FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3217505325> ?p ?o ?g. }

• W3217505325 endingPage "106385" @default.
• W3217505325 startingPage "106385" @default.
• W3217505325 abstract "Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) represent a serious threat to public health and their timely detection is essential for patient management and the prevention of nosocomial infections. Here, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to rapidly identify dominant KPC-Kp in China, by using an automated detection of a KPC-specific peak (at 4521 m/z) by a genetic algorithm using ClinProTools software. Whole-genome sequencing (WGS) was used to understand the genetic environment of the blaKPC-2 gene. In this study, we analyzed 235 K. pneumoniae Chinese clinical isolates, of which 175 (93 KPC-positive isolates and 82 KPC-negative isolates) isolates were used to build a model to select a KPC-specific peak, and another 60 isolates for external validation. In addition, all the spectra were visually inspected by the FlexAnalysis software to evaluate the accuracy of the automated detection. The results showed a 4521 m/z peak found in all blaKPC-2-positive isolates but absent in blaKPC-2-negative isolates. Interestingly, all KPC-Kp belonged to ST11, the dominant clone in China. WGS analysis of a representative isolate showed that the genetic environment of KPC-2 was IS26-ISKpn27-blaKPC-2-ΔISKpn6-Tn1721, similar to the KPC-2 genetic environment of ST11 KPC-Kp previously reported in China. Therefore, the 4521 m/z peak is closely related to ST11 KPC-Kp. In summary, we used MALDI-TOF MS to quickly detect KPC-Kp in the process of routine bacterial identification without increasing costs or requiring further knowledge, which has broad application prospects in drug resistance analysis and infection control." @default.
• W3217505325 created "2021-12-06" @default.
• W3217505325 creator A5007188319 @default.
• W3217505325 creator A5026606987 @default.
• W3217505325 creator A5052960939 @default.
• W3217505325 creator A5064711465 @default.
• W3217505325 creator A5075883325 @default.
• W3217505325 date "2022-01-01" @default.
• W3217505325 modified "2023-09-30" @default.
• W3217505325 title "Rapid detection of KPC-producing Klebsiella pneumoniae in China based on MALDI-TOF MS" @default.
• W3217505325 cites W1460374543 @default.
• W3217505325 cites W1696107782 @default.
• W3217505325 cites W1827979539 @default.
• W3217505325 cites W1903136768 @default.
• W3217505325 cites W1932405830 @default.
• W3217505325 cites W1941330438 @default.
• W3217505325 cites W1951178169 @default.
• W3217505325 cites W1997509815 @default.
• W3217505325 cites W2003265935 @default.
• W3217505325 cites W2005529974 @default.
• W3217505325 cites W2024237409 @default.
• W3217505325 cites W2026379625 @default.
• W3217505325 cites W2041550248 @default.
• W3217505325 cites W2046117506 @default.
• W3217505325 cites W2063910158 @default.
• W3217505325 cites W2075859193 @default.
• W3217505325 cites W2076854301 @default.
• W3217505325 cites W2078043965 @default.
• W3217505325 cites W2096115572 @default.
• W3217505325 cites W2103162870 @default.
• W3217505325 cites W2103171109 @default.
• W3217505325 cites W2105199251 @default.
• W3217505325 cites W2108034897 @default.
• W3217505325 cites W2118177226 @default.
• W3217505325 cites W2120600261 @default.
• W3217505325 cites W2127042507 @default.
• W3217505325 cites W2127148897 @default.
• W3217505325 cites W2134190791 @default.
• W3217505325 cites W2145111808 @default.
• W3217505325 cites W2149560880 @default.
• W3217505325 cites W2150610184 @default.
• W3217505325 cites W2153535438 @default.
• W3217505325 cites W2154955845 @default.
• W3217505325 cites W2169680194 @default.
• W3217505325 cites W2294303797 @default.
• W3217505325 cites W2341490520 @default.
• W3217505325 cites W2353546921 @default.
• W3217505325 cites W2400586161 @default.
• W3217505325 cites W2508745223 @default.
• W3217505325 cites W2522411258 @default.
• W3217505325 cites W2565726837 @default.
• W3217505325 cites W2578718824 @default.
• W3217505325 cites W2586692366 @default.
• W3217505325 cites W2593840352 @default.
• W3217505325 cites W2726778476 @default.
• W3217505325 cites W2728170331 @default.
• W3217505325 cites W2735197607 @default.
• W3217505325 cites W2779019546 @default.
• W3217505325 cites W2787803146 @default.
• W3217505325 cites W2801385467 @default.
• W3217505325 cites W2888869275 @default.
• W3217505325 cites W2889354016 @default.
• W3217505325 cites W2903554743 @default.
• W3217505325 cites W2905366281 @default.
• W3217505325 cites W2907564207 @default.
• W3217505325 cites W2918778451 @default.
• W3217505325 cites W2922018914 @default.
• W3217505325 cites W2922037673 @default.
• W3217505325 cites W2952167528 @default.
• W3217505325 cites W2969847687 @default.
• W3217505325 cites W2973643612 @default.
• W3217505325 cites W2983920188 @default.
• W3217505325 cites W2986910725 @default.
• W3217505325 cites W2987898767 @default.
• W3217505325 cites W3015025018 @default.
• W3217505325 cites W3028438231 @default.
• W3217505325 cites W3089112430 @default.
• W3217505325 cites W3162370580 @default.
• W3217505325 cites W3184860359 @default.
• W3217505325 cites W3194004472 @default.
• W3217505325 doi "https://doi.org/10.1016/j.mimet.2021.106385" @default.
• W3217505325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34843862" @default.
• W3217505325 hasPublicationYear "2022" @default.
• W3217505325 type Work @default.
• W3217505325 sameAs 3217505325 @default.
• W3217505325 citedByCount "9" @default.
• W3217505325 countsByYear W32175053252022 @default.
• W3217505325 countsByYear W32175053252023 @default.
• W3217505325 crossrefType "journal-article" @default.
• W3217505325 hasAuthorship W3217505325A5007188319 @default.
• W3217505325 hasAuthorship W3217505325A5026606987 @default.
• W3217505325 hasAuthorship W3217505325A5052960939 @default.
• W3217505325 hasAuthorship W3217505325A5064711465 @default.
• W3217505325 hasAuthorship W3217505325A5075883325 @default.
• W3217505325 hasBestOaLocation W32175053251 @default.
• W3217505325 hasConcept C104317684 @default.
• W3217505325 hasConcept C2777058267 @default.
• W3217505325 hasConcept C54355233 @default.

• next