Matches in SemOpenAlex for { <https://semopenalex.org/work/W3217367789> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W3217367789 abstract "Introduction: Cardiosphere-derived cells (CDCs ) are a population of heart-derived progenitors with demonstrated therapeutic efficacy in preclinical and clinical settings. CDCs exert their effect through the secretion of EVs, lipid-bilayer nanoparticles laden with bioactive molecules. Among the bioactive cargo identified in these immortalized CDC (imCDC)-derived EVs (imCDC-EV) are piwi RNAs (PiRNAs). PiRNAs bind Piwi proteins (members of the argonaute family) and regulate both the epigenome and transcriptome. The role of piRNAs (in particular EV-derived piRNAs) in modulating gene expression remains undescribed. Here we describe the role of a PiRNA highly enriched in imCDC-EVs (imEX-Pi). Methods: EVs were prepared by 24 hours hypoxic conditioning followed by normoxic recovery for three cycles. EVs were isolated from conditioned media using ultrafiltration. Fractions were analyzed for particle size, concentration and piRNA content. In vitro, bone marrow derived-macrophages (BMDM) were exposed to imCDC-EV, imEX-Pi and vehicle for transcriptomic analysis. In vivo, myocardial ischemia-reperfusion (I/R) injury was induced in 8-10-week-old Wistar-Kyoto female rats by LAD coronary ligation for 45 minutes followed by reperfusion. Twenty minutes later, imCDC-EV, imEX-Pi, scramble or vehicle were injected into the left ventricle with an aortic cross-clamp. Cardiac Troponin I levels, infarct size and circulating monocytes were assessed at 24 and 48 hrs post I/R. Results: RNA sequencing showed that piRNAs are enriched in both CDC-EV and imCDC-EV. ImEX-Pi was one of the most highly-expressed non-coding RNAs. In vitro , imEX-Pi altered the transcriptome of BMDMs compared to vehicle. This included upregulation of inflammatory and cell death pathways. In a rat model of I/R injury, imEX-Pi reduced infarct size and cardiac troponin levels compared to vehicle- and scrambled- treated animals. ImEX-Pi also significantly decreased circulating monocytes at 24 hours followed by a significant increase at 48hrs. Conclusions: We show that imEX-Pi is a key mediator of imCDC-EV therapeutic efficacy and that the principle target cells may be macrophages. This is further rationalized by the critical role that macrophages play in the injury and resolution response." @default.
- W3217367789 created "2021-12-06" @default.
- W3217367789 creator A5046381230 @default.
- W3217367789 creator A5059915657 @default.
- W3217367789 creator A5064564309 @default.
- W3217367789 creator A5069334991 @default.
- W3217367789 creator A5075636880 @default.
- W3217367789 date "2021-11-16" @default.
- W3217367789 modified "2023-09-25" @default.
- W3217367789 title "Abstract 11662: Extracellular Vesicle (EV)-Derived PiRNA, ImEX-Pi, is Protective in a Rat Model of Ischemia/Reperfusion Injury" @default.
- W3217367789 doi "https://doi.org/10.1161/circ.144.suppl_1.11662" @default.
- W3217367789 hasPublicationYear "2021" @default.
- W3217367789 type Work @default.
- W3217367789 sameAs 3217367789 @default.
- W3217367789 citedByCount "0" @default.
- W3217367789 crossrefType "journal-article" @default.
- W3217367789 hasAuthorship W3217367789A5046381230 @default.
- W3217367789 hasAuthorship W3217367789A5059915657 @default.
- W3217367789 hasAuthorship W3217367789A5064564309 @default.
- W3217367789 hasAuthorship W3217367789A5069334991 @default.
- W3217367789 hasAuthorship W3217367789A5075636880 @default.
- W3217367789 hasConcept C104317684 @default.
- W3217367789 hasConcept C126322002 @default.
- W3217367789 hasConcept C147724379 @default.
- W3217367789 hasConcept C150194340 @default.
- W3217367789 hasConcept C162317418 @default.
- W3217367789 hasConcept C166703698 @default.
- W3217367789 hasConcept C202751555 @default.
- W3217367789 hasConcept C2780114680 @default.
- W3217367789 hasConcept C2908647359 @default.
- W3217367789 hasConcept C541997718 @default.
- W3217367789 hasConcept C55493867 @default.
- W3217367789 hasConcept C67705224 @default.
- W3217367789 hasConcept C71924100 @default.
- W3217367789 hasConcept C86803240 @default.
- W3217367789 hasConcept C95444343 @default.
- W3217367789 hasConcept C98274493 @default.
- W3217367789 hasConcept C99454951 @default.
- W3217367789 hasConceptScore W3217367789C104317684 @default.
- W3217367789 hasConceptScore W3217367789C126322002 @default.
- W3217367789 hasConceptScore W3217367789C147724379 @default.
- W3217367789 hasConceptScore W3217367789C150194340 @default.
- W3217367789 hasConceptScore W3217367789C162317418 @default.
- W3217367789 hasConceptScore W3217367789C166703698 @default.
- W3217367789 hasConceptScore W3217367789C202751555 @default.
- W3217367789 hasConceptScore W3217367789C2780114680 @default.
- W3217367789 hasConceptScore W3217367789C2908647359 @default.
- W3217367789 hasConceptScore W3217367789C541997718 @default.
- W3217367789 hasConceptScore W3217367789C55493867 @default.
- W3217367789 hasConceptScore W3217367789C67705224 @default.
- W3217367789 hasConceptScore W3217367789C71924100 @default.
- W3217367789 hasConceptScore W3217367789C86803240 @default.
- W3217367789 hasConceptScore W3217367789C95444343 @default.
- W3217367789 hasConceptScore W3217367789C98274493 @default.
- W3217367789 hasConceptScore W3217367789C99454951 @default.
- W3217367789 hasIssue "Suppl_1" @default.
- W3217367789 hasLocation W32173677891 @default.
- W3217367789 hasOpenAccess W3217367789 @default.
- W3217367789 hasPrimaryLocation W32173677891 @default.
- W3217367789 hasRelatedWork W1499958231 @default.
- W3217367789 hasRelatedWork W2411309821 @default.
- W3217367789 hasRelatedWork W2620580060 @default.
- W3217367789 hasRelatedWork W2667910563 @default.
- W3217367789 hasRelatedWork W2767952857 @default.
- W3217367789 hasRelatedWork W2773378880 @default.
- W3217367789 hasRelatedWork W2992622905 @default.
- W3217367789 hasRelatedWork W3015033699 @default.
- W3217367789 hasRelatedWork W3043691326 @default.
- W3217367789 hasRelatedWork W3148766244 @default.
- W3217367789 hasVolume "144" @default.
- W3217367789 isParatext "false" @default.
- W3217367789 isRetracted "false" @default.
- W3217367789 magId "3217367789" @default.
- W3217367789 workType "article" @default.