W3217233381 cites W1972381405 @default.
• W3217233381 cites W1979482674 @default.
• W3217233381 cites W1988518277 @default.
• W3217233381 cites W1989262406 @default.
• W3217233381 cites W1993669214 @default.
• W3217233381 cites W1994946132 @default.
• W3217233381 cites W1998566510 @default.
• W3217233381 cites W2003324002 @default.
• W3217233381 cites W2005561077 @default.
• W3217233381 cites W2005621749 @default.
• W3217233381 cites W2019197162 @default.
• W3217233381 cites W2025563664 @default.
• W3217233381 cites W2037106368 @default.
• W3217233381 cites W2063089818 @default.
• W3217233381 cites W2073524246 @default.
• W3217233381 cites W2091030812 @default.
• W3217233381 cites W2096093282 @default.
• W3217233381 cites W2108234281 @default.
• W3217233381 cites W2110162745 @default.
• W3217233381 cites W2116279310 @default.
• W3217233381 cites W2121045604 @default.
• W3217233381 cites W2122673596 @default.
• W3217233381 cites W2124365537 @default.
• W3217233381 cites W2133120177 @default.
• W3217233381 cites W2140848003 @default.
• W3217233381 cites W2141274772 @default.
• W3217233381 cites W2142343447 @default.
• W3217233381 cites W2146276053 @default.
• W3217233381 cites W2148753657 @default.
• W3217233381 cites W2152193238 @default.
• W3217233381 cites W2160443607 @default.
• W3217233381 cites W2168808631 @default.
• W3217233381 cites W2176190761 @default.
• W3217233381 cites W2194181707 @default.
• W3217233381 cites W2232017074 @default.
• W3217233381 cites W2235238101 @default.
• W3217233381 cites W2290768068 @default.
• W3217233381 cites W2309308621 @default.
• W3217233381 cites W2346708074 @default.
• W3217233381 cites W2399110743 @default.
• W3217233381 cites W2407344160 @default.
• W3217233381 cites W2416405911 @default.
• W3217233381 cites W2521744514 @default.
• W3217233381 cites W2521846078 @default.
• W3217233381 cites W2562044150 @default.
• W3217233381 cites W2564226239 @default.
• W3217233381 cites W2587003224 @default.
• W3217233381 cites W2606644876 @default.
• W3217233381 cites W2756411496 @default.
• W3217233381 cites W2789876988 @default.
• W3217233381 cites W2790439813 @default.
• W3217233381 cites W2793264270 @default.
• W3217233381 cites W2793753655 @default.
• W3217233381 cites W2803219518 @default.
• W3217233381 cites W2804230504 @default.
• W3217233381 cites W2806659441 @default.
• W3217233381 cites W2887324181 @default.
• W3217233381 cites W2889826018 @default.
• W3217233381 cites W2895726097 @default.
• W3217233381 cites W2911255357 @default.
• W3217233381 cites W2917758234 @default.
• W3217233381 cites W2921068029 @default.
• W3217233381 cites W2929890274 @default.
• W3217233381 cites W2937446204 @default.
• W3217233381 cites W2939350602 @default.
• W3217233381 cites W2943878465 @default.
• W3217233381 cites W2947248407 @default.
• W3217233381 cites W2947765278 @default.
• W3217233381 cites W2950044547 @default.
• W3217233381 cites W2950896690 @default.
• W3217233381 cites W2952167528 @default.
• W3217233381 cites W2953199440 @default.
• W3217233381 cites W2964106006 @default.
• W3217233381 cites W2970071674 @default.
• W3217233381 cites W2990348250 @default.
• W3217233381 cites W3003557820 @default.
• W3217233381 cites W3025115744 @default.
• W3217233381 cites W3040765226 @default.
• W3217233381 cites W4240924657 @default.
• W3217233381 doi "https://doi.org/10.3389/fmicb.2021.783722" @default.

• next