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• W3217728051 abstract "Iatrogenic preterm premature rupture of the membrane remains the Achille's heel of fetoscopy. The aim of this study was to show in vivo feasibility of fetal membrane (FM) defect sealing by the application of tissue glues with umbrella-shaped receptors.First, we adapted our previously described ex vivo strategy and evaluated the adhesion strength of different tissue glues, Histoacryl® and Glubran2®, by bonding polytetrafluoroethylene or silicone encapsulated nitinol glue receptor to human FM. Then, we exposed pregnant sheep uterus through a laparotomy and placed a 10-French trocar into the amniotic cavity through which the umbrella-shaped glue receptor (n = 9) was inserted and fixated onto the FM with the tissue glues (n = 8). The tightness of the sealed defects was assessed 4 h post-surgery.Both tissue glues tested resulted in adhesion of the glue receptors to the FM ex vivo. In vivo, all glue receptors opened in the amniotic cavity (n = 9) and all successfully placed glue receptors sealed the FM defect (n = 8). Four hours post-surgery, 2 treatment sites showed minimal leakage whereas the negative control without glue (n = 1) showed substantial leakage.This in vivo study confirms that fetoscopically induced FM defects can be sealed by the application of tissue adhesives." @default.
• W3217728051 created "2021-12-06" @default.
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• W3217728051 date "2021-01-01" @default.
• W3217728051 modified "2023-10-12" @default.
• W3217728051 title "Minimally Invasive Precise Application of Bioadhesives to Prevent IPPROM on a Pregnant Sheep Model" @default.
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• W3217728051 doi "https://doi.org/10.1159/000519910" @default.
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