FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3217378081> ?p ?o ?g. }

• W3217378081 abstract "Background & Aims: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), an enzyme involved in phospholipid acyl-chain remodeling, has been associated with steatosis and hepatic disorders. We examined the relationship between hepatic MBOAT7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2. Results: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammation and MBOAT7 genotype. Mboat7 levels were reduced in murine models of fatty liver, and by hyperinsulinemia. In wild-type mice, Mboat7 was down-regulated by refeeding and insulin, concomitantly with insulin signaling activation. Acute hepatic Mboat7 silencing promoted hepatic steatosis in vivo and enhanced expression of fatty acid transporter Fatp1. MBOAT7-/- HepG2 showed altered phospholipids remodeling consistent with decreased enzymatic activity, increased triglyceride accumulation, enhanced lipogenesis and FATP1 expression, while FATP1 deletion rescued the phenotype. Conclusions: These results suggest that MBOAT7 down-regulation by hyperinsulinemia contributes to hepatic fat accumulation, impairing phospholipids remodeling and up-regulating FATP1.Funding Statement: LV was supported by MyFirst Grant AIRC n.16888, Ricerca Finalizzata Ministero della Salute RF-2016-02364358, Ricerca corrente Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, LV and AG. received funding from the European Union (EU) Programme Horizon 2020 (under grant agreement No. 777377) for the project LITMUS-“ Liver Investigation: Testing Marker Utility in Steatohepatitis”. MM was supported by Fondazione Italiana per lo Studio del Fegato (AISF) ‘Prof. Mario Coppo’ fellowship. AG received funding from the European Union (EU) Programme Horizon 2020 under grant agreement no. 634413 for the project EPoS-“Elucidating Pathways of Steatohepatitis”. Declaration of Interests: The authors declare no conflict of interest relevant to the present manuscript.Ethics Approval Statement: Informed written consent was obtained from each patient and the study protocol was approved by the Ethical Committee of the Fondazione IRCCS Ca’ Granda of Milan and conforms to the ethical guidelines of the 1975 Declaration of Helsinki. We received an approval from an Animal Use Committee for the animal work from the University of Milan and the Italian Ministry of Health Review Boards, in the context of the protocol 8/14, as indicated in the material and methods section in the manuscript. The authorization number of this protocol is 295/2012–A, received on 12/20/2012." @default.
• W3217378081 created "2021-12-06" @default.
• W3217378081 creator A5015477036 @default.
• W3217378081 creator A5020610956 @default.
• W3217378081 creator A5023095216 @default.
• W3217378081 creator A5024750114 @default.
• W3217378081 creator A5035388335 @default.
• W3217378081 creator A5045588634 @default.
• W3217378081 creator A5046105984 @default.
• W3217378081 creator A5048054475 @default.
• W3217378081 creator A5049517101 @default.
• W3217378081 creator A5054314823 @default.
• W3217378081 creator A5054402082 @default.
• W3217378081 creator A5063057369 @default.
• W3217378081 creator A5064556745 @default.
• W3217378081 creator A5075397773 @default.
• W3217378081 creator A5081259222 @default.
• W3217378081 date "2019-01-01" @default.
• W3217378081 modified "2023-09-23" @default.
• W3217378081 title "MBOAT7 Down-Regulation by Hyperinsulinemia Induces Fat Accumulation in Hepatocytes" @default.
• W3217378081 doi "https://doi.org/10.2139/ssrn.3473269" @default.
• W3217378081 hasPublicationYear "2019" @default.
• W3217378081 type Work @default.
• W3217378081 sameAs 3217378081 @default.
• W3217378081 citedByCount "0" @default.
• W3217378081 crossrefType "journal-article" @default.
• W3217378081 hasAuthorship W3217378081A5015477036 @default.
• W3217378081 hasAuthorship W3217378081A5020610956 @default.
• W3217378081 hasAuthorship W3217378081A5023095216 @default.
• W3217378081 hasAuthorship W3217378081A5024750114 @default.
• W3217378081 hasAuthorship W3217378081A5035388335 @default.
• W3217378081 hasAuthorship W3217378081A5045588634 @default.
• W3217378081 hasAuthorship W3217378081A5046105984 @default.
• W3217378081 hasAuthorship W3217378081A5048054475 @default.
• W3217378081 hasAuthorship W3217378081A5049517101 @default.
• W3217378081 hasAuthorship W3217378081A5054314823 @default.
• W3217378081 hasAuthorship W3217378081A5054402082 @default.
• W3217378081 hasAuthorship W3217378081A5063057369 @default.
• W3217378081 hasAuthorship W3217378081A5064556745 @default.
• W3217378081 hasAuthorship W3217378081A5075397773 @default.
• W3217378081 hasAuthorship W3217378081A5081259222 @default.
• W3217378081 hasBestOaLocation W32173780812 @default.
• W3217378081 hasConcept C126322002 @default.
• W3217378081 hasConcept C134018914 @default.
• W3217378081 hasConcept C144133560 @default.
• W3217378081 hasConcept C185592680 @default.
• W3217378081 hasConcept C2777391703 @default.
• W3217378081 hasConcept C2779306644 @default.
• W3217378081 hasConcept C2780988686 @default.
• W3217378081 hasConcept C71924100 @default.
• W3217378081 hasConcept C86803240 @default.
• W3217378081 hasConcept C95444343 @default.
• W3217378081 hasConceptScore W3217378081C126322002 @default.
• W3217378081 hasConceptScore W3217378081C134018914 @default.
• W3217378081 hasConceptScore W3217378081C144133560 @default.
• W3217378081 hasConceptScore W3217378081C185592680 @default.
• W3217378081 hasConceptScore W3217378081C2777391703 @default.
• W3217378081 hasConceptScore W3217378081C2779306644 @default.
• W3217378081 hasConceptScore W3217378081C2780988686 @default.
• W3217378081 hasConceptScore W3217378081C71924100 @default.
• W3217378081 hasConceptScore W3217378081C86803240 @default.
• W3217378081 hasConceptScore W3217378081C95444343 @default.
• W3217378081 hasLocation W32173780811 @default.
• W3217378081 hasLocation W32173780812 @default.
• W3217378081 hasOpenAccess W3217378081 @default.
• W3217378081 hasPrimaryLocation W32173780811 @default.
• W3217378081 hasRelatedWork W1931697203 @default.
• W3217378081 hasRelatedWork W2046764863 @default.
• W3217378081 hasRelatedWork W2047622454 @default.
• W3217378081 hasRelatedWork W2070046754 @default.
• W3217378081 hasRelatedWork W2082995727 @default.
• W3217378081 hasRelatedWork W2145462569 @default.
• W3217378081 hasRelatedWork W2156708180 @default.
• W3217378081 hasRelatedWork W2414819670 @default.
• W3217378081 hasRelatedWork W2584731720 @default.
• W3217378081 hasRelatedWork W4283692114 @default.
• W3217378081 isParatext "false" @default.
• W3217378081 isRetracted "false" @default.
• W3217378081 magId "3217378081" @default.
• W3217378081 workType "article" @default.