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- W3217344104 abstract "Many strategies have been reported for the preparation of glycoproteins imprinted polymers, but they take a long time and cannot produce imprinted polymers continuously. Herein, a microfluidic synthesis approach was developed to make glycoproteins imprinted nanospheres rapidly and continuously. By using ovalbumin as a model template and a synthesized phenylboronic acid-tagged silane reagent as the functional monomer, the synthetic conditions including the polymerization contents, the flow rate and the microfluidic reactor size were comprehensively studied. Under the optimized conditions, the glycoprotein imprinted nanospheres could be synthesized rapidly (<2 h), and exhibited high specificity with cross-reactivity factors of 1.3 (ovotransferrin), +∞ (horse-radish peroxidase), 5.1 (β-lactoglobulin) and 101 (bovine serum albumin). The kinetic and equilibrium binding behaviors, reusability and potential applications of the glycoprotein imprinted nanosphere were investigated. Such microfluidic synthesis strategy can be easily extended to produce other target glycoproteins imprinted nanospheres, as well as non-glycoproteins by using suitable functional monomers." @default.
- W3217344104 created "2021-12-06" @default.
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- W3217344104 date "2022-03-01" @default.
- W3217344104 modified "2023-10-17" @default.
- W3217344104 title "A microfluidic approach for rapid and continuous synthesis of glycoprotein-imprinted nanospheres" @default.
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- W3217344104 doi "https://doi.org/10.1016/j.talanta.2021.123084" @default.
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